Molecular cloning and expression characterization of ApoC-I in the orange-spotted grouper

Wang, Y.; Zhou, Li; Li, Zhen; Gui, Jian‐Fang

doi:10.1007/s10695-007-9193-y

Cited by 16 publications

(10 citation statements)

References 53 publications

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“…This result is consistent with previous studies performed in mammals [90-92] and in another teleost, Hemibarbus mylodon [93]. Only in the orange-spotted grouper, apoC1 could not be detected in the liver but it was nonetheless expressed in gonad and in brain [94]. …”

Section: Discussionsupporting

confidence: 93%

Comparative transcriptomic analysis of follicle-enclosed oocyte maturational and developmental competence acquisition in two non-mammalian vertebrates

Gohin

Bobe²,

Chesnel

2010

BMC Genomics

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BackgroundIn vertebrates, late oogenesis is a key period during which the oocyte acquires its ability to resume meiosis (i.e. maturational competence) and to develop, once fertilized, into a normal embryo (i.e. developmental competence). However, the molecular mechanisms involved in these key biological processes are far from being fully understood. In order to identify key mechanisms conserved among teleosts and amphibians, we performed a comparative analysis using ovarian tissue sampled at successive steps of the maturational competence acquisition process in the rainbow trout (Oncorhynchus mykiss) and in the clawed toad (Xenopus laevis). Our study aimed at identifying common differentially expressed genes during late oogenesis in both species. Using an existing transcriptomic analysis that had previously been carried out in rainbow trout, candidate genes were selected for subsequent quantitative PCR-based comparative analysis.ResultsAmong the 1200 differentially expressed clones in rainbow trout, twenty-six candidate genes were selected for further analysis by real-time PCR in both species during late oogenesis. Among these genes, eight had similar expression profiles in trout and Xenopus. Six genes were down-regulated during oocyte maturation (cyp19a1, cyp17a1, tescalcin, tfr1, cmah, hsd11b3) while two genes exhibited an opposite pattern (apoc1, star). In order to document possibly conserved molecular mechanisms, four genes (star, cyp19a1, cyp17a1 and hsd11b3) were further studied due to their known or suspected role in steroidogenesis after characterization of the orthology relationships between rainbow trout and Xenopus genes. Apoc1 was also selected for further analysis because of its reported function in cholesterol transport, which may modulate steroidogenesis by regulating cholesterol bioavailability in the steroidogenic cells.ConclusionsWe have successfully identified orthologous genes exhibiting conserved expression profiles in the ovarian follicle during late oogenesis in both trout and Xenopus. While some identified genes were previously uncharacterized during Xenopus late oogenesis, the nature of these genes has pointed out molecular mechanisms possibly conserved in amphibians and teleosts. It should also be stressed that in addition to the already suspected importance of steroidogenesis in maturational competence acquisition, our approach has shed light on other regulatory pathways which may be involved in maturational and developmental competence acquisitions that will require further studies.

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Section: Discussionsupporting

confidence: 93%

Comparative transcriptomic analysis of follicle-enclosed oocyte maturational and developmental competence acquisition in two non-mammalian vertebrates

Gohin

Bobe²,

Chesnel

2010

BMC Genomics

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“…Then, RNAs were respectively reverse-transcribed with Goldscript cDNA synthesis kit (Invitrogen, Carlsbad, CA, USA) as described by the manufacturer. Semi-quantitative RT-PCR was performed as described previously 31 in a volume of 25 μ l as follows: 94 °C for 4 min, 94 °C for 30 s, 60 °C for 30 s and 72 °C for 1 min for 33 cycles followed by 72 °C for 10 min. Real-time quantitative PCR was performed by CFX96 Optics Module (Bio-Rad, Singapore) with SYBR Green I Dye as described previously.…”

Section: Methodsmentioning

confidence: 99%

RETRACTED ARTICLE: Zebrafish Noxa promotes mitosis in early embryonic development and regulates apoptosis in subsequent embryogenesis

