Molecular cloning and expression analysis of α2-macroglobulin in the kuruma shrimp, Marsupenaeus japonicus

Rattanachai, Achara; Hirono, Ikuo; Ohira, Tsuyoshi; Takahashi, Yuichiro; Aoki, Takashi

doi:10.1016/j.fsi.2003.09.011

Cited by 68 publications

(51 citation statements)

References 41 publications

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“…A2M expression has been more thoroughly studied in mammalian ovaries, where it regulates protease activity necessary for ovulation and associated tissue remodeling [53,54]. Other studies of A2M expression in crustacean tissues have found that A2M transcripts are expressed most strongly in hemocytes [52,55,56] and that expression can be induced by microbial challenge [52,55,57]. Hemocytes were not analyzed in our study, and we anticipate that we might find elevated expression of A2M in hemocytes of symptomatic lobsters.…”

Section: Discussionmentioning

confidence: 77%

“…Although the difference was not significant, the statistical power was relatively low (due to the small sample size for ovarian tissue). Most studies have not explicitly quantified A2M expression in crustacean ovary, although one study reports weak expression in ovary of kuruma shrimp [52]. A2M expression has been more thoroughly studied in mammalian ovaries, where it regulates protease activity necessary for ovulation and associated tissue remodeling [53,54].…”

Section: Discussionmentioning

confidence: 99%

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Altered gene expression associated with epizootic shell disease in the American lobster, Homarus americanus

Tarrant

Stegeman

Verslycke

2010

Fish & Shellfish Immunology

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Epizootic shell disease is a poorly understood condition that has significantly affected the American lobster fishery in New England (northeastern US) since the 1990s. Here we present the results of a study to identify changes in gene expression in lobsters exhibiting symptoms of epizootic shell disease. Suppressive subtractive hybridization (SSH) was used to compare gene expression between cDNA pools from diseased (symptomatic) and apparently healthy (asymptomatic) lobsters. Subsequently, quantitative real-time polymerase chain reaction (qPCR) was used to measure expression of nine genes that were differentially-expressed in the SSH analysis, in seven tissues (muscle, gill, heart, hepatopancreas, brain, branchiostegite, gonad) dissected from individual symptomatic and asymptomatic lobsters. Expression of arginine kinase (involved in cellular energetics) was significantly decreased in muscle of symptomatic lobsters. Expression of hemocyanin (a respiratory hemolymph protein involved in oxygen transport) was highest in hepatopancreas and showed highly variable expression with a trend toward higher expression in asymptomatic individuals. 2-Macroglobulin (involved in the innate immune system) was most highly expressed in the ovary, particularly of symptomatic lobsters. The ESTs produced through this study add to the fledgling field of crustacean genomics and revealed three genes that could be further evaluated in lobsters of varying shell disease severity, molt stage, and reproductive condition, for possible implication in epizootic shell disease.

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Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Altered gene expression associated with epizootic shell disease in the American lobster, Homarus americanus

Tarrant

Stegeman

Verslycke

2010

Fish & Shellfish Immunology

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“…Because many forms of hemocyanin have been described in crustaceans, and some are upregulated after microbial challenge (Lei et al 2008, Tarrant et al 2010, it would be of interest to profile the expression patterns of additional forms of hemocyanin in future studies of lobsters with signs of disease. In the case of a2-macroglobulin, we have detected expression in ovary, and a2-macroglobulin does play a role in mammalian ovulation (Zhu & Woessner 1991); however, a2-macroglobulin is expressed primarily in hemocytes of other crustaceans and can be induced by microbial challenge (Rattanachai et al 2004, Lu et al 2008, Ho et al 2009. Thus, future studies should characterize expression of a2-macroglobulin and other components of the innate immune system in hemocytes in association with ESD.…”

Section: Discussionmentioning

confidence: 83%

Gene Expression in American Lobster (Homarus americanus) with Epizootic Shell Disease

