Molecular cloning and characterization of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (CaHDR) from Camptotheca acuminata and its functional identification in Escherichia coli

Wang, Qian; Pi, Yan; Hou, Rong; Jiang, Keji; Huang, Zheng-Hai; Hsieh, M. T.; Sun, Xiaoping; Tang, Kexuan

doi:10.5483/bmbrep.2008.41.2.112

Cited by 20 publications

(11 citation statements)

References 29 publications

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“…However, it has been suggested that 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) and, recently, plant HDR genes may have a cyanobacterial origin (Lange et al, 2000;GuevaraGarcía et al, 2005;Wang et al, 2008). Therefore our data, which included green algae in phylogenetic analyses of HDR sequences for the first time, supports an endosymbiotic origin with a cyanobacterial ancestry for eukaryotic HDR genes.…”

Section: Phylogenetic Analysissupporting

confidence: 83%

Molecular and functional characterization of a cDNA encoding 4-hydroxy-3-methylbut-2-enyl diphosphate reductase from Dunaliella salina

Ramos

Marques

Rodrigues

et al. 2009

Journal of Plant Physiology

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Section: Phylogenetic Analysissupporting

confidence: 83%

Molecular and functional characterization of a cDNA encoding 4-hydroxy-3-methylbut-2-enyl diphosphate reductase from Dunaliella salina

Ramos

Marques

Rodrigues

et al. 2009

Journal of Plant Physiology

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“…annua plants were grown on MS medium at 25 ± 1°C, with a 16/8 h light/dark cycle, for two weeks. They were then transferred onto Petri dishes lined with moist filter paper and sprayed with either 100 μM GA 3 or 100 µM MeJA (Wang et al, 2008;Lu et al, 2012). For gene expression analysis, plants were selected randomly at different time points before treatment (0 h) and after treatment (1, 3, 6, 12, and 24 h).…”

Section: Hormone Treatmentsmentioning

confidence: 99%

Cloning and characterization of DELLA genes in Artemisia annua

Shen

Cui

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Gibberellins (GA) are some of the most important phytohormones involved in plant development. DELLA proteins are negative regulators of GA signaling in many plants. In this study, the full-length cDNA sequences of three DELLA genes were cloned from Artemisia annua. Phylogenetic analysis revealed that AaDELLA1 and AaDELLA2 were located in the same cluster, but AaDELLA3 was not. Subcellular localization analysis suggested that AaDELLAs can be targeted to the nucleus and/or cytoplasm. Real-time PCR indicated that all three AaDELLA genes exhibited the highest expression in seeds. Expression of all AaDELLA genes was enhanced by exogenous MeJA treatment but inhibited by GA 3 treatment. Yeast two-hybrid assay showed that AaDELLAs could interact with basic helix-loop-helix transcription factor AaMYC2, suggesting that GA and JA signaling may be involved in cross-talk via DELLA and MYC2 interaction in A. annua.

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“…So far, many CPT biosynthesis pathway genes were identified before strictosamide, such as CaTDC1, CaTDC2, CaG10H, CaHGO, CaSLS, CaSTR, CaSGD and genes involved in MVA and MEP pathways (López-Meyer and Nessler 1997;Sun et al 2011;Valletta et al 2010;Lu and McKnight 1999;Pan et al 2008;Kai et al 2013;Burnett et al 1993;Maldonado-Mendoza et al 1997;Wang et al 2008;Yao et al 2008;Lu et al 2005). Unfortunately, genes involved in the pathway between strictosamide and CPT, which was responsible for important later specific steps to form CPTs, still remained to be identified.…”

Section: Introductionmentioning

confidence: 95%

Application of virus-induced gene silencing approach in Camptotheca acuminata

Jin

Yan

Chang

et al. 2016

Plant Cell Tiss Organ Cult

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Camptotheca acuminata is the major plant for the production of camptothecin (CPT), an important anticancer drug used for the treatment of various cancers throughout the world. The low accumulation of CPT in plants limited its supply in the market and led to urgent need of promoting its accumulation by means of plant metabolic engineering, which relied on deep understanding of CPT biosynthesis pathway. However, missing of most of pathway genes restricted the attempts for regulating CPT biosynthesis. To unveil the CPT biosynthesis pathway, many efforts have been done for pathway gene identification and the application of large-scale RNA-sequencing technique accelerated screening of candidate genes which could be involved in CPT biosynthesis. To identify the function of these candidate genes in planta, it needs to develop an effective approach, such as virus-induced gene silencing (VIGS) in C. acuminata. In this work, a Tobacco Rattle Virus-based VIGS method was developed and the application of this method successfully silenced the expression of four known genes involved in the early steps of CPT biosynthesis and resulted in clear decrease of CPT and 10-hydroxycamptothecin (10-HCPT) accumulation. This VIGS approach could be further applied to functional identification of candidate genes to elucidate CPT biosynthesis in C. acuminata.

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Molecular cloning and characterization of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (CaHDR) from Camptotheca acuminata and its functional identification in Escherichia coli

Cited by 20 publications

References 29 publications

Molecular and functional characterization of a cDNA encoding 4-hydroxy-3-methylbut-2-enyl diphosphate reductase from Dunaliella salina

Molecular and functional characterization of a cDNA encoding 4-hydroxy-3-methylbut-2-enyl diphosphate reductase from Dunaliella salina

Cloning and characterization of DELLA genes in Artemisia annua

Application of virus-induced gene silencing approach in Camptotheca acuminata

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