Molecular characterization of two natural hybrids from the Iberian Peninsula: Ranunculus × luizetii and R. × peredae (Ranunculaceae)

Abstract

Two nothospecies, Ranunculus ×luizetii and R. ×peredae (Ranunculaceae), were analyzed and discussed. For this purpose, Amplified Fragment Length Polymorphism (AFLP) markers, nuclear rDNA sequences (ITS1, 5.8S and ITS2) and pollen viability were conducted. The profiles of these hybrid samples were compared to their putative progenitors. Several additive polymorphic sites detected in the ITS sequences of the hybrid samples (R. ×luizetii and R. ×peredae) also confirmed their derived origins from ribotypes of their parental taxa (R. parnassiifolius subsp. parnassiifolius × R. pyrenaeus; R. amplexicaulis × R. cabrerensis subsp. cabrerensis, respectively). Despite the lack of exclusive AFLP markers reported in both hybrids, presumably due to effects of introgression, the concerted evolution of many rDNA polymorphisms towards either of the parental ribotypes indicated their ancient origin. Pollen fertility estimation in R. ×luizetii presented a mean value of 60.58%, which showed that hybrid samples are well established and fertile. However, a larger difference was observed in R. ×peredae, where the mean value of pollen fertility was very low (18.91%).

Inhibitory Effect of Ranunculus kotschyi Boiss. Extract on Multidrug Resistant Acinetobacter baumanii and Other Pathogenic Bacteria

“…Genomic DNA was extracted from 30 mg dried plant tissue by DNeasy Plant Mini Kit (Qiagen, Germany). PCR reactions were made according to previously established procedures (Cires et al, 2012). Briefly; 50 μL of reaction mixture, containing 50 ng of template DNA, 5 μL of 10X reaction buffer, 2.5 mM MgCl2, 0.2 mM dNTPs, 1 U Taq DNA polymerase (Sigma) and 0.08 μM each of primers, was run in thermocycler.…”