Molecular cloning and characterization of seven class III peroxidases induced by overexpression of the agrobacterial rolB gene in Rubia cordifolia transgenic callus cultures

Veremeichik, Galina N.; Shkryl, Yuri N.; Bulgakov, Victor P.; Авраменко, Т.В.; Zhuravlev, Yu. N.

doi:10.1007/s00299-011-1219-3

Cited by 22 publications

(12 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Another feature of rolB is that the gene strongly activates expression of the extracellular class III peroxidase genes and enhances total peroxidase activity in transformed cells [61]. This effect is dose-dependent and is observed in all rolBexpressing plant species studied.…”

Section: Ros Homeostasis and Expression Of Antioxidant Genesmentioning

confidence: 97%

Recent Advances in the Understanding of Agrobacterium rhizogenes-Derived Genes and Their Effects on Stress Resistance and Plant Metabolism

Bulgakov

Shkryl

Veremeichik

et al. 2013

Advances in Biochemical Engineering/Biotechnology

View full text Add to dashboard Cite

It is commonly accepted that the plant pathogens Agrobacterium rhizogenes and Agrobacterium tumefaciens, acting via their T-DNA oncogenes, disturb hormone metabolism or hormone perception pathways in plants, thereby attaining their aim of successful pathogenesis. In this work, we summarize recent data on the A. rhizogenes rolC and rolB oncogenes in comparison to the A. tumefaciens 6b oncogene with respect to their effects on the physiology of transformed cells. The newly discovered functions of the rol genes include the modulation of secondary metabolism, the modulation of levels of intracellular ROS and stress resistance of transformed cells, changed sucrose metabolism, and the inhibition of programmed cell death. We show that the rol genes do not have suppressive effects on plant innate immunity; rather, these genes activate plant defense reactions. The existence of not only the hormone-related mechanism of pathogenicity but also the defense-related mechanism of pathogenicity during plant-Agrobacterium interactions is suggested.

show abstract

Section: Ros Homeostasis and Expression Of Antioxidant Genesmentioning

confidence: 97%

Recent Advances in the Understanding of Agrobacterium rhizogenes-Derived Genes and Their Effects on Stress Resistance and Plant Metabolism

Bulgakov

Shkryl

Veremeichik

et al. 2013

Advances in Biochemical Engineering/Biotechnology

View full text Add to dashboard Cite

show abstract

“…Likewise, rolC diminished the rolB-induced high sensitivity to auxin in transformed cells (Maurel et al, 1991) and the severity of rolB-induced phenotypes (Capone et al, 1989;Vanaltvorst et al, 1992). Recently, a contrasting difference between the action of rolB and rolC on class III peroxidase gene expression has been demonstrated (Veremeichik et al, 2012). Although overexpression of a single rolB gene caused dramatic up-regulation of R. cordifolia class III peroxidase genes, the effect of the rolC gene on peroxidase transcript abundance was minimal.…”

Section: Combined Effect Of the Rolb And Rolc Genesmentioning

confidence: 99%

TherolBGene Suppresses Reactive Oxygen Species in Transformed Plant Cells through the Sustained Activation of Antioxidant Defense

et al. 2012

View full text Add to dashboard Cite

The rolB (for rooting locus of Agrobacterium rhizogenes) oncogene has previously been identified as a key player in the formation of hairy roots during the plant-A. rhizogenes interaction. In this study, using single-cell assays based on confocal microscopy, we demonstrated reduced levels of reactive oxygen species (ROS) in rolB-expressing Rubia cordifolia, Panax ginseng, and Arabidopsis (Arabidopsis thaliana) cells. The expression of rolB was sufficient to inhibit excessive elevations of ROS induced by paraquat, menadione, and light stress and prevent cell death induced by chronic oxidative stress. In rolB-expressing cells, we detected the enhanced expression of antioxidant genes encoding cytosolic ascorbate peroxidase, catalase, and superoxide dismutase. We conclude that, similar to pathogenic determinants in other pathogenic bacteria, rolB suppresses ROS and plays a role not only in cell differentiation but also in ROS metabolism.

