Molecular characterization, recombinant expression and bioactivity analysis of the interleukin-1β from the yellowfin sea bream, Acanthopagrus latus (Houttuyn)

Characterization of interleukin-1β as a proinflammatory cytokine in grass carp (Ctenopharyngodon idella)

“…Moreover, the baseline expression levels of gcIL-1b in various tissues measured in this study are in line with those observed in ayu [20]. However, these expression profiles are not in strict accordance with those reported in other fish [17,18]. The discrepancies in IL-1b expression profiles in different species likely are due to distinct immune cell systems, species variation, or differences in experimental systems.…”

“…To date, IL-1b has been identified in several teleost and cartilaginous fish species, including rainbow trout [9], common carp (Cyprinus carpio) [11], sea bass (Dicentrarchus labrax) [12], Atlantic salmon (Salmo salar) [13], channel catfish (Ictalurus punctatus) [14], Nile tilapia (Oreochromis niloticus) [15], Atlantic cod (Gadus morhua) [16], orange-spotted grouper (Epinephelus coioides) [17], yellowfin seabream (Acanthopagrus latus) [18], roughskin sculpin (Trachidermus fasciatus) [19], ayu (Plecoglossus altivelis) [20], Atlantic bluefin tuna (Thunnus thynnus) [21], and catshark (Scyliorhinus canicula) [22]. Several studies have shown that fish IL-1b proteins are involved in regulating the inflammatory response to bacterial or parasitic infection [23e26].…”

A macrophage migration inhibitory factor like oxidoreductase from pearl oyster Pinctada fucata involved in innate immune responses

“…When the OD 600 of bacteria concentration reached w0.6, isopropyl b-D-1-thiogalactopyranoside (IPTG) (MDBio) was added into LA medium at the final concentration of 0.8 mmol L À1 , then continued to incubate for 5 h. The resultant protein was electrophoreted on reducing sodium dodecyl sulfateepolyacrylamide gel electrophoresis (SDSePAGE), visualized with Coomassie brilliant blue R250. The proteins were transferred to physical vapor deposition (PVD) film, and western blot was performed [38], with antihis monoclonal antibody as the primary antibody and goat anti-rat IgG/HRP as secondary antibody (TIANGEN, Haidian District, Beijing, China). Finally, the film was colored using HRPeDAB coloration kit (TIANGEN).…”

Roles of Inflammatory Caspases during Processing of Zebrafish Interleukin-1β in Francisella noatunensis Infection

“…In addition, fish encode orthologs of inflammatory caspases (caspase 1 and caspase 5) that possess highly conserved catalytic domains, and functional studies have demonstrated that these caspase orthologs cleave caspase-1-and caspase-5-specific substrates and are inhibited by caspase-1-specific inhibitors (29,35). Although the accumulation of truncated forms of IL-1␤ of various lengths has been demonstrated following immune stimulus in carp (36), trout (15), and gilthead sea bream (20,29), the direct processing of nonmammalian IL-1␤ by a caspase 1 ortholog has not been addressed.…”