Molecular cloning and characterization of the SIFamide precursor and receptor in a hymenopteran insect, Bombus terrestris

Lismont, Els; Mortelmans, Nele; Verlinden, Heleen; Broeck, Jozef Vanden

doi:10.1016/j.ygcen.2017.10.014

Cited by 10 publications

(8 citation statements)

References 97 publications

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“…The aim of this study was to investigate the possible role of Rhopr-SIFa, a member of a highly conserved family, in feeding in R. prolixus. We also show the presence of SLI in processes in the CC and along the dorsal vessel, suggesting peripheral actions of Rhopr-SIFa and possibly a neurohormonal function; providing a rationale for the existence of the SIFa receptor (SIFaR) in peripheral tissues as discussed in Lismont et al (2018).…”

Section: Discussionmentioning

confidence: 53%

“…These findings support at the least a peripheral role for Rhopr-SIFa, but also is suggestive of a possible neurohormonal role whereby Rhopr-SIFa may be released from the CC and dorsal vessel, and act peripherally on the posterior dorsal vessel, which controls heartbeat frequency. Since the SIFa receptor has been shown to be expressed in peripheral tissues in B. terrestris (Lismont et al, 2018) it will be critical to determine if the same is true for the receptor in R. prolixus, since a Rhopr-SIFa receptor has been predicted in the R. prolixus genomic sequence (Ons et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…The role of SIFa as a neurohormone has been debated with initial reports maintaining it was only found within the central nervous system (CNS) of Drosophila melanogaster (Terhzaz et al, 2007;Gellerer et al, 2015). Lismont et al (2018), however, found the SIFamide receptor transcript to be expressed in the periphery of Bombus terrestris suggesting that SIFamide may circulate in the hemolymph and control peripheral tissues. Although the physiological effects of SIFamide on peripheral tissues has not been explored, multiple functions for SIFamide have been reported in a variety of insects.…”

Section: Introductionmentioning

confidence: 99%

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SIFamide Influences Feeding in the Chagas Disease Vector, Rhodnius prolixus

et al. 2020

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SIFamides are a family of highly conserved neuropeptides in arthropods, and in insects are mainly expressed in four medial neurons in the pars intercerebralis of the brain. Although SIFamide has been shown to influence sexual behavior, feeding, and sleep regulation in holometabolous insects such as Drosophila melanogaster, little is known about its role in hemimetabolous insects, including the blood-sucking bug, Rhodnius prolixus. In this study, we confirm the nucleotide sequence for R. prolixus SIFamide (Rhopr-SIFa) and find characteristic phenotypic expression of SIFamide in four cells of the pars intercerebralis in the brain. In addition to extensive SIFa projections throughout the entire central nervous system, SIFamidergic processes also enter into the corpus cardiacum, and project along the dorsal vessel, suggestive of Rhopr-SIFa acting as a neurohormone. Physiologically, Rhopr-SIFamide induces dose-dependent increases in heartbeat frequency in vitro suggesting the presence of peripheral receptors, and thereby indicating Rhopr-SIFa is released to act upon peripheral targets. We also explore the function of Rhopr-SIFa in R. prolixus, specifically in relation to feeding, since R. prolixus is a blood-gorging insect and a vector for Chagas disease. The intensity of SIFamide-like staining in the neurons in the brain is diminished 2 h following feeding, and restocking of those cells is finished 24 h later, indicating Rhopr-SIFa may be released at feeding. The results of temporal qPCR analysis were consistent with the immunohistochemical findings, showing an increase in Rhopr-SIFa transcript expression in the brain 2 h after feeding. We also observed enhanced feeding (size of meal) in insects injected with Rhopr-SIFa whereas insects with RNAimediated knockdown of the Rhopr-SIFa transcript consumed a significantly smaller blood meal relative to controls. These data suggest that the four SIFamidergic neurons and associated arborizations may play an important function in the neuronal circuitry controlling R. prolixus feeding, with Rhopr-SIFa acting as a central and peripheral neuromodulator/neurohormone.

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Section: Discussionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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SIFamide Influences Feeding in the Chagas Disease Vector, Rhodnius prolixus

et al. 2020

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“…The production and functions of peptide hormones are becoming increasingly clear, especially with respect to the mechanisms of action of steroids and several neuropeptides. In addition to the functions discussed above, insects have certain peptideregulated behaviors; for example, insect SIFamide is a neuropeptide produced by four medial interneurons, which can affect sexual behavior, sleep, death, and pupa number (Lismont et al, 2018). Insect diapause hormone is a type of peptide that can help individuals survive in a changing environment, such as overwintering; some insects exhibit embryonic diapause, while others exhibit pupal diapause (Zhang et al, 2015b).…”

Section: Resultsmentioning

confidence: 99%

Peptide Hormones in the Insect Midgut

Liu

Wang

et al. 2020

Front. Physiol.

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Insects produce many peptide hormones that play important roles in regulating growth, development, immunity, homeostasis, stress, and other processes to maintain normal life. As part of the digestive system, the insect midgut is also affected by hormones secreted from the prothoracic gland, corpus allatum, and various neuronal cells; these hormones regulate the secretion and activity of insects' digestive enzymes and change their feeding behaviors. In addition, the insect midgut produces certain hormones when it recognizes various components or pathogenic bacteria in ingested foods; concurrently, the hormones regulate other tissues and organs. In addition, intestinal symbiotic bacteria can produce hormones that influence insect signaling pathways to promote host growth and development; this interaction is the result of long-term evolution. In this review, the types, functions, and mechanisms of hormones working on the insect midgut, as well as hormones produced therein, are reviewed for future reference in biological pest control.

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“…5). One branch contains the deorphanized Drosophila and Bombus terrestris SIFamide receptors (Jørgensen et al, 2006; Lismont et al, 2018), the other the deorphanized Ixodes -2 SIFamide receptor (Šimo et al, 2013). Furthermore, in the planthopper Nilaparvata lugens no SMYamide gene can be found, but it does have two SIFamide receptors (Tanaka et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

The neuropeptide SMYamide, a SIFamide paralog, is expressed by salivary gland innervating neurons in the American cockroach and likely functions as a hormone

Veenstra

2020

Preprint

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The SMYamide genes are paralogs of the SIFamide genes and code for neuropeptides that are structurally similar to SIFamide. In the American cockroach, Periplanea americana, the SMYamide gene is specifically expressed in the SN2 neurons that innervate the salivary glands and are known to produce action potentials during feeding. The innervation of the salivary glands by the SN2 neurons is such that one has to expect that on activation of these neurons significant amounts of SMYamide will be released into the hemolymph, thus suggesting that SMYamide also functions as a hormone. In the Periplaneta genome there are two putative SIFamide receptors and these are both expressed not only in the central nervous system and the salivary gland, but also in the gonads and other peripheral tissues. This reinforces the hypothesis that SMYamide also has an endocrine function in this species.

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Molecular cloning and characterization of the SIFamide precursor and receptor in a hymenopteran insect, Bombus terrestris

Cited by 10 publications

References 97 publications

SIFamide Influences Feeding in the Chagas Disease Vector, Rhodnius prolixus

SIFamide Influences Feeding in the Chagas Disease Vector, Rhodnius prolixus

Peptide Hormones in the Insect Midgut

The neuropeptide SMYamide, a SIFamide paralog, is expressed by salivary gland innervating neurons in the American cockroach and likely functions as a hormone

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