Molecular cloning and characterization of P5CS gene from Jatropha curcas L.

Zhuang,

doi:10.5897/ajb11.2072

Cited by 10 publications

(8 citation statements)

References 23 publications

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“…One key gene identified in the QTL was delta-1-pyrroline-5-carboxylate synthase ( P5CS ) (Table 4 ). P5CS catalyses the reduction of Glu to delta1-pyrroline-5-carboxylate (P5C), a critical step in the de novo biosynthesis of Pro, ornithine, and arginine [ 60 ], and is thus a vital enzyme in Glu and other AA metabolism. Two genes related to glycometabolism were identified in qTau_2 and qTau_4, respectively (Table 4 ).…”

Section: Resultsmentioning

confidence: 99%

Construction of a high-density genetic map and fine QTL mapping for growth and nutritional traits of Crassostrea gigas

Wang

Song

et al. 2018

BMC Genomics

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BackgroundBoth growth and nutritional traits are important economic traits of Crassostrea gigas (C. gigas) in industry. But few work has been done to study the genetic architecture of nutritional traits of the oyster. In this study, we constructed a high-density genetic map of C. gigas to help assemble the genome sequence onto chromosomes, meanwhile explore the genetic basis for nutritional traits via quantitative trait loci (QTL) mapping.ResultsThe constructed genetic map contained 5024 evenly distributed markers, with an average marker interval of 0.68 cM, thus representing the densest genetic map produced for the oyster. According to the high collinearity between the consensus map and the oyster genome, 1574 scaffold (about 70%) of the genome sequence of C. gigas were successfully anchored to 10 linkage groups (LGs) of the consensus map. Using this high-qualified genetic map, we then conducted QTL analysis for growth and nutritional traits, the latter of which includes glycogen, amino acid (AA), and fatty acid (FA). Overall, 41 QTLs were detected for 17 traits. In addition, six candidate genes identified in the QTL interval showed significant correlation with the traits on transcriptional levels. These genes include growth-related genes AMY and BMP1, AA metabolism related genes PLSCR and GR, and FA metabolism regulation genes DYRK and ADAMTS.ConclusionUsing the constructed high-qualified linkage map, this study not only assembled nearly 70% of the oyster genome sequence onto chromosomes, but also identified valuable markers and candidate genes for growth and nutritional traits, especially for AA and FA that undergone few studies before. These findings will facilitate genome assembly and molecular breeding of important economic traits in C. gigas.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4996-z) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Construction of a high-density genetic map and fine QTL mapping for growth and nutritional traits of Crassostrea gigas

Wang

Song

et al. 2018

BMC Genomics

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“…Induction of transcript expression of gene involved in Proline biosynthesis (Δ1-pyrroline-5-carboxylate synthase- P5CS ) has been reported in response to abiotic stresses (Neffar et al 2011; Zhuang et al 2011). Further, over expression of the gene has resulted in enhanced Proline accumulation and presumably, the stress tolerance (Vendruscolo et al 2007; Sharma et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Cold tolerance in Osmotin transgenic tomato (Solanum lycopersicum L.) is associated with modulation in transcript abundance of stress responsive genes

et al. 2013

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Our containment trials have established cold tolerance in Nicotiana tabacum osmotin (Nt Osm) transgenic tomato (Solanum lycopersicum L. cv. Pusa Ruby). Though, the stress tolerance mechanisms have been studied at physio-biochemical levels, molecular mechanisms underlying the tolerant response are still not well studied. Therefore, quantitative transcript expression of Osmotin and other stress responsive genes (CBF1, P5CS and APX) was studied in response to cold (4°C; 2 and 24 h) treatment in the transgenic and wild type tomato plants. The expression analysis revealed differential transcript regulation in the transgenic and wild type plants on the cold exposure. In general, the genes were either earlier induced or the extent of fold change in transcript expression over the respective untreated controls was higher in transgenic than in the wild type plants on cold exposure. The transcript expression data also supported the metabolite analysis on free Proline and ascorbate content. The results thus suggest that constitutive over expression of the Osmotin modulate transcript abundance and functional expression products of the other stress responsive genes thereby, imparting cold tolerance in the transgenic tomato plants.Electronic supplementary materialThe online version of this article (doi:10.1186/2193-1801-2-117) contains supplementary material, which is available to authorized users.

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“…14 For integration into device architectures, surface-anchored metal-organic frameworks (SurMOFs) can be deposited directly onto electrodes. [15][16][17][18][19][20][21][22][23][24] Metal-organic coordination offers control over the layer-by-layer (LbL) assembly of metal-organic multilayers with sequential deposition of metal and organic components on a functionalized surface, permitting the atomic control of film structure and composition. [25][26][27][28][29][30][31][32][33] An advantage of this deposition technique is the ability to fabricate highly ordered films that can be preferentially oriented to facilitate ionic conduction.…”

Section: Graphical Abstract Introductionmentioning

confidence: 99%

Conformal Ultrathin Film Metal–Organic Framework Analogues: Characterization of Growth, Porosity, and Electronic Transport

et al. 2019

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Thin-film formation and transport properties of two copper-paddlewheel metal-organic framework (MOF) -based systems (MOF-14 and MOF-399) are investigated for their potential integration into electrochemical device architectures. Thin-film analogs of these two systems are fabricated by the sequential, alternating, solution-phase deposition of the inorganic and organic ligand precursors that result in conformal films via van der Merwe-like growth. Atomic force microscopy reveals smooth film morphologies with surface roughnesses determined by the underlying substrates and linear film growth of 1.4 and 2.2 nm per layer for the MOF-14 and MOF-399 systems, respectively. Electrochemical impedance spectroscopy is used to evaluate the electronic transport properties of the thin films, finding that the MOF-14 analog films demonstrate low electronic conductivity, while MOF-399 analog films are electronically insulating. The intrinsic porosities of these ultrathin MOF analog films are confirmed by cyclic voltammetry redox probe characterization using ferrocene. Larger peak currents are observed for MOF-399 analog films compared to MOF-14 analog films, which is consistent with the larger pores of MOF-399. The layer-by-layer deposition of these systems provides a promising route to incorporate MOFs as thin films with nanoscale thickness control and low surface roughness for electrochemical devices.

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Molecular cloning and characterization of P5CS gene from Jatropha curcas L.

Cited by 10 publications

References 23 publications

Construction of a high-density genetic map and fine QTL mapping for growth and nutritional traits of Crassostrea gigas

Construction of a high-density genetic map and fine QTL mapping for growth and nutritional traits of Crassostrea gigas

Cold tolerance in Osmotin transgenic tomato (Solanum lycopersicum L.) is associated with modulation in transcript abundance of stress responsive genes

Conformal Ultrathin Film Metal–Organic Framework Analogues: Characterization of Growth, Porosity, and Electronic Transport

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