Molecular characterization of VP4 and NSP4 genes from rotavirus strains infecting neonates and young children in Belém, Brazil

Mascarenhas, Joana D’Arc Pereira; Linhares, Alexandre C.; Gabbay, Yvone Benchimol; Lima, Clarissa Silva; Guerra, Sylvia de Fátima Santos; Soares, Luana da Silva; Oliveira, Darleise S.; Lima, Jackson Cordeiro; Macêdo, Olinda; Leite, José Paulo Gagliardi

doi:10.1016/j.virusres.2007.02.010

Cited by 29 publications

(27 citation statements)

References 57 publications

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“…The phylogenetic clustering pattern we found is in agreement with analysis done on the VP8* gene region of neonatal P[6] strains isolated in Brazil (Mascarenhas et al, 2007). Using amino acid alignments, we found that the VP4 proteins of neonatal P[6] RVs tend to differ at several positions compared with P[6] strains isolated from older infants for children.…”

Section: Discussionsupporting

confidence: 88%

Complete genome sequence analysis of candidate human rotavirus vaccine strains RV3 and 116E

2010

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Rotaviruses (RVs) cause severe gastroenteritis in infants and young children; yet, several strains have been isolated from newborns showing no signs of clinical illness. Two of these neonatal strains, RV3 (G3P[6]) and 116E (G9P[11]), are currently being developed as live-attenuated vaccines. In this study, we sequenced the eleven-segmented double-stranded RNA genomes of cell culture-adapted RV3 and 116E and compared their genes and protein products to those of other RVs. Using amino acid alignments and structural predictions, we identified residues of RV3 or 116E that may contribute to attenuation or influence vaccine efficacy. We also discovered residues of the VP4 attachment protein that correlate with the capacity of some P[6] strains, including RV3, to infect newborns versus older infants. The results of this study enhance our understanding of the molecular determinants of RV3 and 116E attenuation and are expected to aid in the ongoing development of these vaccine candidates.

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Section: Discussionsupporting

confidence: 88%

Complete genome sequence analysis of candidate human rotavirus vaccine strains RV3 and 116E

2010

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“…Detection of human-like VP6 gene in PoRVCs is in agreement with the report of Gabbay et al (2008). It is possible that animals might serve as reservoir host for re-assortment of RVs from different host origin (Mascarenhas et al 2007;Gabbay et al 2008;Navarro et al 2017). RVC infection in pigs becomes more important as the main livelihood framework of rural and tribal population that dominates the northeastern states of India is piggery.…”

Section: Discussionsupporting

confidence: 82%

Unexpected detection of porcine rotavirus C strains carrying human origin VP6 gene

Kattoor

Saurabh

Malik

et al. 2017

Veterinary Quarterly

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Background: Rotavirus C (RVC), a known etiological agent of diarrheal outbreaks, mainly inflicts swine population globally with sporadic incidence in human, cattle, ferret, mink and dog. Objective: To demonstrate the presence of RVC in Indian swine population and characterization of its selected structural (VP6) and non-structural (NSP4 and NSP5) genes. Methods: A total of 108 diarrheic samples from different regions of India were used. Isolated RNA was loaded onto polyacrylamide gel to screen for the presence of RVs through the identification of specific electrophoretic genomic migration pattern. To characterize the RVC strains, VP6 gene and NSP4 and NSP5 genes were amplified, sequenced and analyzed. Results: Based on VP6 gene specific diagnostic RT-PCR, the presence of RVC was confirmed in 12.0% (13/108) piglet fecal specimens. The nucleotide sequence analysis of VP6 gene, encoding inner capsid protein, from selected porcine RVC (PoRVC) strains revealed more than 93% homologies to human RVC strains (HuRVC) of Eurasian origin. These strains were distant from hitherto reported PoRVCs and clustered with HuRVCs, owning I2 genotype. However, the two non-structural genes, i.e. NSP4 and NSP5, of these strains were found to be of swine type, signifying a re-assortment event that has occurred in the Indian swine population. Conclusion:The findings indicate the presence of human-like RVC in Indian pigs and division of RVC clade with I2 genotype into further sub-clades. To the best of our knowledge, this appears to be the first report of RVC in Indian swine population. Incidence of human-like RVC VP6 gene in swine supports its subsequent zoonotic prospective.

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“…11,12 However, in Brazil, this is true for the southeastern area, 38 but not for the northern region, where A type is prevalent. 39 The data reported in our work demonstrated similar profile of the wild circulating strains of RV, as compared to data obtained elsewhere in the world and in our country. Molecular and serological nuances are mainly attributed to factors, such as RV genotypic and phenotypic variability; interspecies barriers crossing; host immunity pressure -naturally or artificially acquired, as well as, geographical and seasonal features.…”

Section: Discussionsupporting

confidence: 88%

Detection and characterization of human rotavirus in hospitalized patients in the cities of Ponta Grossa, Londrina and Assai - PR, Brazil

Nozawa¹,

Kerntopf²,

Czernisz³

et al. 2010

Braz J Infect Dis

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Acute diarrheal disease is still one of the major public health problems worldwide. Rotaviruses (RV) are the most important viral etiologic agents and children under fi ve years of age are the target population. Objective: To investigate the rate of RV infection in hospitalized patients due to acute diarrhea in the cities of Ponta Grossa, Londrina and Assai -Paraná. Methods: Latex agglutination (LA); immunochromatography (ICG); polyacrylamide gel electrophoresis (PAGE) and negative staining electron microscopy (ME) tests were used to detect the virus. For the genotyping, RT-PCR and RT-PCR-ELISA were used, respectively, for NSP4 and VP4/VP7. Result: Out of 124 samples there were 69 positive stool samples for RV, for at least one of the used tests, 67 of them being RV group A (RV-A). Overall, most of the RV positive stool samples came from children under thirteen years of age. However, 12 positive cases occurred in patients aged 13 years or above, including an 81-year old patient. Conclusion: The data showed similar electropherotypes and genotypes G, P and NSP4 of the inland wild circulating strains of RV.

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Molecular characterization of VP4 and NSP4 genes from rotavirus strains infecting neonates and young children in Belém, Brazil

Cited by 29 publications

References 57 publications

Complete genome sequence analysis of candidate human rotavirus vaccine strains RV3 and 116E

Complete genome sequence analysis of candidate human rotavirus vaccine strains RV3 and 116E

Unexpected detection of porcine rotavirus C strains carrying human origin VP6 gene

Detection and characterization of human rotavirus in hospitalized patients in the cities of Ponta Grossa, Londrina and Assai - PR, Brazil

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