Molecular cloning and expression analysis of a new stress-related AREB gene from Arachis hypogaea

Abstract

An AREB gene, designated as AhAREB1, was cloned from peanut (Arachis hypogaea L.). The gene contains a 1 338-bp open reading frame that encodes a putative protein of 445 amino acids. The corresponding genomic DNA containing four exons and three introns was isolated and analyzed. An upstream 1 060-bp DNA promoter fragment of the AhAREB1 gene was also amplified from peanut genomic DNA. Multiple sequence alignment of the deduced amino acids of AREB showed that the AhAREB1 protein shares high sequence homology with GmAREB1, SlAREB, and ABF2. Quantitative real-time PCR analysis showed that AhAREB1 was induced by polyethylene glycol, NaCl, gibberellic acid, abscisic acid, and salicylic acid. The cloning and characterization of the AhAREB1 gene will be useful for further studies establishing the biological role of AhAREB1 in plants.

Negative feedback regulation of ABA biosynthesis in peanut (Arachis hypogaea): a transcription factor complex inhibits AhNCED1 expression during water stress

“…Both AhNAC2 and AhAREB1 genes are induced by ABA, but the protein expression pattern of the respective TFs is still unknown2627. The AhNAC2 mRNA level increased 3.5 times after 2 h ABA treatment (Supplementary Figure S1).…”

“…However, the positive feedback regulation of NCED3 expression by ABA raises the question of how the plant maintains ABA homeostasis during water stress. In previous work on peanut ( Arachis hypogaea ), an important cash crop, we cloned and identified NCED1 ( AhNCED1 ), NAC2 ( AhNAC2 ) and AREB1 ( AhAREB1 )42526. Furthermore, we showed that both AhNAC2 and AhAREB1 are induced by ABA in peanut, and that, in an Arabidopsis AhAREB1 -overexpression line, transcription of AtNCED3 is significantly suppressed2728.…”

Overexpression of Arachis hypogaea AREB1 Gene Enhances Drought Tolerance by Modulating ROS Scavenging and Maintaining Endogenous ABA Content

“…In our previous work, we have shown that AhAREB1 gene, which shares high sequence homology with GmAREB1 , SlAREB and ABF2/AREB1 , responds to environmental stimuli [18]. Further study showed that AhAREB1 functions as a transcriptional activator in yeast [19].…”

“…We have previously isolated a dehydration inducible AREB-like gene, AhAREB1 , from peanut and found that AhAREB1 gene was specifically induced by ABA, dehydration and drought [18]. Like others, AREBs/ABFs and AhAREB1 genes encode a transcription activator [19], and the amino acid sequence of AhAREB1 shared homology with other AREBs/ABFs [18]. However, the function and exact mechanisms of AhAREB1 in plant are still not clear.…”

Sucrose non-ferment 1 related protein kinase 2 (SnRK2) genes could mediate the stress responses in potato (Solanum tuberosum L.)

“…Systematic analysis of the plant cell network responsible for drought stress tolerance is a promising approach for the development of drought tolerant plants [1, 2]. Protein phosphorylation is involved in regulation of various cellular activities in plants and one of the main signals mediating the responses to environmental stresses [3–7]. The SnRKs (sucrose non-fermenting 1 related protein kinase) are a gene family coding for Ser/Thr protein kinases and play important roles in linking abiotic stress tolerance and the metabolic responses of plants [8–10].…”