Molecular Characterization, Virulence Determinants, and Antimicrobial Resistance Profile of Methicillin-Resistant &lt;b&gt;&lt;i&gt;Staphylococcus aureus&lt;/i&gt;&lt;/b&gt; in the North of Iran; a High Prevalence of ST239-SCC&lt;b&gt;&lt;i&gt;mec&lt;/i&gt;&lt;/b&gt; III/t037 Clone

Hua, Ying; Mahmudiono, Trias; Alghazali, Tawfeeq; Abdelbasset, Walid Kamal; Khadivar, Parand; Rahimi, Somayeh; Amini, Abolfazl

doi:10.1159/000520482

Cited by 4 publications

(1 citation statement)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An increased incidence of levofloxacin-resistant Staphylococcus strains has been reported in ophthalmic infection [ 17 ], which limits its efficacy in the prevention of endophthalmitis after ocular surgery. In addition, methicillin-resistant Staphylococcus epidermidis (MRSE) strains have been found in more than 50% ophthalmic infections [ 18 ]. A wide spectrum of antibiotics has been developed for therapeutic use in coping with increased bacterial antibiotic resistance [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the effect of gentamicin in surgical perfusion solution on cataract postoperative endophthalmitis

Hou

Wang

et al. 2022

BMC Ophthalmol

View full text Add to dashboard Cite

Objective To evaluate the effect of gentamicin in surgical perfusion solution on endophthalmitis incidence after cataract surgery. Methods A retrospective analysis of endophthalmitis incidence was conducted in two groups of patients who underwent cataract surgery, with (Group B) or without gentamicin (Group A) in the surgical perfusion solution. Endophthalmitis incidence, the isolated pathogenic bacteria strains and their antibiotic sensitivity, and the drug-resistant genotype of the pathogens were examined. Results The incidence of endophthalmitis in patients of group A was 0.8‰. Thirteen pathogenic bacterial strains were isolated from the patient samples in group A, including 8 strains of Staphylococcus epidermidis, 1 Staphylococcus aureus, 1 Streptococcus pneumoniae, 1 Streptococcus bovis, 1 Enterococcus faecium and 1 Morganella sp. The incidence of endophthalmitis in group B patients was 0.2‰, which was significantly lower than that in group A (P<0.05). Five strains of pathogenic bacteria were successfully isolated, including 2 strains of Enterococcus faecium, 1 Enterococcus faecalis, 1 Staphylococcus epidermidis and 1 Staphylococcus aureus. There was no significant difference in the proportion of Staphylococcus strains in all isolated bacteria between the two groups (P > 0.05). However, the proportion of Enterococci isolated in group B samples was higher than that in group A (P < 0.05). There were more gentamicin-sensitive strains than levofloxacin-sensitive strains identified (P < 0.05). Interestingly, aminoglycoside-inactivating enzyme resistance gene was detected in Enterococcus strains. Conclusion Our data suggest that gentamicin-containing perfusion solution can reduce the incidence of postoperative endophthalmitis in cataract patients. However, the selective pressure imposed by gentamicin may facilitate the development of aminoglycoside-resistant Enterococcos strains.

show abstract

Section: Introductionmentioning

confidence: 99%

Evaluation of the effect of gentamicin in surgical perfusion solution on cataract postoperative endophthalmitis

Hou

Wang

et al. 2022

BMC Ophthalmol

View full text Add to dashboard Cite

show abstract

Widespread of the Vienna/Hungarian/Brazilian CC8-ST239-SCCmec III MRSA clone in patients hospitalized in the Tunisian Burn and Traumatology Center

