Molecular characterization of watermelon cultivars using microsatellite markers

Gama, Renata Natália Cs; Santos, C. A. F.; Dias, R. de C. S.; Souza, Flávio F. de

doi:10.1590/s0102-05362013000400003

Cited by 6 publications

(5 citation statements)

References 16 publications

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“…These primers can be used in further research with the cultivars analyzed in this study, as well as in their differentiation, which can be an important tool in the useful and fast distinction of genotypes. Gama et al (2013) used 10 SSR primers to characterize 17 watermelon cultivars and amplified a total of 34 alleles with similarity indexes ranging from 0.34 to 1 concluding that the analyzed loci were not sufficient to distinguish these cultivars.…”

Section: Resultsmentioning

confidence: 99%

Research Article Analysis of genetic variability of commercial melon cultivars using SSR molecular markers.

Carvalho¹,

Canela²,

Leite³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Taking into account the scenario of melon production (Cucumis melo L.) in Brazil, it is notable the importance of the culture in the fruit market. The preference of the national market is concentrated in the fruits of the yellow type. Therefore, it is possible that the genetic base of the commercial cultivars is relatively narrow due to the loss of important genes in the breeding process, and it is possible to introgress external genes for increasing recombination possibilities that generate sources of resistance to the main diseases and superior genotypes. This study evaluated the genetic variability of 87 melon accessions composed of 72 commercial cultivars belonging to the Inodorus and Cantaloupensis groups and 15 plant introductions (PIs). Forty-four polymorphic SSR primers were used to amplify a total of 202 alleles. The expected heterozygosity presented an average of 0.519 and the PIC index of 0.458, being moderately informative. The dendrogram generated for the 72 cultivars grouped the genotypes into 2 main groups, and there was no association with the classification of groups of the genotypes in the grouping. The number of SSR markers was sufficient to predict wide genetic variability among the cultivars studied, with the similarity between 0.35 and 0.98. The dendrogram presented for the 72 cultivars and the 15 PI genotypes was not associated with the classification of the genotypes in the grouping, and the 15 PIs were well dispersed with similarity indexes that resemble the two groups studied. A set of 25 primers was identified that were useful in distinguishing the 72 cultivars. These primers can be used in later research with the cultivars, as well as in crop protection situations, being an important tool in the useful and rapid distinction of genotypes, and in commercial disputes regarding the certification of the main melon cultivars used in the country.

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Section: Resultsmentioning

confidence: 99%

Research Article Analysis of genetic variability of commercial melon cultivars using SSR molecular markers.

Carvalho¹,

Canela²,

Leite³

et al. 2017

Genet. Mol. Res.

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“…Genetic studies have been successfully carried out in plant species using different molecular marker techniques (Coskun et al, 2017;Karaman et al, 2018;Uzun et al, 2020;Aslan et al, 2021;Morilipınar et al, 2021;Kırac et al, 2022). Genetic characterization studies were performed using SSR markers in different watermelon genotypes (Guerra-Sanz, 2002;Solmaz, 2010;Zhang et al, 2012;Gama et al, 2013;Kwon, 2013;Nantoume et al, 2013;Kong et al, 2014;Lu et al, 2018).…”

