Molecular cloning and characterization of the rubber elongation factor gene and its promoter sequence from rubber tree (Hevea brasiliensis): A gene involved in rubber biosynthesis

Venkatachalam, P.; Thulaseedharan, A.

doi:10.1016/j.plantsci.2006.05.009

Cited by 32 publications

(25 citation statements)

References 30 publications

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“…The diversity of isoprenoid compounds in plants suggests that these compounds occur in multibranched isoprenoid pathways (Bach, 1995). The pathway for rubber biosynthesis in H. brasiliensis is different from the pathway(s) leading to biosynthesis of other isoprenoid compounds (Suwanmanee et al, 2002;Priya et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning and characterization of a 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 (hmgr1) gene from rubber tree (Hevea brasiliensis Muell. Arg.): A key gene involved in isoprenoid biosynthesis

Venkatachalam

Rout

Rekha

et al. 2009

Physiol Mol Biol Plants

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Natural rubber (cis-1,4-polyisoprene) is a secondary metabolite produced in the laticiferous tissue of Hevea tree. Mevalonate synthesis, which is the first step in isoprenoid biosynthesis, is catalyzed by the enzyme 3-hydroxy-3-methylglutarylcoenzyme A reductase 1 (hmgr1). We have cloned and characterized a full-length cDNA as well as genomic DNA for hmgr1 gene from an elite Indian rubber clone (RRII 105). The nucleotide sequence of the genomic clone comprises 4 exons and 3 introns, giving a total length of 2440 bp. The sequences of 42 bp 5' UTR and 69 bp of the 3' UTR were also determined. The hmgr1 cDNA contained an open reading frame of 1838 bp coding for 575 amino acid protein with a theoretical pI value of 6.6 and the calculated protein M W was 61.6 kDa. The deduced amino acid sequence showed high identity with other plant hmgr1 sequences. The amino acid sequence of the Hevea hmgr1 revealed several motifs which are highly conserved and common to the other plant species. These sequence conservations suggest a strong evolutionary pressure to maintain amino acid residues at specific positions, indicating that the conserved motifs might play important roles in the structural and/or catalytic properties of the enzyme. Southern blot analysis of genomic DNA from Hevea probed with a genomic fragment indicated that there were at least three isoforms of hmgr in Hevea. This result reveals that hmgr1 is one of the members of a small gene family. (Northern blot analysis showed that hmgr1 mRNA transcripts were noticed in all tissues - latex, leaf, immature leaf, and seedlings), however, the abundance of transcript level was higher in latex cells. As one step towards a better understanding of the role that this enzyme plays in coordinating isoprenoid biosynthesis in plants, hmgr1 cDNA was over expressed in transgenic Arabidopsis plants. Transgenic plants were morphologically distinguishable from control wild-type plants and an increased expression level of hmgr1 mRNA was detected. These data suggest that hmgr1 gene expression is playing an important role in isoprenoid biosynthesis.

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Section: Discussionmentioning

confidence: 99%

Molecular cloning and characterization of a 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 (hmgr1) gene from rubber tree (Hevea brasiliensis Muell. Arg.): A key gene involved in isoprenoid biosynthesis

Venkatachalam

Rout

Rekha

et al. 2009

Physiol Mol Biol Plants

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“…Human serum albumin protein was expressed in transgenic Hevea plants by Arokiaraj et al ( 2002 ) . To characterize tissue-speci fi c promoters derived from latex biosynthesis genes, transgenic approaches were adopted by Priya et al ( 2006 ) . They cloned and characterized promoter sequence of the rubber elongation factor gene.…”

Section: Transgenesismentioning

confidence: 99%

Genomics of Hevea Rubber

Saha

Priyadarshan

2012

Genomics of Tree Crops

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Hevea brasiliensis is the most recent domesticated tree species from Amazonian rain forest producing latex of commercial utility. Major hurdles for genetic improvement of rubber tree were attributed to its perennial nature, long juvenile period, and a narrow genetic base. Further, the limited availability of Hevea genomic resources/information is another impediment to genomics-assisted crop improvement. Improvement of rubber tree in terms of latex production through breeding was the major focus of the scienti fi c community dealing with the crop. Due to unidirectional selection for yield, other secondary attributes of rubber plants were lost during the process of developing high-yielding clones.

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“…For initiation of rubber biosynthesis a priming allylic diphosphate is needed (Cornish, 2001a). IPP is isomerized to DMAPP by IPP-isomerase and is used as a substrate by transprenyltransferase (TPT) to synthesize an allylic initiator molecule (Priya et al, 2006;Koyama et al, 1996). It could be shown that initiation of rubber biosynthesis is most efficient with farnesyl diphosphate (FPP) in H. brasiliensis, Ficus elastica and P. argentatum in in vitro experiments (Xie et al, 2008).…”

Section: Rubber Biosynthesismentioning

confidence: 99%