Said

Thabet

Cheriet

et al. 2022

Letters in Applied Microbiology

View full text Add to dashboard Cite

The emergence and spread of methicillin-resistant Staphylococcus aureus (MRSA) in hospitals is a major global public health concern. The current study sought to characterize 25 MRSA clinical isolates collected in a Tunisian hospital from December 2015 to September 2016, with the genetic lineages, virulence factors, and antibiotic resistance mechanisms determined for these isolates. Three spa-types were detected: t037 (23 isolates), t932, and t2235 (one isolate each). Isolates were ascribed to agr I (n = 20), agr II (n = 1), with four nontypeable isolates. Depending on sequence type (ST), the 25 MRSA isolates were assigned to two clonal complexes (CC8 and CC5), with a predominance of the lineage ST239-CC8 (n = 24; 96%). All isolates belonging to CC8 had the SCCmec type III, while the unique CC5 isolate had SCCmec type IV. Antimicrobial susceptibility testing revealed high levels of resistance to aminoglycosides, tetracycline, ciprofloxacin and rifampicin for the majority of isolates belonging to the ST239-CC8 lineage. The ST149-CC5 isolate was susceptible to non-β-lactam antibiotics. One isolate harbored the tsst-1 gene (4%); however, lukS/LukF-PV, eta and etb genes were not detected. The MDR ST239-CC8 clone would seem to be widespread in this hospital. Therefore, a rigorous hygienic control system is urgently required.

show abstract

Molecular Characterization of Antimicrobial Resistance and Virulence Genotyping among Helicobacter pylori-Positive Dyspeptic Patients in North Iran

Kouhsari,

Roshandel,

Hosseinzadeh

et al. 2025

IDDT

View full text Add to dashboard Cite

Background: Iran has a relatively high prevalence of H. pylori, which correlates with high-risk areas for gastric cancer worldwide. Methods: Our study aimed to investigate the underlying genetic mechanisms associated with resistance to metronidazole (frxA, rdxA), clarithromycin (23S rRNA), tetracycline (16S rRNA), and fluoroquinolone (gyrA) in H. pylori-positive dyspeptic patients using PCR and sequencing. We further examined the potential correlation between resistance profiles and various virulence genotypes. Results: The rates of genetic mutations associated with resistance to metronidazole, fluoroquinolone, clarithromycin, and tetracycline were found to be 68%, 32.1%, 28.4%, and 11.1%, respectively. Well-documented multiple antibiotic resistance mutations were detected, such as rdxA and frxA (with missense and frameshift alterations), gyrA (Asp91, Asn87), 23S rRNA (A2142G, A2143G), and 16S rRNA (triple-base-pair substitutions AGA926-928→TTC). The cagA+ and vacA s1/m1 types were the predominant genotypes in our study. With the exception of metronidazole and tetracycline, no significant correlation was observed between the cagA+ and cagL+ genotypes and resistance-associated mutations. Conclusion: The prevalence of antibiotic resistance-associated mutations in H. pylori was remarkably high in this region, particularly to metronidazole, ciprofloxacin, and clarithromycin. By conducting a simultaneous screening of virulence and resistance genotypes, clinicians can make informed decisions regarding the appropriate therapeutic regimen to prevent the escalation of antibiotic resistance against H. pylori infection in this specific geographical location.

show abstract

Molecular Characterization, Virulence Determinants, and Antimicrobial Resistance Profile of Methicillin-Resistant <b><i>Staphylococcus aureus</i></b> in the North of Iran; a High Prevalence of ST239-SCC<b><i>mec</i></b> III/t037 Clone

Cited by 4 publications

References 51 publications

Evaluation of the effect of gentamicin in surgical perfusion solution on cataract postoperative endophthalmitis

Evaluation of the effect of gentamicin in surgical perfusion solution on cataract postoperative endophthalmitis

Widespread of the Vienna/Hungarian/Brazilian CC8-ST239-SCCmec III MRSA clone in patients hospitalized in the Tunisian Burn and Traumatology Center

Molecular Characterization of Antimicrobial Resistance and Virulence Genotyping among Helicobacter pylori-Positive Dyspeptic Patients in North Iran

Contact Info

Product

Resources

About