1988
DOI: 10.1128/mcb.8.4.1504
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Molecular cloning and analysis of the regulation of cys-14+, a structural gene of the sulfur regulatory circuit of Neurospora crassa.

Abstract: The cys-14+ gene encodes sulfate permease II, which is primarily expressed in mycelia. cys-14+ is one of a set of sulfur-related structural genes under the control of cys-3+ and scon+, the regulatory genes of the sulfur control circuit. We have cloned cys-14+ from a cosmid library of Neurospora crassa DNA. A restriction fragment length polymorphism analysis showed that this clone maps to the region of chromosome IV corresponding to the cys-14+ locus. Northern blot analyses were used to examine the regulated ex… Show more

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Cited by 39 publications
(29 citation statements)
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“…In either case, the effect of the regulatory mutant was to increase ars-l+ message content. It is of interest that cys-14+ (sulfate permease) is affected similarly by cys-3 and sconc with regard to message content (19).…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…In either case, the effect of the regulatory mutant was to increase ars-l+ message content. It is of interest that cys-14+ (sulfate permease) is affected similarly by cys-3 and sconc with regard to message content (19).…”
Section: Resultsmentioning
confidence: 99%
“…The structural genes involved encode for arylsulfatase, choline sulfatase, choline sulfate permease, methionine permease, sulfate permeases I and II, and an extracellular protease. None of the known genes in the sulfur regulatory circuit are linked (14,19,23,36).…”
mentioning
confidence: 99%
“…Changes in mRNA levels, induced by sulfate starvation, ct,rresponded with changes of the transportation activities determined physiologically [4][5][6][7]. In the filamentous fungus, A eurospora crassa, expression of the cys14 gene encoding sulf~ te permease II was activated by sulfur limitation [8,9]. The p, ~sitive acting regulatory protein, Cys3, which binds to speci iic sites of the 5'-untranslated region of cys14, was required fi,r its expression [9,10].…”
Section: Introductionmentioning
confidence: 99%
“…The structural gene for sulfate permease II, cys-14, has been cloned and shown to encode an mRNA of approximately 3 kilobases (kb) whose content is highly regulated by cys-3, by scon, and by the sulfur status of the celis (11). Thus, it appears that both of the regulatory genes as well as sulfur repression act at the level of transcription or at a closely related step such as mRNA processing or stability.…”
mentioning
confidence: 99%