Molecular Cloning and Expression Determination of p38 MAPK from the Liver and Kidney of Silver Carp

Abstract

The sequence of p38MAPK in silver carp (Hm-p38a) was cloned and sequenced. Additionally, the acute toxicity of crude microcystins (MCs) on silver carp and induction expression of Hm-p38a by MCs exposure were also determined in this study. The results reveal that the length of Hm-p38a is 2418 bp and it contains a 1086 bp open reading frame. Hm-p38a could encode 361 amino acids. Sequence analysis indicates that Hm-p38a contains the conserved structures of Thr-Gly-Tyr motif and substrate binding site Ala-Thr-Arg-Trp, and it is highly conserved in fish. Phylogenetic analysis reveals that Hm-p38a is more closely related to fish than mammals and it belongs to p38α subfamily. Moreover, Hm-p38a in silver carp is constitutively expressed in all examined tissues. In addition, our results indicate that MCs exposure significantly promotes the transcription of Hm-p38a in fish liver or kidney, suggesting that mitogen-activated protein kinase should also be the signal pathway of MCs hepatotoxicity in fish.

Antibacterial ability of two species of tilapia is related to inducibility of the p38 mitogen‐activated protein kinase pathway

“…In previous studies, the p38 MAPK pathway in grouper was shown to be activated by infection with Singapore grouper iridovirus (SGIV) (Cai et al., 2011; Huang et al., 2011). Other studies have shown that bacterial and viral infection can activate the p38 MAPK signalling pathway in various fish (Li et al., 2016; Umasuthan et al., 2015; Zhu, et al., 2014a). In this study, both gene and protein expressions of p38 MAPK were significantly increased in Nile tilapia and hybrid tilapia after S. agalactiae infection, and the abundance of the p38 MAPK protein increased in a time‐dependent manner.…”

“…However, research on the roles of p38 MAPKs in the pathogenesis of fish is limited. To date, p38 MAPKs have been detected in carp (Hashimoto et al., 2000), grass carp (Li et al., 2015a), silver carp (Li et al., 2016; Ma et al., 2018), zebrafish (Krens et al., 2006), Atlantic salmon (Hansen & Jørgensen, 2007), orange‐spotted grouper (Cai et al., 2011), amphioxus (Zhu et al., 2014a), blunt snout bream (Zhang et al., 2018) and large yellow croaker (Li et al., 2019b). A few kinds of parasites, bacteria and viruses could active p38 MAPK pathway, such as Aeromonas hydrophila , Microcystins and Singapore grouper iridovirus .…”

Analysis of MicroRNA Expression Profiling Involved in MC-LR-Induced Cytotoxicity by High-Throughput Sequencing

“…Early in 1999, Toivola and Eriksson hypothesized that MC might promote MAPK signaling pathways and MAPK be a key pathway for MCs-toxicity [ 44 ]. In recent years, more and more studies confirmed that MC-hepatotoxicity was mediated by MAPK pathway due to PP2A inhibition by MC [ 45 , 46 , 47 , 48 ]. In the present study, considerable target genes of aberrant miRNAs induce by MC-LR were also enriched to MAPK pathway, suggesting that the pathway may play a key role in MC-LR cytotoxicity on HepG2 cells via the negative regulation of miRNAs.…”

Hypoxia Stress Induces Tissue Damage, Immune Defense, and Oxygen Transport Change in Gill of Silver Carp (Hypophthalmichthys molitrix): Evaluation on Hypoxia by Using Transcriptomics

“…The genes utilized to validate the RNA-seq data were the members of overlapping DEGs from CK versus T1, CK versus T2, and CK versus T3, and their primers were built using Primer 6. B-actin served as the control gene (Li et al, 2013;Li et al, 2016). qPCR was performed on samples that were not related to those utilized for RNA-seq.…”