Molecular characterization of two isoforms of ZFAND3 cDNA from the Japanese quail and the leopard gecko, and different expression patterns between testis and ovary

Otake, Shigeo; Endo, Daisuke; Park, Min Kyun

doi:10.1016/j.gene.2011.08.021

Cited by 8 publications

(14 citation statements)

References 28 publications

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“…To examine the expression sites of AMH and germ cell markers (DMC1 and ZFAND3) in the testis of the Japanese quail, in situ hybridization was performed as described previously (Otake et al 2011) with some modifications as follows. The paraffin sections were treated with Proteinase K (1 μg/mL, 30 min, 37°C) and hybridized with 1 μg/mL sense and antisense RNA probes.…”

Section: In Situ Hybridization and Immunohistochemistrymentioning

confidence: 99%

“…Immunohistochemistry of DDX4 (also known as VASA) was performed in the same section to identify the germ cells in the seminiferous tubules. The expressions of the spermatocyte marker DMC1 (Yoshida et al 1998) and spermatid marker ZFAND3 (also known as TEX27, Otake et al 2011) were also analyzed to examine the germ cell types in the regressed testis. The ORF sequence of DMC1 was identified from the quail for probe synthesis (GenBank accession number: KU975604).…”

Section: Identification Of Amh-expressing Cells In the Testismentioning

confidence: 99%

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Expressional changes of AMH signaling system in the quail testis induced by photoperiod

Otake

Park

2016

Reproduction

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Gonadal sex differentiation proceeds by the interplay of various genes including the transcription factors and secretory factors in a complex network. The sex-differentiating genes are expressed not only during early sex differentiation but also throughout the gonadal development and even in the adult gonads. In addition, the evidence that they actually function in the adult gonads have been accumulated from the studies using the conditional knockout mice. However, many previous studies were focused on one single gene though those genes function in a network. In this study, the expressions of various sex-differentiating genes were analyzed simultaneously in the adult testis of the Japanese quail (Coturnix japonica), whose testicular functions are dramatically changed by altering the photoperiod, to elucidate the roles of them in the adult gonad. Anti-Müllerian hormone (AMH) was significantly upregulated in the regressed testis induced by the short-day condition. The expressions of the transcription factors that promote AMH expression in mammals (SF1, SOX9, WT1 and GATA4) were also increased in the regressed testis. Moreover, AMH receptor (AMHR2) showed similar expression pattern to its ligand. We also analyzed the expressions of other transforming growth factor beta (TGFB) superfamily members and their receptors. The expressions of the ligands and receptors of TGFB family, and follistatin and betaglycan in addition to inhibin subunits were increased in the regressed testis. These results suggest that AMH is involved in the adult testicular functions of the Japanese quail together with other TGFB superfamily members.

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Section: In Situ Hybridization and Immunohistochemistrymentioning

confidence: 99%

Section: Identification Of Amh-expressing Cells In the Testismentioning

confidence: 99%

Expressional changes of AMH signaling system in the quail testis induced by photoperiod

Otake

Park

2016

Reproduction

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“…In humans, recent study showed that ZFAND3 is also involved in type II diabetes 11 . Although molecular cloning of ZFAND3 cDNA has been performed in vertebrates, such as reptiles 12 and amphibians 9 , the function of ZFAND3 remains largely unclear in fish.…”

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confidence: 99%

Charactering the ZFAND3 gene mapped in the sex-determining locus in hybrid tilapia (Oreochromis spp.)

Knepper

Liao

Liu

et al. 2016

Sci Rep

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Zinc finger AN1-type domain 3 (ZFAND3) is essential for spermatogenesis in mice. However, its function in teleosts remains unclear. In this study, we characterized the ZFAND3 gene (termed as OsZFAND3) in an important food fish, tilapia. The OsZFAND3 cDNA sequence is 1,050 bp in length, containing an ORF of 615 bp, which encodes a putative peptide of 204 amino acid residues. Quantitative real-time PCR revealed that the OsZFAND3 transcripts were exclusively expressed in the testis and ovary. In situ hybridization showed that the high expression of OsZFAND3 transcripts was predominantly localized in the spermatocyte and spermatid. These results suggest that OsZFAND3 is involved in male germ cell maturation. Three single nucleotide polymorphisms (SNPs) were detected in the introns of OsZFAND3. The OsZFAND3 gene was mapped in the sex-determining locus on linkage group 1 (LG1). The three SNPs in the OsZFAND3 gene were strictly associated with sex phenotype, suggesting that the OsZFAND3 gene is tightly linked to the sex-determining locus. Our study provides new insights into the functions of the OsZFAND3 gene in tilapia and a foundation for further detailed analysis of the OsZFAND3 gene in sex determination and differentiation.

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“…ZFAND3 is present in post-meiotic cells during spermatogenesis [9]. Moreover, ZFAND3 mRNA is expressed primarily in spermatids in the testis and oocytes in the ovary [29]. It is accepted that ZFAND3 is a useful marker of spermatogenesis, because ZFAND3 has a critical physiological function related to germ cell maturation [29].…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, ZFAND3 mRNA is expressed primarily in spermatids in the testis and oocytes in the ovary [29]. It is accepted that ZFAND3 is a useful marker of spermatogenesis, because ZFAND3 has a critical physiological function related to germ cell maturation [29]. Thus, the regulation of ZFAND3 expression in the testis is critical for spermatogenesis, and a decrease in ZFAND3 expression may lead to the inactivation of spermatogenesis [9].…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Testicular Ischemia-Reperfusion Injury in Rats

Ouh

Seo

Shah

et al. 2014

The Journal of Veterinary Medical Science

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Testicular torsion is a urological emergency that leads to serious testicular damage and male infertility. We performed this study to identify specific proteins that are differentially expressed in response to testicular torsion and detorsion-induced ischemia-reperfusion (I-R) injury. Adult male rats were divided into two groups: a sham-operated group and a testicular I-R group. Testicular torsion was induced by rotating the left testis 720° in a clockwise direction for 1 hr, and then, detorsion was performed for 24 hr. After this testicular tissues were collected, protein analysis was performed using two-dimensional gel electrophoresis and Western blot analyses. Testicular I-R injury resulted in serious histopathologic damage to the germinal cells in the seminiferous tubules and increased the number of TUNEL-positive cells in testicular tissue. Specific protein spots with a greater than 2.5-fold change in intensity between the sham-operated and testicular I-R groups were identified by mass spectrometry. Among these proteins, levels of peroxiredoxin 6, thioredoxin, heterogeneous nuclear ribonucleoproteins, ubiquitin carboxyl terminal hydrolase isozyme L5 and zinc finger AN1-type domain 3 were decreased in the testicular I-R group compared to the sham-operated group. Moreover, Western blot analysis clearly showed the decrease of these proteins in the testicular I-R group. These proteins have spermatogenesis and anti-oxidative functions. These findings suggest that testicular I-R results in cell death due to altered expression of several proteins with spermatogenesis and anti-oxidation functions.

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Molecular characterization of two isoforms of ZFAND3 cDNA from the Japanese quail and the leopard gecko, and different expression patterns between testis and ovary

Cited by 8 publications

References 28 publications

Expressional changes of AMH signaling system in the quail testis induced by photoperiod

Expressional changes of AMH signaling system in the quail testis induced by photoperiod

Charactering the ZFAND3 gene mapped in the sex-determining locus in hybrid tilapia (Oreochromis spp.)

Proteomic Analysis of Testicular Ischemia-Reperfusion Injury in Rats

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