Molecular cloning and characterization of chemokine-like factor super family member 1 (CKLFSF1), a novel human gene with at least 23 alternative splicing isoforms in testis tissue

Wang, Lu; Wu, Chunxiao; Zheng, Ying; Qiu, Xiaoyan; Wang, Li; Fan, Hongyuan; Han, Wenling; Lv, Bingfeng; Wang, Ying; Zhu, Xiaohui; Xu, Mingxu; Ding, Peiguo; Cheng, Shanhong; Zhang, Yingmei; Song, Quansheng; Ma, Dalong

doi:10.1016/j.biocel.2003.11.017

Cited by 33 publications

(18 citation statements)

References 20 publications

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“…They code for CMTM1 (146-fold increase; ref. 34), whose role is still unclear, and CMKLR1/chemR23 (120-fold increase). The latter is a multifuctional receptor, usually highly expressed by monocyte-derived human macrophages and immature plasmacytoid DCs (35), leading to their chemerin-mediated migration.…”

Section: Discussionmentioning

confidence: 99%

Interleukin-30 Expression in Prostate Cancer and Its Draining Lymph Nodes Correlates with Advanced Grade and Stage

Meo

Airoldi

Sorrentino

et al. 2014

Clinical Cancer Research

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Purpose: The interleukin (IL)-27 cytokine subunit p28, also called IL-30, has been recognized as a novel immunoregulatory mediator endowed with its own functions. These are currently the subject of discussion in immunology, but completely unexplored in cancer biology. We set out to investigate the role of IL-30 in prostate carcinogenesis and its effects on human prostate cancer (hPCa) cells.Experimental Design: IL-30 expression, as visualized by immunohistochemistry and real-time reverse transcriptase PCR on prostate and draining lymph nodes from 125 patients with prostate cancer, was correlated with clinicopathologic data. IL-30 regulation of hPCa cell viability and expression of selected gene clusters was tested by flow cytometry and PCR array.Results: IL-30, absent in normal prostatic epithelia, was expressed by cancerous epithelia with Gleason ! 7% of 21.3% of prostate cancer stage I to III and 40.9% of prostate cancer stage IV. IL-30 expression by tumor infiltrating leukocytes (T-ILK) was higher in stage IV that in stage I to III prostate cancer (P ¼ 0.0006) or in control tissue (P ¼ 0.0011). IL-30 expression in prostate draining lymph nodes (LN)-ILK was higher in stage IV than in stage I to III prostate cancer (P ¼ 0.0031) or in control nodes (P ¼ 0.0023). The main IL-30 sources were identified as CD68 þ macrophages, CD33 þ

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Section: Discussionmentioning

confidence: 99%

Interleukin-30 Expression in Prostate Cancer and Its Draining Lymph Nodes Correlates with Advanced Grade and Stage

Meo

Airoldi

Sorrentino

et al. 2014

Clinical Cancer Research

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“…Of the 10 most highly expressed germcell genes listed in Supplemental Table S3, eight (Gsg1, Oaz3, Odf2, Smcp, Odf1, Tnp1, Crisp2, and Tnp2) are all well-known male germ cell-specific genes, and Hspb9 has also been shown to be germ-cell specific [57]. Cmtm2a (previously known as Cklfsf1) is highly expressed in spermatocytes [58], and the data of the current study now indicate it to be germ-cell specific.…”

Section: Distinguishing Germ Cell-specific and Somatic Cell-enriched mentioning

confidence: 98%

Changes in Gene Expression in Somatic Cells of Rat Testes Resulting from Hormonal Modulation and Radiation-Induced Germ Cell Depletion1

