Molecular cloning and characterization of a phospholipid hydroperoxide glutathione peroxidase gene from a blood fluke Schistosoma japonicum

Zhang, Ying; He, Yu; He, Li; Zong, Hong; Cai, Guobin

doi:10.1016/j.molbiopara.2015.10.001

Cited by 6 publications

(1 citation statement)

References 46 publications

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“…These enzymes are engaged in decomposing lipid hydroperoxides. It was reported that protein levels and enzymatic activity of phospholipid hydroperoxide glutathione peroxidase from Schistosoma japonicum can be stimulated by incubation of adult worms with millimolar amounts of oxidative molecules, such as paraquat or hydrogen peroxide25.…”

Section: Resultsmentioning

confidence: 99%

RNA-seq analyses of the midgut from blood- and serum-fed Ixodes ricinus ticks

Perner

Provazník

Schrenková

et al. 2016

Sci Rep

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Adult females of the genus Ixodes imbibe blood meals exceeding about 100 times their own weight within 7‒9 days. During this period, ticks internalise components of host blood by endocytic digest cells that line the tick midgut epithelium. Using RNA-seq, we aimed to characterise the midgut transcriptome composition in adult Ixodes ricinus females during early and late phase of engorgement. To address specific adaptations to the haemoglobin-rich diet, we compared the midgut transcriptomes of genetically homogenous female siblings fed either bovine blood or haemoglobin-depleted serum. We noted that tick gut transcriptomes are subject to substantial temporal-dependent expression changes between day 3 and day 8 of feeding. In contrast, the number of transcripts significantly affected by the presence or absence of host red blood cells was low. Transcripts relevant to the processes associated with blood-meal digestion were analysed and involvement of selected encoded proteins in the tick midgut physiology discussed. A total of 7215 novel sequences from I. ricinus were deposited in public databases as an additional outcome of this study. Our results broaden the current knowledge of tick digestive system and may lead to the discovery of potential molecular targets for efficient tick control.

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Section: Resultsmentioning

confidence: 99%

RNA-seq analyses of the midgut from blood- and serum-fed Ixodes ricinus ticks

Perner

Provazník

Schrenková

et al. 2016

Sci Rep

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Expression and characterization of glutathione peroxidase of the liver fluke, Fasciola gigantica

et al. 2018

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Glutathione peroxidase (GPx) is a key member of the family of antioxidant enzymes in trematode parasites including Fasciola spp. Because of its abundance and central role as an anti-oxidant that helps to protect parasites from damage by free radicals released from the host immune cells, it has both diagnostic as well as vaccine potential against fasciolosis. In this study, we have cloned, characterized, and detected the expression of the GPx protein in Fasciola gigantica (Fg). FgGPx (582 bp) was cloned by polymerase chain reaction (PCR) from complementary DNA (cDNA) from an adult fluke. Its putative peptide has no signal sequence and is composed of 168 amino acids, with a molecular weight of 19.1 kDa, and conserved sequences at NVACKUG, FPCNQFGGQ, and WNF. Phylogenetic analysis showed that GPx is present from protozoa to mammals and FgGPx was closely related to Fasciola hepatica GPx. A recombinant FgGPx (rFgGPx) was expressed in Escherichia coli BL21 (DE3) and used for immunizing mice to obtain polyclonal antibodies (anti-rFgGPx) for immunoblotting and immunolocalization. In immunoblotting analysis, the FgGPx was expressed in all stages of F. gigantica (eggs, metacercariae, newly excysted juveniles (NEJ), 4-week-old juveniles, and adults). This mouse anti-rFgGPx reacted with the native FgGPx at a molecular weight of 19.1 kDa in adult whole body (WB) and tegumental antigens (TA) as detected by immunoblotting. The FgGPx protein was expressed at a high level in the tegument, vitelline glands, and eggs of the parasite. Anti-rFgGPx exhibited no cross-reactivity with the other parasite antigens, including Eurytrema pancreaticum, Cotylophoron cotylophorum, Fischoederius cobboldi, Gastrothylax crumenifer, Paramphistomum cervi, and Setaria labiato papillosa. The possibility of using rFgGPx for immunodiagnosis and/or as a vaccine for fasciolosis in animals of economic importance will be explored in the future.

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Identification of two putative phospholipid hydroperoxide glutathione peroxidase genes and the induction of three environmental stresses in Neoseiulus barkeri (Acari: Phytoseiidae)

Tian

Zhang

et al. 2017

Journal of Asia-Pacific Entomology

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Molecular cloning and characterization of a phospholipid hydroperoxide glutathione peroxidase gene from a blood fluke Schistosoma japonicum

Cited by 6 publications

References 46 publications

RNA-seq analyses of the midgut from blood- and serum-fed Ixodes ricinus ticks

RNA-seq analyses of the midgut from blood- and serum-fed Ixodes ricinus ticks

Expression and characterization of glutathione peroxidase of the liver fluke, Fasciola gigantica

Identification of two putative phospholipid hydroperoxide glutathione peroxidase genes and the induction of three environmental stresses in Neoseiulus barkeri (Acari: Phytoseiidae)

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