Tarrant

Franks

Verslycke

2012

Journal of Shellfish Research

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Epizootic shell disease (ESD) has been reported widely in American lobster (Homarus americanus, Milne Edwards) in southern New England. The appearance of irregular, deep lesions-characteristic of ESD-has been associated previously with elevated levels of ecdysteroids and premature molting, but the underlying molecular and physiological changes associated with ESD remain poorly understood. Previously, we identified several genes, including arginine kinase and hemocyanin, that were expressed differentially in lobsters exhibiting signs of ESD (diseased) versus those lobsters exhibiting no signs of ESD (assumed healthy), and quantified their expression. In this study, we extend these findings and measure expression of a suite of 12 genes in tissues from 36 female lobsters of varying disease condition. In addition, molt stage is evaluated as a possible confounding factor in the expression of the selected genes. The expression of several genes changed significantly with disease stage. Arginine kinase expression decreased significantly in thoracic muscle of lobsters with signs of ESD. Ecdysteroid receptor expression was elevated significantly in both muscle and hepatopancreas of lobsters with signs of ESD. CYP45, a cytochrome P450 form that was shown previously to covary with ecdysteroid levels and to be inducible by some xenobiotics, showed significantly increased expression in hepatopancreas of lobsters with signs of ESD. Together, these results demonstrate that the expression of several genes is altered in lobsters showing signs of ESD, even when accounting for variation in molt stage. Given the observed changes in ecdysteroid receptor, arginine kinase, and CYP45 expression, further investigations of the association, if any, between molting, muscular function and xenobiotic metabolism and ESD are warranted.

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“…4). Other kuruma shrimp genes that are induced by PG include the genes for serine proteinase homolog (SPH) and a 2 -macroglobulin, and some antimicrobial peptides were reported to be induced after PG stimulation [28][29][30][31]. Even though kuruma shrimp SPH is also a member of the SP family, kuruma shrimp SPH differs from Mj-chy in that it has conserved His141, Asp187 and Gly285 residues for the catalytic triad, and three residues (Asn279, Gly308 and Gly318) that determine the substrate-binding pockets of trypsin in the SP-like domain.…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning, characterization and expression analysis of a chymotrypsin-like serine protease from kuruma shrimp Marsupenaeus japonicus

et al. 2009

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The mRNA encoding chymotrypsin-like serine protease (Mj-chy) from a kuruma shrimp Marsupenaeus japonicus hepatopancreas was identified as a peptidoglycan-inducible gene by 5 0 -end serial analysis of gene expression. The transcript of Mj-chy consists of a 816-nucleotide open reading frame encoding 271 amino acids, including a signal peptide of 17 amino acids and a trypsinlike serine protease domain of 219 amino acids. Like most serine proteases, Mj-chy has a catalytic triad consisting of histidine, aspartic acid and serine, and six cysteine residues forming three disulfide bridges. In a phylogenetic analysis, the trypsin-like serine protease domain clustered with invertebrate chymotrypsins and was closely related to chymotrypsin-like serine protease from Chinese shrimp Fenneropenaeus chinensis and chymotrypsin BI from Pacific white shrimp Litopenaeus vannamei. Mj-chy was detected in the hepatopancreas, stomach and intestine, and exhibited increased expression in defense-related tissues (i.e., hemocytes, lymphoid organ and hepatopancreas) after peptidoglycan stimulation.

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Molecular cloning and expression analysis of α2-macroglobulin in the kuruma shrimp, Marsupenaeus japonicus

Cited by 68 publications

References 41 publications

Altered gene expression associated with epizootic shell disease in the American lobster, Homarus americanus

Altered gene expression associated with epizootic shell disease in the American lobster, Homarus americanus

Gene Expression in American Lobster (Homarus americanus) with Epizootic Shell Disease

Molecular cloning, characterization and expression analysis of a chymotrypsin-like serine protease from kuruma shrimp Marsupenaeus japonicus

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