show abstract

“…It was previously shown that the majority of antioxidant genes studied, including those encoding the Cu/Zn superoxide dismutases, catalases, ascorbate peroxidases and class III peroxidase genes, were coordinately upregulated in rolB-expressing cells. 5,19 RolB permanently sustains an active anti-oxidative environment in transformed cells and simultaneously maintains their normal redox balance, providing physiological conditions for adaptation to external oxidative stress. 5 While the cellular conditions leading to PCD induction are linked with an imbalance in redox status, 20 this function of RolB may be important for PCD prevention.…”

Section: -11mentioning

confidence: 99%

Can plant oncogenes inhibit programmed cell death? The rolB oncogene reduces apoptosis-like symptoms in transformed plant cells

Gorpenchenko

Aminin

Vereshchagina

et al. 2012

Plant Signaling & Behavior

View full text Add to dashboard Cite

The interplay between oncogene expression, ROS production and apoptosis has received a great deal of attention in animal cell biology. Few studies have focused on the role of plant oncogenes in these processes. Plant oncogenes are Agrobacterium T-DNA genes of the plast (RolB) family of eukaryotic regulators.1 During plant infection by the pathogen Agrobacterium rhizogenes, the rolA, rolB and rolC oncogenes are transferred from the bacteria into the plant genome, causing tumor formation and hairy root disease.2 The function of rolB is not restricted to root formation; the gene promotes de novo formation of floral and shoot meristems, induces parthenocarpy, causes a delay in pistil and anther development and modifies the balance between the proliferation of procambial cells and xylem differentiation. 3 The mechanism by which the RolB oncoprotein exerts such varied morphological changes remains unknown.Recently, involvement of the rolB and rolC plant oncogenes in ROS metabolism was revealed. 4,5 Both genes, when expressed in plant cells, decreased intracellular ROS level. The rolB gene acts by inducing upregulation of antioxidant genes, thereby permanently supporting an active anti-oxidative status of transformed cells.5 As ROS perturbations and apoptotic processes are tightly linked, we proposed involvement of the RolB protein in signaling networks regulating PCD in plants. The present investigation was aimed at testing this possibility.The rolB oncogene was previously identified as an important player in ROS metabolism in transformed plant cells. Numerous reports indicate a crucial role for animal oncogenes in apoptotic cell death. Whether plant oncogenes such as rolB can induce programmed cell death (PCD) in transformed plant cells is of particular importance. In this investigation, we used a single-cell assay based on confocal microscopy and fluorescent dyes capable of discriminating between apoptotic and necrotic cells. Our results indicate that the expression of rolB in plant cells was sufficient to decrease the proportion of apoptotic cells in steady-state conditions and diminish the rate of apoptotic cells during induced PCD. These data suggest that plant oncogenes, like animal oncogenes, may be involved in the processes mediating PCD. Can plant oncogenes inhibit programmed cell death?The rolB oncogene reduces apoptosis-like symptoms in transformed plant cells Necrosis, a rapid form of cell death, is characterized by cytoplasmic swelling, destruction of organelles and disruption of the plasma membrane. 6 Apoptosis is characterized by cell shrinkage, maintenance of organelle integrity and condensation and fragmentation of DNA.6 A distinct type of PCD called apoptosis was originally identified in single cells that were usually surrounded by healthy-looking neighbors. 6 In the present work, we used the terms "PCD" and "apoptosis" interchangeably. Early symptoms of apoptosis, such as nuclear chromatin condensation at the periphery of nucleus and around nucleoli, were studied. To distinguish between necrotic and...

show abstract

Molecular cloning and characterization of seven class III peroxidases induced by overexpression of the agrobacterial rolB gene in Rubia cordifolia transgenic callus cultures

Cited by 22 publications

References 49 publications

Recent Advances in the Understanding of Agrobacterium rhizogenes-Derived Genes and Their Effects on Stress Resistance and Plant Metabolism

Recent Advances in the Understanding of Agrobacterium rhizogenes-Derived Genes and Their Effects on Stress Resistance and Plant Metabolism

TherolBGene Suppresses Reactive Oxygen Species in Transformed Plant Cells through the Sustained Activation of Antioxidant Defense

Can plant oncogenes inhibit programmed cell death? The rolB oncogene reduces apoptosis-like symptoms in transformed plant cells

Contact Info

Product

Resources

About