Molecular characterization of two dsRNAs that could correspond to the genome of a new mycovirus that infects the entomopathogenic fungus Beauveria bassiana

Prevalence and species diversity of dsRNA mycoviruses from Beauveria bassiana strains in the China's Guniujiang nature

“…The advent of high-throughput NGS technologies and metatranscriptomics based on RNA sequencing (RNA-seq) has overcome challenges associated with virus identification and significantly expanded our knowledge of virome and mixed viral infections in single fungal strains [ [9] , [10] , [11] , [12] , [13] ]. Recent studies have increasingly revealed mixed infections of different mycoviruses or dsRNA elements within a single strain, such as in fungal strains B. bassiana , Rosellinia necatrix , Botrytis cinerea , and Rhizoctonia solani [ 9 , 10 , 14 , 15 ]. For instance, Rhizoctonia solani strain DC17 was found to be co-infected with 17 different mycoviruses [ 10 ], while a B. bassiana strain RCEF1446 was found to harbor three mycoviruses [ 14 ].…”

“…Recent studies have increasingly revealed mixed infections of different mycoviruses or dsRNA elements within a single strain, such as in fungal strains B. bassiana , Rosellinia necatrix , Botrytis cinerea , and Rhizoctonia solani [ 9 , 10 , 14 , 15 ]. For instance, Rhizoctonia solani strain DC17 was found to be co-infected with 17 different mycoviruses [ 10 ], while a B. bassiana strain RCEF1446 was found to harbor three mycoviruses [ 14 ].…”

A novel narnavirus from the entomogenous fungus Beauveria bassiana Vuillemin

“…The only Narna-like virus BbSNLV has been found in B. bassiana, which harbors a single stranded RNA encoding a RdRp [14] , while there has no report of Narnavirus with multiple segments in B. bassiana, although some Narna-like viruses with multiple segments have been identi ed from other fungus, for instance, four Narna-like viruses isolated from Sclerotinia sclerotiorum possessed two to four segments [20] . The Aspergillus tennesseensis narnavirus 1, belongs to the well-established genus Narnavirus, possesses of ve segments, both the ssRNA1 and ssRNA2 of which encoded two different RdRp motifs, respectively [21] .…”

“…Previous studies have shown that B. bassiana could be infected by mycoviruses, with some viruses had no in uence on it, while others could affect its biological characteristics and pathogenicity to insect pests [12,13] . So far, it has been reported that double stranded RNA viruses (dsRNA) were universal in B. bassiana [14,15] , but there are few records of ssRNA mycovirus, especially Narnavirus, although a single stranded small Narna-like virus, Beauveria bassiana small Narna-like virus (BbSNLV) was reported by Kotta-Loizou and Coutts in 2017 [15] . In the present study, we identi ed a novel +ssRNA virus with typical characteristic of splipalmiviruses belonging to Splipalmiviridae (likely a new family) and suggested temporarily nominating it as Beauveria bassiana splipalmivirus (BbSpV1).…”