Molecular cloning and characterization of C1 tetrahydrofolate (C1-THF) synthase in Bombyx mori, silkworm

Chen, Meijun; Zhai, Jingjie; Yu, Li; Xue, Bin; Hu, Jiahuan; Cheng, Xiaoyu; Li, Jinxin; Hu, Jingsheng

doi:10.1016/j.gene.2018.04.037

Cited by 7 publications

(6 citation statements)

References 23 publications

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“…The fat body is an important organ with the main function to synthesize, store, and transport nutrients and plays a critical role in protein metabolism and detoxification . Amino acid and nitrogen metabolism, sugar and lipid metabolism, and so forth are examples of some of the majority of insect intermediate metabolic responses that occur in fat bodies.…”

Section: Results and Discussionmentioning

confidence: 99%

“…The fat body is an important organ with the main function to synthesize, store, and transport nutrients and plays a critical role in protein metabolism and detoxification. 51 Amino acid and nitrogen metabolism, sugar and lipid metabolism, and so forth are examples of some of the majority of insect intermediate metabolic responses that occur in fat bodies. In order to perform a wide range of metabolic functions, fat bodies can synchronize with other organs, promote the process of nutrition metabolism within the body, and adapt to the everchanging physiological requirements of insects throughout growth and development.…”

Section: Verification Of Differentially Expressed Genes With Qrt-pcrmentioning

confidence: 99%

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Cytotoxicity of AuCu–Cu₂S Nanocomposites: Implications for Biological Evaluation of the Nanocomposite Effect on Bombyx mori Silkworms and Cell Lines

Andoh,

Shi,

et al. 2023

ACS Biomater. Sci. Eng.

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AuCu–Cu2S nanocomposites are unique materials with exceptional properties that have recently received a lot of interest. However, little is known about their potential toxicity in terrestrial organisms and their subsequent effects on the environment. Therefore, it is essential to develop effective methodologies for evaluating AuCu–Cu2S nanocomposites in biological systems. This study reports the biological evaluation of the AuCu–Cu2S nanocomposite from animal and cell entity levels. The Bombyx mori silkworm was used as a model organism to study the effects of different concentrations of AuCu–Cu2S on silkworm development. Transcriptome analysis was also carried out to examine the genetic modulation exerted by the treatment. Moreover, biocompatibility and cytotoxicity of AuCu–Cu2S were evaluated in human bronchial epithelial cells 16HBE, human lung adenocarcinoma, and the insect Spodoptera frugiperda cell sf9 cell lines. The results showed that although AuCu–Cu2S at ≤400 ppm can prolong the eating habit of silkworms and promote the weight of the cocoon layer, there was an increase in silkworm mortality and a decrease in moth formation at a concentration of ≥800 ppm. The genetic regulation by AuCu–Cu2S treatment showed varying effects in the silkworm, primarily related to functions such as transport and catabolism, metabolism of cofactors and vitamins, xenobiotic biodegradation, amino acid, and carbohydrate. 16HBE, PC-9, and sf9 treated with 300 ppm of AuCu–Cu2S showed viability percentages of 60, 20, and 90%, respectively. Thus, AuCu–Cu2S at low concentrations serves as a safe and biocompatible material for the sf9 cell lines but is lethal to 16HBE and PC-9. This research could aid in understanding the biological effects and biocompatibility of AuCu–Cu2S nanocomposites, particularly in the field of biochemistry; however, the mechanisms involved need further exploration.

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Section: Results and Discussionmentioning

confidence: 99%

Section: Verification Of Differentially Expressed Genes With Qrt-pcrmentioning

confidence: 99%

Cytotoxicity of AuCu–Cu₂S Nanocomposites: Implications for Biological Evaluation of the Nanocomposite Effect on Bombyx mori Silkworms and Cell Lines

Andoh,

Shi,

et al. 2023

ACS Biomater. Sci. Eng.

