Molecular characterizations of antibiotic resistance, biofilm formation, and virulence determinants of <i>Pseudomonas aeruginosa</i> isolated from burn wound infection

Ghasemian, Shirin; Karami‐Zarandi, Morteza; Heidari, Hamid; Khoshnood, Saeed; Kouhsari, Ebrahim; Ghafourian, Sobhan; Maleki, Abbas; Kazemian, Hossein

doi:10.1002/jcla.24850

Cited by 13 publications

(6 citation statements)

References 43 publications

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“…In an investigation conducted by Banar et al [9] investigated 55/ 57 (96.5%) strains of P. aeruginosa were www.minarjournal.com characterize by their capacity to produce biofilms and found 30.9%, 47.3%, and 21.8% of these isolates formed strong, moderate and weak biofilm layers, respectively. Ghasemian et al [23] also reported strong, medium and weak biofilm production of 42.5%, 35% and 22.5%, respectively. In this research, it was evident that there was a statistically significant association between MBLs and biofilms formation and these findings are in line with those of Heydari and Eftekhar [18] , Ghasemian et al [23] , and Singhai et al [24] .…”

Section: Discussionmentioning

confidence: 93%

Detection of Biofilm Production Among MBLS-Producing Pseudomonas Aeruginosa Carrying Algd, Pelf, PSLD Genes Isolated From Burn Center in Al-Najaf Governorate, Iraq

K. ALKHUDHAIRY,

M. RASOOL

2023

MINAR Journal

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Background: Pseudomonas aeruginosa is a worldwide cause of chronic and acute infections of burn wounds. Aim of study: Detection of algD, pelF, and pslD genes encoding biofilm formation among isolates of Pseudomonas aeruginosa that producing metallo β-lactamases. Materials and Methods: 93 isolates of Pseudomonas aeruginosa were isolated from 362 inpatients with infected burn wounds at the burn center in Al-Najaf Governorate, Iraq, from September 2022 to February 2023. Results: Pseudomonas aeruginosa isolates were identified microscopically, phenotypically, culturally, and biochemically then by using the double-disc synergy method to test their capacity to produce metallo β-lactamases. Out of the 25 MBLs-producers, 22 (88%) were biofilm-producing isolates when tested by tissue culture plate method, 4 (16%), 10 (40%), and 8 (32%) were, respectively, weak, medium, and strongly biofilm producers. 25 metallo β-lactamases-producers under study, which had previously been evaluated using biofilm phenotypic method, were subjected to the investigation of biofilm encoding genes by using polymerase chain reaction technique. This molecular technique detected 21 (84%) isolates carried single and multiple biofilm encoding genes, 10(40%) isolates had algD gene, 6 (24%) isolates harbored algD and pslD genes and 5(20%) isolates carried algD, pelF and pslD genes. The single pslD and pelF genes were not detected among test isolates. Conclusions: There was a high prevalence of biofilmproducers among metallo β-lactamases-producing P. aeruginosa isolates. In addition, a clear positive correlation was observed between the multiple genes encoding biofilm and the degree of biofilm production among metallo β-lactamases-producers and close association between metallo β-lactamases and biofilms production, which explains the prevalence of carbapenems resistance and biofilm-production in this study because biofilm provides the appropriate environment for antibiotic resistance in general and carbapenems in particular

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Section: Discussionmentioning

confidence: 93%

Detection of Biofilm Production Among MBLS-Producing Pseudomonas Aeruginosa Carrying Algd, Pelf, PSLD Genes Isolated From Burn Center in Al-Najaf Governorate, Iraq

K. ALKHUDHAIRY,

M. RASOOL

2023

MINAR Journal

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“…Infections by dangerous pathogens, such as Pseudomonas aeruginosa or Acinetobacter baumannii, delay patient recovery and can even lead to patient death [29]. This is partially due to an increased resistance to antibiotics which has been described in Pseudomonas aeruginosa and Acinetobacter baumanii in the context of burn wounds [30,31]. Wound infection, however, starts with colonization [32].…”

Section: Discussionmentioning

confidence: 99%

Comparative Clinical Study of Suprathel® and Jelonet® Wound Dressings in Burn Wound Healing after Enzymatic Debridement

Heitzmann,

Mossing,

Fuchs

et al. 2023

Biomedicines

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Following the enzymatic debridement of deep dermal burns, the choice of wound dressing is crucial for providing an adequate environment and suitable conditions for rapid wound healing. As Suprathel® and fatty gauze (Jelonet®) are the most commonly used dressings in burn centers, the aim of this study is to compare Suprathel® and Jelonet® in the treatment of deep dermal burns after enzymatic debridement with respect to wound healing, patient comfort, and pain. A total of 23 patients with deep dermal burns of the hand or foot (mean total body surface area of 4.31%) were included in this prospective, unicentric, open, comparative, and intra-individual clinical study. After enzymatic debridement, wounds were divided into two areas: one was treated with Suprathel® and the other with Jelonet®. Suprathel® remained on the wounds without dressing changes while Jelonet® was regularly changed. Wound healing, infection, bleeding, exudation, time for dressing changes, and pain were documented (from days 2 to 48) after injury. Satisfactory results were obtained in 22 cases; only one patient had to undergo a second debridement followed by skin grafting. No significant difference in time to final wound healing could be observed (18–19 d). Patients reported significantly less pain during the dressing changes for Suprathel® compared to Jelonet®. Furthermore, the wound areas treated with Suprathel® showed significantly less exudation and bleeding. Wound infections rarely occurred in both groups. In conclusion, the authors found that both wound dressings could be used to achieve safe and rapid wound healing after the enzymatic debridement of deep dermal burns of the hands and feet. However, compared to Jelonet®, Suprathel® showed superior results in terms of patient comfort and pain reduction.

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“…investigated the antibacterial potential. With a blade, cuts were made that later became infected with Pseudomonas aeruginosa strains [27]. The effects of chamomile and tetracycline ointments were then compared.…”

Section: Chamomilla Anti-microbial Activitymentioning

confidence: 99%

The Immunological Significance of Medicinal Plants in Disease Control and Prevention in Humans

Alajeeli,

Al-Karawi,

Ali

2023

AJMS

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This abstract provides a review highlighting the significant role of medicinal plants in the treatment of various diseases globally. To explore this, a questionnaire was conducted in Baghdad to inquire whether individuals resorted to using medicinal plants when experiencing illness. The objective was to gain insights into the efficacy of medicinal plants when used as a form of treatment. Findings from studies conducted worldwide consistently indicate that medicinal plants are widely utilized due to their positive outcomes and cost-effectiveness compared to chemical drugs. Furthermore, ongoing research on the effects of medicinal plants has resulted in the development of novel therapies for numerous chronic ailments, including Parkinson’s disease, diabetes, hypertension, peptic ulcers, IBS syndrome, and other acute and chronic conditions.

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Molecular characterizations of antibiotic resistance, biofilm formation, and virulence determinants of Pseudomonas aeruginosa isolated from burn wound infection

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 13 publications

References 43 publications

Detection of Biofilm Production Among MBLS-Producing Pseudomonas Aeruginosa Carrying Algd, Pelf, PSLD Genes Isolated From Burn Center in Al-Najaf Governorate, Iraq

Detection of Biofilm Production Among MBLS-Producing Pseudomonas Aeruginosa Carrying Algd, Pelf, PSLD Genes Isolated From Burn Center in Al-Najaf Governorate, Iraq

Comparative Clinical Study of Suprathel® and Jelonet® Wound Dressings in Burn Wound Healing after Enzymatic Debridement

The Immunological Significance of Medicinal Plants in Disease Control and Prevention in Humans

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