Section: Research Articlementioning

confidence: 99%

Microsatellite analysis in some watermelon (Citrullus lanatus) genotypes

KAMAŞAK¹,

Coşkun

Şekerci³

et al. 2022

International Journal of Agriculture Environment and Food Sciences

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Conservation of genetic resources is essential for the continuation of future crop production. Watermelon (Citrul-lus lanatus), a member of Cucurbitaceae, is widely distributed in tropical and subtropical regions. The aim of this study was to reveal the genetic relationships with the help of microsatellite markers in a watermelon collection free of unnecessary repetitions, and to determine the success of SSR (Simple Sequence Repeats) primers developed in cucurbits. In this study, 96 watermelon genotypes with good agronomic characteristics were used among the geno types collected from different regions of Turkey and purified up to the S4-S6 (self-pollination) stage. In the study, 33 SSR primer pairs were used to determine the genetic relationship between watermelon genotypes. In the study, a total of 67 bands were obtained with SSR primers. As a result of UPGMA (Unweighted Pair Group Method Using Arithmetic Averages) analysis, genotypes showed similarity at the level of 0.84-1.00. The number of alleles detected per primer varied between 1 and 6. In terms of the total number of alleles obtained, CMCT44 (5 units) and Cgb4767 (6 units) loci produced the most alleles. Primers with high polymorphism rate and allele excess were determined, and the possibilities for use in genetic stability analyses, variety differentiation and other genetic analyses were determined.

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“…These SSRs were selected based on the study by Gama et al (2013), because they exhibited PCR products of different sizes for these cultivars. The PCR was conducted by following the protocol of Joobeur et al (2004), with some modifications: the total volume of 12 µL contained 50 ng DNA, 5 µM of each primer, 0.1 mM dNTP, 1.5 mM MgCl 2 , 1X PCR buffer, and 0.75 U Taq polymerase DNA enzyme.…”

Section: Dna Extraction and Microsatellite Analyses Of The F1smentioning

confidence: 99%

“…There are various cultivars of this species, which differ from one another in terms of the external pattern of the fruit, the color of the pulp, the level of soluble solids, the yield, resistance to pests and diseases, and precocity, among other desirable agronomic traits. A summary of these morphological traits is provided by Dias et al (2010) and Gama et al (2013).…”

Section: Introductionmentioning

confidence: 99%

“…They have been developed for various cultivated plant species and are quickly replacing other marker types in various types of genetic studies, because of their reproducibility and technical simplicity, the small amount of DNA that is required, their low cost, their great resolving power, and their high levels of polymorphism (Caixeta et al, 2009). Gama et al (2013) used 36 molecular markers to characterize 17 watermelon cultivars, and observed the formation of two groups, one that contained cultivars that exhibited a diffuse stripe pattern, and one that contained cultivars that exhibited a well-defined stripe pattern.…”

Section: Introductionmentioning

confidence: 99%

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Microsatellite markers linked to the locus of the watermelon fruit stripe pattern

et al. 2015

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ABSTRACT. Agronomic performance and external and internal appearance of watermelon (Citrullus lanatus) fruit are important traits that should be taken into consideration during the development of a new cultivar, as well as being the principal identification elements used by the consumer, which are based on the external appearance and quality of the fruit. Externally, the fruit can be characterized in terms of the shape, the color of the lower rind, and the presence of grooves and stripes, the stripes can be classified as clearly defined or diffuse. The objective of this study was to identify microsatellite markers linked to the stripe pattern of watermelon fruit to support watermelon improvement programs, with the selection of this characteristic in the plantlet stage. F1 and F2 populations, result of a cross between the cultivars BRS Opara (clearly defined stripes) and Pérola (diffuse stripes), were phenotyped for their fruit stripe pattern. The CTAB 2X protocol was used for DNA extraction and 116 microsatellite markers were examined in a group of F2 plants that had fruit with well-defined stripes and fruit with diffuse stripes. The microsatellite loci MCPI_05 and MCPI_16 exhibited a linkage to the stripe pattern at a distance of 1.5 and 1.8 cM, respectively, with LOD scores of 39.28 and 38.11, respectively, which were located on chromosome six of the watermelon genome. These markers can be used in marker-assisted selection in watermelon improvement programs, by various research institutions.

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Molecular characterization of watermelon cultivars using microsatellite markers

Cited by 6 publications

References 16 publications

Research Article Analysis of genetic variability of commercial melon cultivars using SSR molecular markers.

Research Article Analysis of genetic variability of commercial melon cultivars using SSR molecular markers.

Microsatellite analysis in some watermelon (Citrullus lanatus) genotypes

Microsatellite markers linked to the locus of the watermelon fruit stripe pattern

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