Molecular cloning and characterization of a heat shock protein 70 gene in swimming crab (Portunus trituberculatus)

Cui, Zhaoxia; Liu, Yuan; Luan, Weisha; Li, Qianqian; Wu, Dengsheng; Wang, Shuangyan

doi:10.1016/j.fsi.2009.09.018

Cited by 77 publications

(48 citation statements)

References 53 publications

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“…Induced expression of Hsp70 gene was noticed in sea bream liver tissue at 36 h post-infection with Vibrio alginolyticus (Deane et al 2004). The induction was not limited to fish, but higher expression was also reported in infected crabs, clams, and shrimps as well during Gram-negative bacterial exposure (Cui et al 2010;Rungrassamee et al 2010;Yue et al 2011). However, in the present study, Hsp70 gene was downregulated in liver and kidney tissues.…”

Section: Discussioncontrasting

confidence: 43%

Expression analysis of heat shock protein genes during Aeromonas hydrophila infection in rohu, Labeo rohita, with special reference to molecular characterization of Grp78

Das

Mohapatra

Sahoo

2015

Cell Stress and Chaperones

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Section: Discussioncontrasting

confidence: 43%

Expression analysis of heat shock protein genes during Aeromonas hydrophila infection in rohu, Labeo rohita, with special reference to molecular characterization of Grp78

Das

Mohapatra

Sahoo

2015

Cell Stress and Chaperones

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“…However, EsHSC70-3 expression was upregulated in all of the tissues at nearly all of the time points compared to the controls. High expression was also reported in infected crabs, clams, shrimps, and rohu, as well as during Gram-negative bacterial exposure (Cui et al, 2010;Rungrassamee et al, 2010;Das et al, 2015). M. nipponense HSC70 transcription initially increases and then decreases after challenge by A. hydrophila (Xiu et al, 2014), which is similar to the pattern of EsHSC70 transcription found in the present study.…”

Section: Discussionsupporting

confidence: 89%

“…Similar tissue-tropic expression of HSC70s has been reported in turbot Scophthalmus maximus (Wang et al, 2013) and oriental river prawns M. nipponense (Xiu et al, 2014). However, in the swimming crab Portunus trituberculatus, PtHSP70 expression gradually increases to a peak at 12 h in hemocytes after stimulation with V. alginolyticus (Cui et al, 2010), which is similar to that found with EsHSC70-5 in the hepatopancreas in the present study, but is very different to that found with EsHSC70-3. The EsHSC70-5 mRNA level was significantly downregulated in the intestine and gills at nearly all time points compared to the controls, and was expressed at a low level post-challenge.…”

Section: Discussionsupporting

confidence: 84%

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Expression patterns of two heat-shock cognate 70 genes during immune responses and larval development of the Chinese mitten crab Eriocheir sinensis

Jiang

Guo

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Two heat-shock protein (HSP) 70 family transcripts, heat-shock protein 70 cognate 5 and heat-shock protein 70 cognate 3 (designated as EsHSC70-5 and EsHSC70-3, respectively), were isolated from the Chinese mitten crab Eriocheir sinensis and their expression profiles were evaluated for their responsiveness to larval development and immune challenge in adult crabs. The HSPs exhibited 45-89% identity with other heat-shock proteins, and they shared similar structural features. EsHSC70 mRNA expression was detected not only during infection but also during the developmental larval stages. The EsHSC70s were enriched, and their expression fluctuated during early development. EsHSC70 mRNA expression was significantly induced by Vibrio parahaemolyticus challenge in all of the tissues studied (P < 0.05). Expression of EsHSC70 mRNA in the hepatopancreas and at the early zoeal stages was particularly pronounced, and the two EsHSC70s exhibited differential expression patterns both chronologically and spatially. The EsHSC70-5 mRNA level was significantly downregulated in the intestine and gills compared to that in controls at nearly all time points, and was expressed at a lower level after the bacterial challenge, indicating that EsHSC70-5 and EsHSC70-3 respond to immune challenges. The stage-specific enrichment of EsHSC70 transcripts in crabs suggests that these stress proteins play an essential role during brachyurization events.

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“…Although the saline adaptability of swimming crabs involves a series of reactions at the individual, protein, and molecular level, the osmotic regulation mechanism at the molecular level might underlie this species' adaption to saline conditions. The molecular characterization of HSP60, HSP70, and HSP90 from P. trituberculatus have been previously reported (Zhang et al, 2009;Cui et al, 2010;Xu and Qin, 2012). In this paper, the expression levels of HSP60, HSP70, HSP90-1, and HSP90-2 in the hepatopancreas of WG and SG swimming crabs, P. trituberculatus, during 72 h exposure to low salinity (4 ppt) were detected, with the purpose of identifying the mechanism of low salinity tolerance.…”

Section: Introductionmentioning

confidence: 87%

mRNA expression profiles of heat shock proteins of wild and salinity-tolerant swimming crabs, Portunus trituberculatus, subjected to low salinity stress

Bao

Zhang

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Challenged by the low salinity, 4 parts per thousand (4 ppt), for 72 h, the survivals of swimming crabs (Portunus trituberculatus) were collected as the screened group (SG, tolerant to low salinity). Aiming at identifying the mechanism of low salinity tolerance, quantitative real-time PCR was employed to investigate the expression profiles of 4 HSP genes (HSP60, HSP70, HSP90-1, HSP90-2) in the hepatopancreas of wild (WG) and screened (SG) groups of P. trituberculatus exposed to low salinity (4 ppt). The results showed that 3 of the candidate genes (HSP60, HSP70, HSP90-1) exhibited similarly downregulated expression profiles in the first 3 h (P < 0.05), which became upregulated from 3 h to 72 h after being subjected to low salinity conditions. In contrast, the expression profile of the HSP90-2 gene was upregulated during the first 6 h for the WG, and during the first 12 h for the SG, after which it became downregulated. HSP90-1 and HSP90-2 were highly expressed at 12 h after low salinity challenge in the SG, but not the WG. The response of these 2 genes to salinity stress indicates their suitability as biomarkers to differentiate SG from WG crabs. The results indicate that HSP genes are involved in the adaptation of crabs to low salinity exposure, and that different HSPs have diverse functions in response to low salinity stress in P. trituberculatus. In addition, HSP expression in SG indicates that this group is more tolerant to low salinity conditions compared to WG.

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Molecular cloning and characterization of a heat shock protein 70 gene in swimming crab (Portunus trituberculatus)

Cited by 77 publications

References 53 publications

Expression analysis of heat shock protein genes during Aeromonas hydrophila infection in rohu, Labeo rohita, with special reference to molecular characterization of Grp78

Expression analysis of heat shock protein genes during Aeromonas hydrophila infection in rohu, Labeo rohita, with special reference to molecular characterization of Grp78

Expression patterns of two heat-shock cognate 70 genes during immune responses and larval development of the Chinese mitten crab Eriocheir sinensis

mRNA expression profiles of heat shock proteins of wild and salinity-tolerant swimming crabs, Portunus trituberculatus, subjected to low salinity stress

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