2022
DOI: 10.1111/jph.13157
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Molecular characterization of wild tomato mosaic virus and chilli veinal mottle virus mix‐infecting chilli pepper in China

Abstract: Chilli pepper (Capsicum annuum L.) is an economically important crop worldwide. However, numerous plant pathogens, including potyviruses, affect its production (Kenyon et al., 2014;Parisi et al., 2020).Potyviruses are aphid-transmitted plant viruses belonging to the genus Potyvirus. Potyvirus virion is a flexuous filamentous capsid with a length of 700-900 nm and a diameter of 11-13 nm. The potyvirus genome consists of a 9.7 Kb single-stranded RNA (ssRNA), two untranslated regions in 5'-UTR and 3'-UTR, and a s… Show more

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“…To further identify the species of virus, the P. kingianum sample was sent to Ouyi Co., Ltd. (Shanghai, China) for next-generation sequencing (NGS), as previously described [12].The NGS analysis generated 24.72 M raw reads and 24.18 M clean reads with a mean length of 150 nts after removing low-quality reads (Q30 > 95.45%, N50 = 369 bp). Finally, 55,114 contigs (>500bp) were obtained by de novo assembly, among which 748 contigs were mapped to the Potyvirus genome sequence.…”
Section: Full Textmentioning
confidence: 99%
“…To further identify the species of virus, the P. kingianum sample was sent to Ouyi Co., Ltd. (Shanghai, China) for next-generation sequencing (NGS), as previously described [12].The NGS analysis generated 24.72 M raw reads and 24.18 M clean reads with a mean length of 150 nts after removing low-quality reads (Q30 > 95.45%, N50 = 369 bp). Finally, 55,114 contigs (>500bp) were obtained by de novo assembly, among which 748 contigs were mapped to the Potyvirus genome sequence.…”
Section: Full Textmentioning
confidence: 99%