Molecular cloning and characterization of six novel <scp>HMW</scp>‐<scp>GS</scp> genes from <i><scp>A</scp>egilops speltoides</i> and <i><scp>A</scp>egilops kotschyi</i>

Ma, Chao; Yang, Yang; Li, Xiaohui; Ge, Pei; Guo, Guangfang; Subburaj, Saminathan; Zeller, F. J.; Hsam, S. L. K.; Yan, Yueming

doi:10.1111/pbr.12046

Cited by 21 publications

(15 citation statements)

References 39 publications

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“…tauschii and Ae. cylindrica) and rye, primary structure conservation in HMW-GS was also noted (Jiang et al, 2010;Ma et al, 2013;Mackie et al, 1996;Wan et al, 2000). This suggests that these proteins play important roles in seed development and germination.…”

Section: Discussionmentioning

confidence: 82%

Characterization of novel high-molecular-weight glutenin subunits and their coding sequences in Aegilops markgrafii

Liu

Sun

et al. 2015

Journal of Cereal Science

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Section: Discussionmentioning

confidence: 82%

Characterization of novel high-molecular-weight glutenin subunits and their coding sequences in Aegilops markgrafii

Liu

Sun

et al. 2015

Journal of Cereal Science

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“…sharonensis also corresponded to the Glu1Bx7 of the Chinese Spring (Jiang et al 2012). Ma et al (2013) characterized five novel HMWGS from different accessions of Ae. kotschyi designated as AKx1*, AKx3*, AKx2.3*, AKy20* and AKy8* on the basis of their electrophoretic mobility.…”

Section: Sds-page Hmwgs Profile Of Selected Pollen Radiation Hybrids mentioning

confidence: 99%

Transfer of HMW glutenin subunits from Aegilops kotschyi to wheat through radiation hybridization

et al. 2016

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High molecular weight glutenin subunits (HMWGS) are responsible for dough elasticity and bread making quality of bread wheat. Related wild non-progenitor species, Aegilops kotschyi possesses higher molecular weight x and y glutenin subunits than the bread wheat cultivars. A wheat-Aegilops substitution line with 1U chromosome was used for the transfer of (HMWGS) of 1U to wheat by using pollen radiation hybridization approach. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiling showed different patterns of allelic variations with either the presence or absence of HMWGS, Glu-1A (1, null), Glu-1B (7, 7 ? 8, 17 ? 18) and Glu-1D (5 ? 10, 2 ? 12, null). The pollen irradiated wheat-Aegilops derivatives, B-56-1-4-2, B-56-1-4-3, B-14-1 and B-14-2 with Glu1Ux and 1Uy and absence or presence of some Glu-1A and Glu-1B HMWGS showed high micro SDS sedimentation test (MST) values while B-16-1 and B-16-2 had moderate MST values and high protein content. However, B-58-3 with transfer of Glu-1Ux ? 1Uy for Glu-1D showed very low MST values indicating that Glu1Ux ? 1Uy enhance MST value only in the presence of Glu1D HMWGS. The transfer/substitution of alien HMW-GS for Glu-1A and or Glu-1B loci only can lead to improved bread making quality of wheat.

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“…coli [2,8,10,13,14,32,34,36,42,43]. However, being removed signal sequence, the mRNA of HMW-GS gene Dx failed to be detected by Northern blotting [11].…”

Section: Effect Of Signal Sequence On the Transcription Level Of Exogmentioning

confidence: 99%

“…Heterologous expression is particularly important for storage protein structure-function studies. The expressed proteins can be used to determine the functional properties of single subunit by small-scale testing [4][5][6][7][8], and to identify the corresponding native subunit in the wheat endosperm [9][10][11][12][13][14]. In the past several decades, three main types of expression systems have been widely used for heterologous protein production, viz.…”

Section: Background and Introductionmentioning

confidence: 99%

High-level expression of LMW-GS and α-gliadin genes promoted by the expressed tag sequence of 5′ end in Escherichia coli

Wang

Gao

et al. 2015

Protein Expression and Purification

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractWheat storage protein genes, especially low molecular weight glutenin subunit (LMW-GS) and gliadin genes are difficult to be expressed in E. coli, mainly due to the presence of highly repetitive sequences. In order to establish a high efficiency expression system for these genes, five different expression plasmids combining with 2 9 genes, viz. 6 LMW-GS and 3 α-gliadin genes isolated from common wheat and related species, were studied for heterologous expression in E. coli. In this study, when an expressed tag sequence encoding signal peptide, His-S or GST-tag was fused to the 5'end of LMW-GS or gliadin gene as the leading sequence, all recombination genes could be stably expressed at a high level. On the contrast, as expected, the inserted genes encoding mature protein failed without an expressed tag sequence. This result indicated that using expressed tag sequences as leading sequences could promote LMW-GS and gliadin genes to be well expressed in E. coli. Further transcriptional analysis by quantitative real-time PCR (qRT-PCR) showed transcription levels of recombination genes (e.g. GST-Glutenin, His-S-Glutenin and SP*-His-Glutenin) were 4-fold to 33-fold higher than those of the LMW-GS genes, which suggested these expressed tag sequences might play an important role in stimulating transcription. The possible molecular mechanism under this phenomenon was discussed.

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Molecular cloning and characterization of six novel HMW‐GS genes from Aegilops speltoides and Aegilops kotschyi

Cited by 21 publications

References 39 publications

Characterization of novel high-molecular-weight glutenin subunits and their coding sequences in Aegilops markgrafii

Characterization of novel high-molecular-weight glutenin subunits and their coding sequences in Aegilops markgrafii

Transfer of HMW glutenin subunits from Aegilops kotschyi to wheat through radiation hybridization

High-level expression of LMW-GS and α-gliadin genes promoted by the expressed tag sequence of 5′ end in Escherichia coli

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