Zhou

Bolden-Tiller

Shetty

et al. 2010

Biology of Reproduction

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Although gonadotropins and androgen are required for normal spermatogenesis and both testosterone and follicle-stimulating hormone (FSH) are responsible for the inhibition of spermatogonial differentiation that occurs in irradiated rats, it has been difficult to identify the specific genes involved. To study specific hormonally regulated changes in somatic cell gene expression in the testis that may be involved in these processes, without the complication of changing populations of germ cells, we used irradiated LBNF(1) rats, the testes of which contain almost exclusively somatic cells except for a few type A spermatogonia. Three different groups of these rats were treated with various combinations of gonadotropin-releasing hormone antagonist, an androgen receptor antagonist (flutamide), testosterone, and FSH, and we compared the gene expression levels 2 wk later to those of irradiated-only rats by microarray analysis. By dividing the gene expression patterns into three major patterns and 11 subpatterns, we successfully distinguished, in a single study, the genes that were specifically regulated by testosterone, by luteinizing hormone (LH), and by FSH from the large number of genes that were not hormonally regulated in the testis. We found that hormones produced more dramatic upregulation than downregulation of gene expression: Testosterone had the strongest upregulatory effect, LH had a modest but appreciable upregulatory effect, and FSH had a minor upregulatory effect. We also separately identified the somatic cell genes that were chronically upregulated by irradiation. Thus, the present study identified gene expression changes that may be responsible for hormonal action on somatic cells to support normal spermatogenesis and the hormone-mediated block in spermatogonial development after irradiation.

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“…The transmembrane-type chemokine-like factor (CKLF) and the protein family CMTM (CKLF-like MARVEL transmembrane domain containing) 1-8 constitute gene clusters in humans and mice. In this cluster, the testis-specific enriched genes Cmtm1 and Cmtm2 were identified in humans, and Cmtm1, Cmtm2a, and Cmtm2b were present in mice (Li et al, 2006;Shi et al, 2005;Wang et al, 2004). To examine the physiological roles of these genes, Fujihara, Oji, Kojima-Kita, Larasati, and Ikawa (2018) generated CRISPR/Cas9-mediated mutant mouse strains.…”

Section: Testis-specific Serine Proteases (Prss3and Prss55)mentioning

confidence: 99%

An update of the regulatory factors of sperm migration from the uterus into the oviduct by genetically manipulated mice

Xiong

Wang

Shen

2019

Molecular Reproduction Devel

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Mammalian reproductive processes involve spermatogenesis, which occurs in the testis, and fertilization, which takes place in the female genital tract. Fertilization is a successive, multistep, and extremely complicated event that usually includes sperm survival in the uterus, sperm migration through the uterotubal junction (UTJ) and the oviduct, sperm penetration through the cumulus cell layer and the zona pellucida, and sperm–egg fusion. There may be a complex molecular mechanism to ensure that the above processes run smoothly. Previous studies have discovered essential factors for these fertilization steps through in vitro fertilization experiments. However, recent gene disruption approaches in mice have revealed that many of the factors previously described as important for fertilization are largely dispensable in gene‐knockout animals, and some previously unknown factors are emerging. As a result, the molecular mechanisms of fertilization, especially sperm migration from the uterus into the oviduct, have recently been revised by the emergence of genetically modified animals. In this review, we only focus on and update the essential genes for sperm migration through the UTJ and describe recent advances in our knowledge of the basis of mammalian sperm migration.

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Molecular cloning and characterization of chemokine-like factor super family member 1 (CKLFSF1), a novel human gene with at least 23 alternative splicing isoforms in testis tissue

Cited by 33 publications

References 20 publications

Interleukin-30 Expression in Prostate Cancer and Its Draining Lymph Nodes Correlates with Advanced Grade and Stage

Interleukin-30 Expression in Prostate Cancer and Its Draining Lymph Nodes Correlates with Advanced Grade and Stage

Changes in Gene Expression in Somatic Cells of Rat Testes Resulting from Hormonal Modulation and Radiation-Induced Germ Cell Depletion1

An update of the regulatory factors of sperm migration from the uterus into the oviduct by genetically manipulated mice

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