Zhou

et al. 2014

Cell Death Differ

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Noxa functions in apoptosis and immune system of vertebrates, but its activities in embryo development remain unclear. In this study, we have studied the role of zebrafish Noxa (zNoxa) by using zNoxa-specifc morpholino knockdown and overexpression approaches in developing zebrafish embryos. Expression pattern analysis indicates that zNoxa transcript is of maternal origin, which displays a uniform distribution in early embryonic development until shield stage, and the zygote zNoxa transcription is initiated from this stage and mainly localized in YSL of the embryos. The zNoxa expression alterations result in strong embryonic development defects, demonstrating that zNoxa regulates apoptosis from 75% epiboly stage of development onward, in which zNoxa firstly induces the expression of zBik, and then cooperates with zBik to regulate apoptosis. Moreover, zNoxa knockdown also causes a reduction in number of mitotic cells before 8 h.p.f., suggesting that zNoxa also promotes mitosis before 75% epiboly stage. The effect of zNoxa on mitosis is mediated by zWnt4b in early embryos, whereas zMcl1a and zMcl1b suppress the ability of zNoxa to regulate mitosis and apoptosis at different developmental stages. In addition, mammalian mouse Noxa (mNoxa) mRNA was demonstrated to rescue the arrest of mitosis when zNoxa was knocked down, suggesting that mouse and zebrafish Noxa might have similar dual functions. Therefore, the current findings indicate that Noxa is a novel regulator of early mitosis before 75% epiboly stage when it translates into a key mediator of apoptosis in subsequent embryogenesis.

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“…Teleosts have been pointed out to possess more duplicate genes than mammals, which can be explained by fish-specific whole-genome duplication, additionally occurred after the divergence of ray-finned and lobe-finned fish, estimated around 250-400 million years ago [48][49][50]. Several apolipoprotein genes found in rainbow trout [19], Korean spotted barbell [22], pufferfish [4], orange-spotted grouper [51,52] and gibel carp [53] along with apoIA-Is in Japanese eel might have arisen in the fish evolutionary lineage. Different exchangeable apolipoproteins with lipid binding properties and diverse, specific functional domains seem to be associated with a complex transport system for the transfer of exogenous or endogenous lipids toward different tissues in fish.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of some novel genes of the apolipoprotein A-I family in Japanese eel, Anguilla japonica

et al. 2011

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Apolipoproteins such as apolipoprotein (apo) A-I, apoA-IV, and apoE are lipid binding proteins synthesized mainly in the liver and the intestine and play an important role in the transfer of exogenous or endogenous lipids through the circulatory system. To investigate the mechanism of lipid transport in fish, we have isolated some novel genes of the apoA-I family, apoIA-I (apoA-I isoform) 1–11, from Japanese eel by PCR amplification. Some of the isolated genes of apoIA-I corresponded to 28kDa-1 cDNAs which had already been deposited into the database and encoded an apolipoprotein with molecular weight of 28 kDa in the LDL, whereas others seemed to be novel genes. The structural organization of all apoIA-Is consisted of four exons separated by three introns. ApoIA-I10 had a total length of 3232 bp, whereas other genes except for apoIA-I9 ranged from 1280 to 1441 bp. The sequences of apoIA-Is at the exon-intron junctions were mostly consistent with the consensus sequence (GT/AG) at exon-intron boundaries, whereas the sequences of 3′ splice acceptor in intron 1 of apoIA-I1-7 were (AC) but not (AG). The deduced amino acid sequences of all apoIA-Is contained a putative signal peptide and a propeptide of 17 and 5 amino acid residues, respectively. The mature proteins of apoIA-I1-3, 7, and 8 consisted of 237 amino acids, whereas those of apoIA-I4-6 consisted of 239 amino acids. The mature apoIA-I10 sequence showed 65% identity to amino acid sequence of apoIA-I11 which was associated with an apolipoprotein with molecular weight of 23 kDa in the VLDL. All these mature apoIA-I sequences satisfied the common structural features depicted for the exchangeable apolipoproteins such as apoA-I, apoA-IV, and apoE but apoIA-I11 lacked internal repeats 7, 8, and 9 when compared with other members of apoA-I family. Phylogenetic analysis showed that these novel apoIA-Is isolated from Japanese eel were much closer to apoA-I than apoA-IV and apoE, suggesting new members of the apoA-I family.

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Molecular cloning and expression characterization of ApoC-I in the orange-spotted grouper

Abstract: Endogenous yolk nutrients are crucial for embryo and larval development in fish, but develop-

Cited by 16 publications

References 53 publications

Comparative transcriptomic analysis of follicle-enclosed oocyte maturational and developmental competence acquisition in two non-mammalian vertebrates

Comparative transcriptomic analysis of follicle-enclosed oocyte maturational and developmental competence acquisition in two non-mammalian vertebrates

RETRACTED ARTICLE: Zebrafish Noxa promotes mitosis in early embryonic development and regulates apoptosis in subsequent embryogenesis

Isolation and characterization of some novel genes of the apolipoprotein A-I family in Japanese eel, Anguilla japonica

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