Molecular characterization of viruses found in honeybee (Apis mellifera) colonies infested with Varroa destructor and Nosema cerana in Egypt

Abd-El-Samie, Emtithal M.; Basuny, Nasr K.; Seyam, Heba

doi:10.1016/j.mcp.2021.101731

Cited by 8 publications

(3 citation statements)

References 32 publications

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“…Despite the fact that DWV-B is better adapted than DWV-A for transmission by V. destructor [ 28 ], our interpretation for the lack of DWV-B in our study is that Egypt prohibits honey bee importation and that DWV-B may not yet have arrived in the country or, if it has, then only in a limited number of apiaries. It suggests that, if present in the country [ 21 ], DWV-B remains at very low prevalence and at a very low intensity of infection.…”

Section: Discussionmentioning

confidence: 99%

“…Many countries have reported high annual colony losses caused by multiple stressors [ 19 ], including DWV, now widespread across the world [ 20 ]. Although numerous studies have been conducted to assess the prevalence of viruses in honey bee colonies around the world [ 9 ], there has only been one study to date that has screened some honey bee viruses in Egyptian bee colonies [ 21 ], and which reported that BQCV, DWV-A, and IAPV were widespread, whilst DWV-B was present in Egyptian honey bees, though without specifying the geographic distribution of these viruses. As a result, more comprehensive studies involving different geographical regions and seasons are required to determine the prevalence of bee viruses in Egyptian bee colonies in order to understand and interpret observed colony losses.…”

Section: Introductionmentioning

confidence: 99%

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Nationwide Screening for Bee Viruses in Apis mellifera Colonies in Egypt

Kandel

Paxton

Naggar

2023

Insects

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Honey bees are essential for crop and wild plant pollination. However, many countries have reported high annual colony losses caused by multiple possible stressors. Diseases, particularly those caused by viruses, are a major cause of colony losses. However, little is known about the prevalence of honey bee pathogens, particularly virus prevalence, in Egyptian honey bees. To address this shortfall, we determined the prevalence of widespread bee viruses in honey bee colonies in Egypt—whether it is affected by geography, the season, or infestation with Varroa destructor (varroa) mites. Honey bee worker samples were collected from 18 geographical regions across Egypt during two seasons: winter and summer of 2021. Three apiaries were chosen in each region, and a pooled sample of 150 worker bees was collected from five colonies in each apiary then screened by qPCR for 10 viral targets: acute bee paralysis virus (ABPV), black queen cell virus (BQCV), chronic bee paralysis virus (CBPV), deformed wing virus (DWV) genotypes A (DWV-A), B (DWV-B) and D (Egyptian bee virus), Israeli acute paralysis virus (IAPV), Kashmir bee virus (KBV), sacbrood virus (SBV), and slow bee paralysis virus (SBPV). Our results revealed that DWV-A was the most prevalent virus, followed by BQCV and ABPV; the DWV genotype now spreading across the world, DWV-B, was not detected. There was no difference in varroa infestation rates as well as virus prevalence between winter and summer. However, colonies infected with BQCV had a significantly higher varroa count (adjusted p < 0.05) in the winter season, indicating that there is a seasonal association between the intensity of infestation by varroa and the presence of this virus. We provide data on the current virus prevalence in Egypt, which could assist in the protection of Egypt’s beekeeping industry. Moreover, our study aids in the systematic assessment of the global honey bee virome by filling a knowledge gap about the prevalence of honey bee viruses in Egypt.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nationwide Screening for Bee Viruses in Apis mellifera Colonies in Egypt

Kandel

Paxton

Naggar

2023

Insects

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“…Until 2008, Nosema apis was the only diagnosed species of the microsporidian parasite in Slovak honey bees causing diarrhea [ 8 ]. This disease is often associated with CCD—colony collapse disorder [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Detection of Nosema spp. in Three Eco Regions of Slovakia

Hurná,

Sučik,

Staroň

et al. 2023

CIMB

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Microsporidia are unicellular obligate intracellular parasitic fungi that infect a wide range of vertebrates and invertebrates. There are two known species of microsporidia infecting honey bees in Slovakia- first Nosema apis and also Nosema ceranae. Our aim was to examine samples of honey bees collected from bee queen breeders in three ecoregions of the Slovak Republic in 2021 and 2022. First, microscopic diagnostics were used, and then randomly selected samples were examined using molecular methods. There were 4018 samples examined using microscopic diagnostics and the positivity was demonstrated in 922 samples. From the microscopically diagnosed positive samples, 507 samples were randomly selected, and using molecular methods, the positivity was proved in 488 samples. After sequencing the positive PCR products and comparing the sequences (BLAST) with the sequences stored in the gene bank, the Nosema ceranae species was detected in all positive samples.

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Design and implementation of a smart beehive and its monitoring system using microservices in the context of IoT and open data

Aydın

2022

Computers and Electronics in Agriculture

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Molecular characterization of viruses found in honeybee (Apis mellifera) colonies infested with Varroa destructor and Nosema cerana in Egypt

Cited by 8 publications

References 32 publications

Nationwide Screening for Bee Viruses in Apis mellifera Colonies in Egypt

Nationwide Screening for Bee Viruses in Apis mellifera Colonies in Egypt

Molecular Detection of Nosema spp. in Three Eco Regions of Slovakia

Design and implementation of a smart beehive and its monitoring system using microservices in the context of IoT and open data

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