Molecular cloning and expression analysis of polymeric immunoglobulin receptor in flounder (Paralichthys olivaceus)

Adaptive immune responses at mucosal surfaces of teleost fish

“…In addition, it was shown that this tSC was associated with the gut mucus IgT and IgM. In olive flounder, a recombinant pIgR could interact with both mucus and serum IgM, and a flounder secretory component (fSC) could be detected in the skin mucus and not in the serum [55]. The molecular mass of fSC is around 37 kDa, which is also reported to be near the theoretical mass of the sequence of olive flounder pIgR [55].…”

“…The J-chain of mammals, birds and amphibians are all able to polymerize human IgA and IgM intracellularly while the J-chain of nurse shark, Ginglymostoma cirratum, cannot [51,54]. Till now a J chain has not been reported in any of the teleost species studied [55,56].…”

“…Upon release to the apical plasma membrane domain, the extracellular part of the receptor is cleaved off by a proteinase and co-secreted with the IgA or IgM as a protective secretory component (SC) [20,21]. The pIgR amino acid sequences of seven teleosts were published in the past decade: fugu [59], carp [60], orange-spotted grouper Epinephelus coioides [61], rainbow trout [35], zebrafish [62], Atlantic salmon [63] and olive flounder Paralichthys olivaceus [55]. The seven pIgR sequences were aligned along with the sequence of the Atlantic cod Gadus morhua pIgR.…”

Mucosal Immunoglobulins

“…The amino acid sequences of pIgR have been determined or deduced for several species and are shown in Chapter 19 (Eiffert et al, 1984(Eiffert et al, , 1991Mostov et al, 1984;Banting et al, 1989;Kulseth et al, 1995;Verbeet et al, 1995;Piskurich et al, 1995;Adamski and Demmer, 1999;Kumura et al, 2000;Taylor et al, 2002;Peters et al, 2003;Wieland et al, 2004;Rombout et al, 2008;Braathen et al, 2007;Hamuro et al, 2007;Rombout et al, 2008;Feng et al, 2009;Lewis et al, 2010;Xu et al, 2013). In addition to the intrachain disulfide bonds that are thought to be buried within each domain, several of the domains have "extra" disulfide bonds.…”

“…DNA sequence information for pIgR is now available from several other species, including human (Eiffert et al, 1984;Davidson et al, 1988;Krajci et al, 1989Krajci et al, , 1992, mouse (Piskurich et al, 1995), cattle (Kulseth et al, 1995;Verbeet et al, 1995), rat (Banting et al, 1989), horse (Lewis et al, 2010), dog (Peters et al, 2003), pig (Kumura et al, 2000), possum (Adamski and Demmer, 1999), wallaby (Taylor et al, 2002), chicken (Wieland et al, 2004), frog (Braathen et al, 2007), and various fishes (Hamuro et al, 2007;Rombout et al, 2008;Feng et al, 2009;Xu et al, 2013). Further sequences have been revealed in genome sequencing projects.…”