Molecular cloning and characterization of two genes encoding peroxiredoxins from freshwater bivalve Anodonta woodiana: Antioxidative effect and immune defense

Xia, Xuan; Yu, Rong; Li, Mengbo; Liu, Li; Zhang, Ke; Wang, Yu; Li, Bingjie; Zhang, Linguo; Song, Guoying; Zheng, Xinhua; Bai, Xia

doi:10.1016/j.fsi.2018.08.050

Cited by 14 publications

(7 citation statements)

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“…Mussels with spermatozoa were considered as male. According to previous investigations, 30,32 240 male clams A. woodiana were randomly grouped into BPA-treated groups in which animals were treated with five test concentrations of BPA, and a control group with similar volume of DMSO. Concentration ranges for viability studies were 125 ng/l, 250 ng/l, 500 ng/l, 1000 ng/l and 2000 ng/l for BPA.…”

Section: Methodsmentioning

confidence: 99%

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Molecular characterization of AwSox2 from bivalve Anodonta woodiana: Elucidating its player in the immune response

et al. 2019

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Sox2 is an embryonal stem cell Ag essential for early embryonic development, tissue homeostasis and immune regulation. In the current study, one complete Sox2 cDNA sequence was cloned from freshwater bivalve Anodonta woodiana and named AwSox2. Histological changes of testis derived from Bisphenol A (BPA) treatment were analyzed by hematoxylin and eosin staining. Expressions of AwSox2 derived from BPA, LPS and polyinosinic:polycytidylic (Poly I:C) challenge were measured by quantitative real-time PCR. The full-length cDNA of AwSox2 contained an open reading frame of 927 nucleotides bearing the typical structural features of Sox2 family. Obvious degeneration, irregular arrangement of spermatids, and clotted dead and intertwined spermatids were observed in BPA-treated groups. Administration of BPA could result in a dose-dependent up-regulation of AwSox2 expression in the male gonadal tissue of A. woodiana. In addition, expression of AwSox2 was significantly induced by LPS and Poly I:C treatment in the hepatopancreas, gill and hemocytes, compared with that of control group. These results indicated that up-regulations of AwSOx2 are closely related to apoptosis of spermatogonial stem cells derived from BPA treatment as well as enhancement of immune defense against LPS and Poly I:C challenge in A. woodiana.

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Section: Methodsmentioning

confidence: 99%

“…To determine the immune responses of AwSox2, LPS and Poly I:C (Sigma-Aldrich, St. Louis, MO) were selected as immune-stimulants in time-course experiments in accordance with previous studies. 32 LPS and Poly I:C were suspended in PBS. According to the above-mentioned culture conditions, 60 clams were treated with PBS in the control group.…”

Section: Methodsmentioning

confidence: 99%

Molecular characterization of AwSox2 from bivalve Anodonta woodiana: Elucidating its player in the immune response

et al. 2019

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“…EIF2 kinases include at least four genes (EIF2AK1-4) involved in the down-regulation of protein synthesis in response to various forms of cellular stress (Hinnebusch 1994), with EIF2AK3 (or PERK) being one of the three molecular sensors that control the activation of the unfolded protein response (Korennykh and Walter 2012). PRDX or Prx form a large family of antioxidant enzymes playing a fundamental role in protecting cells from oxidative damage (Wood et al 2003), and suggestively, have been found to be upregulated in response to stress in the snail P. canaliculata as well as in the unionid bivalve A. woodiana, both inhabiting freshwater environments (Liu et al 2018;Xia et al 2018).…”

Section: Parallel Gene Repertoire Expansions During Transitions Out O...mentioning

confidence: 99%

Unveiling the gene repertoire evolutionary dynamics driving repeated transitions out of the sea across molluscs

