Molecular cloning and binding analysis of polymeric immunoglobulin receptor in largemouth bass (Micropterus salmoides)

Selection of internal reference gene for quantitative real‐time PCR analysis of gene expression in largemouth bass ( Micropterus salmoides ) under different experimental conditions

“…In salinity stimulation experiment, the β-actin was more applicable to the internal reference gene in gene expression analysis of Oratosquilla oratoria (Liu et al, 2013) or Portunus trituberculatus (Wang, Ren, et al, 2020), while β-actin could not be used as an internal reference gene in gene expression analysis of Trachidermus fasciatus (Kuang et al, 2020) and Sinonovaula constricta (Chen et al, 2021). The EF1α had the best expression stability and was the most ideal reference gene for Nitraria tangutorum under salt stress (Wang, Duan, et al, 2020), but it could be considered to be an lactea var.…”

“…With the rapid development of artificial breeding and aquaculture technology, largemouth bass has become an economically important fish species in China (Bai et al, 2008). In recent years, the researches of largemouth bass have mainly focused on genetic diversity (Dong et al, 2019; Fan et al, 2012), nutrition demand (Chen et al, 2010; Gong et al, 2019), molecular immunity (Hoang et al, 2020; Yang et al, 2015; Yang et al, 2021) and disease prevention (Xia et al, 2018; Yi et al, 2020). Therefore, the analysis of gene expression plays an important role in revealing the function and regulatory mechanisms of key target genes for largemouth bass.…”

“…, nutrition demand (Chen et al, 2010;Gong et al, 2019), molecular immunity (Hoang et al, 2020;Yang et al, 2015;Yang et al, 2021) and disease prevention (Xia et al, 2018;Yi et al, 2020).…”

Molecular Characterization of Immunoglobulin M (IgM) and Polymeric Immunoglobulin Receptor (pIgR) and Expression Response to Vibrio harveyi Challenge in Leopard Coral Grouper (Plectropomus leopardus)

“…SC is free or bound to polymeric IgM (or IgA) and contributes as a microbial scavenger to protect the epithelial surface [19,20]. Previous studies reported the gene sequences of pIgR in fugu (Takifugu rubripes) [21], common carp [17], orange-spotted grouper (Epinephelus coioides) [22], tlantic salmon (Salmo salar) [23], olive founder [24], turbot [25], sea bass (Lateolabrax japonicus) [26], crucian carp (Carassius auratus) [27], dojo loach (Misgurnus anguillicaudatus) [28], grass carp (Ctenopharyngodon idellus) [29], half-smooth tongue sole (Cynoglossus semilaevis) [30], and largemouth bass (Micropterus salmoides) [31]. Previously written articles demonstrated that the pIgR transported tetrameric IgM into the skin mucus in fugu and oliver founder [18,21].…”

Functional Characterization of Largemouth Bass (Micropterus salmoides) Soluble FcγR Homolog in Response to Bacterial Infection

“…Differently from mammals, Igs in teleost only four types (IgM, IgD, IgZ/IgT, IgM-IgZ chimera) [12], of which IgM was discovered firstly [13]. To date, polymeric immunoglobulin receptor (pIgR), one of the FcRs, in grass carp (Ctenopharyngodon idellus) [14], flounder (Paralichthys olivaceus) [15], Nile tilapia (Oreochromis niloticus) [16], ballan wrasse (Labrus bergylta) [17] and largemouth bass (Micropterus salmoides) [18] have been reported that could bind to IgM. However, regarding classical IgM-binding FcRs homologs, it has only been identified in FcγRI of ayu (Plecoglossus altivelis) [19] and FcRI of channel catfish (Ictalurus punctatus) [2].…”