Molecular Cloning and Characterization of a Novel Testis-Specific Nucleoporin-Related Gene

Lf, Wang; Hd, Zhu; Sy, Miao; Df, Cao; Wu, Yen‐Wen; Sd, Zong; Ss, Koide

doi:10.1080/014850199262904

Cited by 17 publications

(16 citation statements)

References 36 publications

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“…This experiment was repeated three times with comparable results. acrosome to cluster at the caudal end of the nucleus [16][17][18][19][20]. We next wanted to determine whether the distribution of NUP62 differs from that of the other nucleoporins, as was suggested by the sucrose gradient results mentioned previously (Fig.…”

Section: Nup62 Redistributes In Male Germ Cells Differently Than Othementioning

confidence: 97%

The Molecular Motor KIFC1 Associates with a Complex Containing Nucleoporin NUP62 That Is Regulated During Development and by the Small GTPase RAN1

Yang

Jefferson

Sperry

2006

Biology of Reproduction

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KIFC1 is a C-terminal kinesin motor associated with the nuclear membrane and acrosome in round and elongating spermatids. This location in developing spermatids is consistent with possible roles in acrosome elongation and manchette motility or both. Here we describe the association of the KIFC1 motor with a complex containing the nucleoporin NUP62. Formation of this complex is developmentally regulated, being absent before puberty and appearing only after nuclear elongation has begun. In addition, the integrity of this complex is dependent on GTP hydrolysis and the GTP state of the small GTPase RAN. Concomitant with the association of this motor with the NUP62-containing complex is an apparent reorganization of the nuclear pore with loss of NUP62 from larger complexes containing other nucleoporins. The association of KIFC1 with a component of the nuclear membrane is more consistent with a role for this motor in acrosome/manchette transport along the nuclear membrane than for a role for this motor in transport of vesicles along the outer face of the manchette.

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Section: Nup62 Redistributes In Male Germ Cells Differently Than Othementioning

confidence: 97%

The Molecular Motor KIFC1 Associates with a Complex Containing Nucleoporin NUP62 That Is Regulated During Development and by the Small GTPase RAN1

Yang

Jefferson

Sperry

2006

Biology of Reproduction

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“…The NPC outer diameter is about 120 nm having an estimated Mr of 125 kDa. It is interesting to note that a component of human sperm tails has been identified as a nucleoporin‐related protein (110). Moreover, nucleoporin‐related mRNA has been shown to be transcribed in germ cells of rat testis (111).…”

Section: Testis Nucleoporin‐related Componentmentioning

confidence: 99%

“…DIG‐labeled RSD‐1 (2069 base pairs) was prepared and used as a probe to screen a human testis λZAPII cDNA library. A positive clone was isolated that yielded a cDNA consisting of 1933 base pairs, with an open reading frame composed of 1824 base pairs, encoding a polypeptide, containing 608 amino acid residues (110). The cDNA and deduced polypeptide were designated as BS‐63 and assigned the accession number U64675 by GenBank.…”

Section: Testis Nucleoporin‐related Componentmentioning

confidence: 99%

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Antisperm Antibodies Associated with Infertility: Properties and Encoding Genes of Target Antigens

Koide

Wang

Kamada

2000

Proc Soc Exp Biol Med

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Abstract. Infertility among couples of reproductive age is a perplexing condition when the cause is indeterminate. These cases are classified as unexplained infertility. In a subset of subjects, antisperm antibodies with sperm agglutinating and/or immobilizing activities have been detected in the blood or fluids of the reproductive tract. These cases are designated as immunologic infertility although a cause and effect relationship of the antibodies to infertility has not been established. In this review, seven target sperm antigens to antibodies associated with infertility and their encoding genes are described. The antisperm antibodies (ASAs) examined were obtained from infertile women or were monoclonal antibodies (mAb) raised against human sperm proteins. All the ASAs studied possessed potent sperm agglutinating and/or immobilizing activities. The target antigens were isolated from human and other mammalian sperm, and the encoding genes identified. The seven antigens are YWK‐II, BE‐20, rSMP‐B, BS‐63 (nucleoporin‐related), BS‐17 (calpastatin), HED‐2 (zyxin), and 75‐ kDa. Each antigen is a distinct and separate entity and is produced by different cells of the reproductive tract, (e.g., germ cells, epididymal epithelial cells, and Sertoli cells). No single predominant target component has been found to interact with the ASAs. It is proposed that immunologic infertility is the consequence of the combined actions of multiple ASAs in immobilizing and/or agglutinating spermatozoa, blocking spermegg interaction, preventing implantation, and/or arresting embryo development.

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“…Researchers at the Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences have cloned a large number of spermatogenesis-related genes; for example, SPATA7 that encodes a meiotic-related protein expressed in rat and human testis [116]; ZYX that encodes a protein that is linked to sperm maturation and capacitation [117]; VCX/Y that encodes a protein that might participate in regulating the assembly of ribosomes during spermatogenesis [118]; RGPD5 whose mRNA is expressed only in germ cells at all stages of spermatogenesis and is closely associated with spermatogenesis [119]; and BS-63 that encodes a protein that acts as a carrier system in transporting aF10 into the nucleus of germ cells during spermiogenesis [120]. Genes related to the genesis of esophageal cancer have also been cloned at the institute.…”

Section: Svh Has Four Splicing Variants Svh-a Svh-b Svh-c and Svh-d;mentioning

confidence: 99%

Literature and patent analysis of the cloning and identification of human functional genes in China

Xia

Tang

Yao

et al. 2012

Sci. China Life Sci.

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Molecular Cloning and Characterization of a Novel Testis-Specific Nucleoporin-Related Gene

Cited by 17 publications

References 36 publications

The Molecular Motor KIFC1 Associates with a Complex Containing Nucleoporin NUP62 That Is Regulated During Development and by the Small GTPase RAN1

The Molecular Motor KIFC1 Associates with a Complex Containing Nucleoporin NUP62 That Is Regulated During Development and by the Small GTPase RAN1

Antisperm Antibodies Associated with Infertility: Properties and Encoding Genes of Target Antigens

Literature and patent analysis of the cloning and identification of human functional genes in China

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