Molecular cloning and characterization of one member of 3β-hydroxy sterol glucosyltransferase gene family in Withania somnifera

Sharma, Lokendra; Madina, Bhaskara Reddy; Chaturvedi, Pankaj; Sangwan, Rajender S.; Tuli, Rakesh

doi:10.1016/j.abb.2007.01.024

Cited by 69 publications

(58 citation statements)

References 37 publications

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“…Differential expression of SGT gene was observed in different plant parts of W. somnifera. Elicitor-treated plants showed more SGT transcript accumulation than nonelicited plants (Sharma et al 2007). Thus, it was anticipated that reduced amount of withasteroid accumulation in cryptogein-cotransformed hairy roots should possess low levels of SGT transcript accumulation.…”

Section: Discussionmentioning

confidence: 95%

“…Withasteroids are glucosylated sterols (Mishra et al 2005). One member of 3β-hydroxy sterol glucosyltransferase (SGT) gene family was cloned recently from W. somnifera (Sharma et al 2007). Differential expression of SGT gene was observed in different plant parts of W. somnifera.…”

Section: Discussionmentioning

confidence: 99%

“…Most of the higher plant sterols possess b-OH group at C-3 (A-ring) and are transformed into their glycoconjugates by sterol glucosyltransferases (SGTs) (Sharma et al 2007). In plants, sterols are biosynthesized by mevalonate (Dewick 2001) and nonmevalonate pathways (Rohmer 1999).…”

Section: Discussionmentioning

confidence: 99%

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Metabolic shift from withasteroid formation to phenylpropanoid accumulation in cryptogein-cotransformed hairy roots of Withania somnifera (L.) Dunal

et al. 2014

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Cotransformed hairy roots containing a gene that encodes a fungal elicitor protein, β-cryptogein, were established in Withania somnifera, a medicinal plant widely used in Indian systems of medicine. To find out whether β-cryptogein protein endogenously elicits the pathway of withasteroid biosynthesis, withaferin A and withanolide A contents along with transcript accumulation of farnesyl pyrophosphate (FPP) synthase, 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMGR), and sterol glycosyltransferase (SGT) were analyzed in both cryptogein-cotransformed and normal hairy roots of W. somnifera. It was observed that the withaferin A and withanolide A contents were drastically higher in normal hairy roots than cryptogein-cotransformed ones. Similar trends were also observed on the levels of transcript accumulation. Subsequently, the enzyme activity of phenylalanine ammonia lyase (PAL), one of the key enzymes of phenylpropanoid pathway, was measured in both cryptogein-cotransformed and normal hairy roots of W. somnifera along with the levels of PAL transcript accumulation. Upliftment of PAL activity was observed in cryptogein-cotransformed hairy roots as compared to the normal ones, and the PAL expression also reflected a similar trend, i.e., enhanced expression in the cryptogein-cotransformed lines. Upliftment of wall-bound ferulic acid accumulation was also observed in the cryptogein-cotransformed lines, as compared to normal hairy root lines. Thus, the outcome of the above studies suggests a metabolic shift from withanolide accumulation to phenylpropanoid biosynthesis in cryptogein-cotransformed hairy roots of W. somnifera.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

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Metabolic shift from withasteroid formation to phenylpropanoid accumulation in cryptogein-cotransformed hairy roots of Withania somnifera (L.) Dunal

et al. 2014

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“…The variation in the dissipation of lindane between the non-vegetated treatments and vegetated treatments was principally due to the plant effect. W. somnifera is a medicinal plant (Solanaceae) known for several biochemical properties attributed to its characteristic steroidal compounds in roots, stems, and leaves, called withanolides and glycowithanaloides (Mishra et al 2005;Lal et al 2006;Sharma et al 2007). Recent studies reveal that 3β-hydroxysterol glycosyltransferase in W. somnifera provide a wide range of biochemical properties.…”

Section: Bioconcentration Of Lindane In W Somniferamentioning

confidence: 99%

“…Recent studies reveal that 3β-hydroxysterol glycosyltransferase in W. somnifera provide a wide range of biochemical properties. It is proven that UDP-glycosyltransterases (UGTs) has a significant role in secondary metabolism (Sharma et al 2007). In higher plants, UGT-catalyzed glycosylation constitutes a prominent terminal modification in the biosynthesis of secondary metabolites and generates diverse natural glycosides (Bowles et al 2006).…”

Section: Bioconcentration Of Lindane In W Somniferamentioning

confidence: 99%

Withania somnifera Dunal-mediated dissipation of lindane from simulated soil: implications for rhizoremediation of contaminated soil

Abhilash

Singh

2009

J Soils Sediments

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Morphology and withanolide production of Withania coagulans hairy root cultures

Mirjalili¹,

Fakhr-Tabatabaei²,

Bonfill³

et al. 2009

Engineering in Life Sciences

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Inoculation of leaf sections of Withania coagulans with Agrobacterium tumefaciens strain C58C1 (pRiA4) induced transformed roots with the capacity to produce the most important bioactive compounds of Withania species, withanolide A and withaferin A. The hairy roots obtained showed two morphologies: callus‐like roots (CR) with a high capacity to produce withanolides and typical hairy roots (HR) with faster growth capacity and lower withanolide accumulation. The aux1 gene of pRiA4 was detected by PCR analyses in all roots showing callus‐like morphology. However, this gene was only detected in 12.5% of the roots showing typical hairy root morphology. This fact suggests a significant role of aux genes in the morphology of transformed roots. Time course studies of withanolide production showed that withanolide A accumulated during the first part of the culture whereas the maximum accumulation of withaferin A occurred at the end of the culture period. Some transformed root lines, such as HR112 and CR26, showed considerable potential to produce withanolides in a scaled up bioreactor system, especially the important pharmaceutical compound withanolide A.

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Molecular cloning and characterization of one member of 3β-hydroxy sterol glucosyltransferase gene family in Withania somnifera

Cited by 69 publications

References 37 publications

Metabolic shift from withasteroid formation to phenylpropanoid accumulation in cryptogein-cotransformed hairy roots of Withania somnifera (L.) Dunal

Metabolic shift from withasteroid formation to phenylpropanoid accumulation in cryptogein-cotransformed hairy roots of Withania somnifera (L.) Dunal

Withania somnifera Dunal-mediated dissipation of lindane from simulated soil: implications for rhizoremediation of contaminated soil

Morphology and withanolide production of Withania coagulans hairy root cultures

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