Aristide,

Fernández

2023

Preprint

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Animals abandoned their marine niche and successfully adapted to life on land multiple times throughout evolution, providing a rare opportunity to study the mechanisms driving large scale macroevolutionary convergence. However, the genomic factors underlying this process remain largely unknown. Here, we investigate the macroevolutionary dynamics of gene repertoire evolution during repeated transitions out of the sea in molluscs, a lineage that has transitioned to freshwater and terrestrial environments multiple independent times. Through phylogenomics and phylogenetic comparative methods, we examine approximately 100 genomic datasets encompassing all major molluscan lineages. We introduce a conceptual framework for identifying and analyzing parallel and convergent evolution at the orthogroup level (groups of genes derived from a single ancestral gene in the species in question), and explore the extent of these mechanisms. Despite deep temporal divergences, we found that expansions of ancient gene families played a major role in facilitating adaptation to non-marine habitats, highlighting the relevance of the pre-existing genomic toolkit in facilitating adaptation to new environments. The expanded functions primarily involve metabolic, osmoregulatory, and defense-related systems. We further found evidence of functionally convergent lineage-exclusive gene gains, while family contractions appear to be driven by neutral processes. Also, genomic innovations likely contributed to fuel independent habitat transitions. Overall, our study reveals that various mechanisms of gene repertoire evolution -parallelism, convergence and innovation- can simultaneously contribute to major evolutionary transitions. Our results provide a genome-wide gene repertoire atlas of molluscan terrestrialization that pave the way towards further understanding the functional and evolutionary bases of this process.

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“…The measure of intracellular Prdx levels can be used in the biomonitoring of the aquatic environment as a critical indicator of pollution [ 28 ]. However, only a few Prdxs have been studied in aquatic invertebrates, mostly in crustaceans and mollusks [ 29 , 30 ], and the modulation of their synthesis by various pollutants needs to be explored. Therefore, this study aims to increase our knowledge of ROS scavenging in invertebrates exposed to high metal ion concentrations, using Ciona robusta as a reference organism.…”

Section: Introductionmentioning

confidence: 99%

Typical 2-Cys Peroxiredoxins as a Defense Mechanism against Metal-Induced Oxidative Stress in the Solitary Ascidian Ciona robusta

et al. 2021

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Typical 2-Cys peroxiredoxins (2-Cys Prdxs) are proteins with antioxidant properties belonging to the thioredoxin peroxidase family. With their peroxidase activity, they contribute to the homeostatic control of reactive oxygen species (ROS) and, therefore, participate in various physiological functions, such as cell proliferation, differentiation, and apoptosis. Although Prdxs have been shown to be potential biomarkers for monitoring aquatic environments, minimal scientific attention has been devoted to describing their molecular architecture and function in marine invertebrates. Our study aims to clarify the protective role against stress induced by exposure to metals (Cu, Zn, and Cd) of three Prdxs (Prdx2, Prdx3, and Prdx4) in the solitary ascidian Ciona robusta, an invertebrate chordate. Here, we report a detailed pre- and post-translational regulation of the three Prdx isoforms. Data on intestinal mRNA expression, provided by qRT-PCR analyses, show a generalized increase for Prdx2, -3, and -4, which is correlated to metal accumulation. Furthermore, the increase in tissue enzyme activity observed after Zn exposure is slower than that observed with Cu and Cd. The obtained results increase our knowledge of the evolution of anti-stress proteins in invertebrates and emphasize the importance of the synthesis of Prdxs as an efficient way to face adverse environmental conditions.

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Molecular cloning and characterization of two genes encoding peroxiredoxins from freshwater bivalve Anodonta woodiana: Antioxidative effect and immune defense

Cited by 14 publications

References 62 publications

Molecular characterization of AwSox2 from bivalve Anodonta woodiana: Elucidating its player in the immune response

Molecular characterization of AwSox2 from bivalve Anodonta woodiana: Elucidating its player in the immune response

Unveiling the gene repertoire evolutionary dynamics driving repeated transitions out of the sea across molluscs

Typical 2-Cys Peroxiredoxins as a Defense Mechanism against Metal-Induced Oxidative Stress in the Solitary Ascidian Ciona robusta

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