Molecular Cloning and Expression Analysis of 13 MADS-Box Genes in Betula platyphylla

Abstract

MADS-box genes encode a family of transcription factors that control a diverse range of processes in flowering plants. In this study, 13 unique MADS genes were cloned from Betula platyphylla Suk., and 2-year-old Betula seedlings propagated in glasshouses were selected as plant materials. The expression profile of each BpMADS was investigated during the growth season and following gibberellin (GA) treatments by real-time quantitative reverse transcription polymerase chain reaction. The relative abundance of the 13 BpMADS was shown to differ during each month, indicating that the activity of the genes varies during the annual growing period. Expression analyses demonstrated that these BpMADS were regulated by GA signaling pathways. Furthermore, the variations in expression patterns suggest that the genes act independently to fulfill specific functions or act cooperatively in physiological processes. The study of birch MADSs is important for the purposes of improving breeding techniques and molecular biotechnology.

Diversity and Composition of Soil Acidobacterial Communities in Different Temperate Forest Types of Northeast China

“…Betula platyphylla Sukaczev. is light-loving and adaptable [50,51] but is more commonly found in moist soil conditions and is a pioneer species in secondary forests. Differences in the adaptation of above-ground vegetation to the environment in the two forest types led to variations in soil nutrient content, which, in turn, led to different OTUs of soil Acidobacteria specific to the different forest types.…”

AH2 encodes a MYB domain protein that determines hull fate and affects grain yield and quality in rice

“…hull). Conserved B and C genes specify petals/lodicules and stamens (Ambrose et al, 2000;Nagasawa et al, 2003;Whipple et al, 2004;Thompson et al, 2009;Li et al, 2012a;Sang et al, 2012;Wu et al, 2012). However, the developmental mechanisms that produce the lemma and palea remain unclear (Schmidt and Ambrose, 1998;Irish, 2000;Ferrario et al, 2004;Ohmori et al, 2009;Sang et al, 2012).…”