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“…Chen et al . (2018) showed that a silkworm's fat body produces more C1‐THF synthase and demonstrated its involvement in oxidative stress regulation. However, its function in insect reproduction is still not clear.…”

Section: Resultsmentioning

confidence: 99%

A comparative protein profile of accessory glands of virgin and matedLeucinodes orbonalismales

et al. 2020

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Male accessory gland (MAG) secretory proteins affect female reproductive physiology and behaviour when they are delivered to the female at the time of mating along with sperm. In our study, proteomic approaches were used to identify MAG proteins of Leucinodes orbonalis, a monophagous and destructive pest of brinjal. A set of 117 and 186 MAG spots were observed in virgin and mated males, respectively, using two‐dimensional gel electrophoresis (2D‐GE). Among the identified spots, 49 are unique to the virgin, whereas 118 are unique to mated MAGs, with 68 spots found to be common. The differentially expressed MAG proteins, 14 are up‐regulated, and 16 are down‐regulated in virgin after mating. We used matrix‐assisted laser desorption ionization (MALDI)–mass spectrometry (MS) to identify the 13 unique proteins in the MAG of virgin L. orbonalis and analysed the data of the Swiss‐Prot database using the Mascot search engine. The proteins were identified as proteolysis regulators, lipid transporters, olfactory proteins, metabolism‐related proteins, DNA‐binding proteins, and hexamerins. This is the first report on the gel‐based protein analysis of L. orbonalis MAGs.

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“…Studies have also shown that the expression of many genes undergoes regulatory changes in the epidermis during molting stages (Yang et al, 2016; J. Zhang, Lu, Kong, Zhang, & Ling, 2014). The gene BmC 1 ‐THF encodes a crucial enzyme in folate metabolism and is important for the synthesis of nucleic acids and proteins as well as DNA methylation and repair (Chen et al, 2018). Expression of BmC 1 ‐THF is elevated in the a42 mutant and may influence the phenotype observed at the first instar larval stage of a42 .…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis at the first instar larval stage of nonmolting Bombyx mori mutant (a42)

Sun

Zhang

Qin

et al. 2020

Arch Insect Biochem Physiol

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The life cycle of the holometabolous insect Bombyx mori (Linnaeus) consists of the embryo, larva, pupa, and adult stages with six larval molts. Ecdysone and juvenile hormones play important roles in the growth and development of the silkworms. The a42 silkworm mutant is recessive and homozygous lethal by exhibiting a dark‐colored and small body size and fails to molt to second instar. We compared the gene expression of a42 mutants with normal individuals at the first larval molting stage to elucidate the physiological influence of the a42 mutation on the growth and development of silkworms. The transcriptomic sequencing results revealed that 1,411 genes are differentially expressed in a42 mutants, compared with wild‐type control silkworms, in which 791 genes are upregulated and 620 genes are downregulated. Gene Ontology/Kyoto Encyclopedia of Genes and Genomes analyses identified differentially expressed genes (DEGs) assigned to biological pathways, such as pentose and glucoronate interconversions, glycerolipid metabolism, folate biosynthesis, amino sugar, and nucleotide sugar metabolism. Two hydroxylases of phenylalanine hydroxylase (BmPAH) and tyrosine hydroxylase (BmTh) are upregulated in a42 mutants. The influence of a42 mutation on these DEGs reveals that melanin metabolism plays an important role during the molting process in silkworms.

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Molecular cloning and characterization of C1 tetrahydrofolate (C1-THF) synthase in Bombyx mori, silkworm

Cited by 7 publications

References 23 publications

Cytotoxicity of AuCu–Cu₂S Nanocomposites: Implications for Biological Evaluation of the Nanocomposite Effect on Bombyx mori Silkworms and Cell Lines

Cytotoxicity of AuCu–Cu₂S Nanocomposites: Implications for Biological Evaluation of the Nanocomposite Effect on Bombyx mori Silkworms and Cell Lines

A comparative protein profile of accessory glands of virgin and matedLeucinodes orbonalismales

Transcriptomic analysis at the first instar larval stage of nonmolting Bombyx mori mutant (a42)

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Molecular cloning and characterization of C1 tetrahydrofolate (C1-THF) synthase in Bombyx mori, silkworm

Cited by 7 publications

References 23 publications

Cytotoxicity of AuCu–Cu2S Nanocomposites: Implications for Biological Evaluation of the Nanocomposite Effect on Bombyx mori Silkworms and Cell Lines

Cytotoxicity of AuCu–Cu2S Nanocomposites: Implications for Biological Evaluation of the Nanocomposite Effect on Bombyx mori Silkworms and Cell Lines

A comparative protein profile of accessory glands of virgin and matedLeucinodes orbonalismales

Transcriptomic analysis at the first instar larval stage of nonmolting Bombyx mori mutant (a42)

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Product

Resources

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Cytotoxicity of AuCu–Cu₂S Nanocomposites: Implications for Biological Evaluation of the Nanocomposite Effect on Bombyx mori Silkworms and Cell Lines

Cytotoxicity of AuCu–Cu₂S Nanocomposites: Implications for Biological Evaluation of the Nanocomposite Effect on Bombyx mori Silkworms and Cell Lines