Molecular cloning and expression of the hot pepper ERabp1 gene encoding auxin-binding protein

Choi, So Yoon

doi:10.1007/bf00020496

Cited by 13 publications

(5 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…10) of total protein showed a highest level at the post-veraison rapid expanding stage (about 80 days after full bloom), less ABP1 protein was found at veraison stage (about 70 days after full bloom) and ripening stage (90-120 days after full bloom), whereas scarcely in fruits at the early developmental stage (30-50 days after full bloom). This result was similar to Choi (1996), which showed that the Ca-ERabp1 gene was highly expressed at middle stage of hot pepper fruit development. It is interesting that the expression of ABP1 was restricted to young grape berries.…”

Section: Expression Of Abp1 In Fruit Developmentsupporting

confidence: 92%

cDNA Cloning, Prokaryotic Expression, Polyclonal Antibody Preparation of the Auxin-Binding Protein 1 Gene from Grape Berry

et al. 2010

View full text Add to dashboard Cite

Auxin-binding protein 1 (ABP1) plays an important role in the growth and development of plants. In this study, a novel grape ABP1 cDNA was isolated by reverse transcription polymerase chain reaction, using in silico cloning strategy based on grape dbEST. The obtained grape ABP1 cDNA is 756 bp containing a 567 bp open reading frame, which encodes a 188 amino acid protein. The deduced amino acid sequence possessed all of the three typical domains of plant ABP1s, an amino-terminal signal peptide and a carboxyl-terminal sorting signal, KDEL. A full-length ABP1 cDNA was introduced into an expressed plasmid pET-30a(+) vector at the EcoR I and Xho I restriction sites. The pET-ABP1 was found to be highly expressed in Escherichia coli BL21(DE3) pLysS cells with isopropyl-β-D-thiogalactoside induction. A fusion protein was purified and used as the antigen to immunize a New Zealand rabbit. The resulting antiserum was then further purified by HiTrap rProtein A FF Affinity Purification Kit to obtain the immunoglobulin G fraction. Western blot analysis indicated that ABP1 was regulated in fruits depending on the developmental stage. Our work represented a first step towards a better understanding of function analysis of grape ABP1.

show abstract

Section: Expression Of Abp1 In Fruit Developmentsupporting

confidence: 92%

cDNA Cloning, Prokaryotic Expression, Polyclonal Antibody Preparation of the Auxin-Binding Protein 1 Gene from Grape Berry

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Recent results indicated that auxin-dependent cell expansion is mediated by overexpressed ABP1 (Jones et al 1998), which provides the direct in vivo evidence that ABP is a functional auxin receptor. In the last 10 yr, ABP genes have been isolated from a number of plant species (Anai et al 1997;Choi 1996;Hesse et al 1989;Inohara et al 1989;Lazarus and Macdonald 1996;Leblanc et al 1997;Palme et al 1992;Watanabe and Shimomura 1998), but none of them have been cloned from herbicideresistant plants. Webb and Hall (1995) studied the effects of various auxinic herbicides on the binding of [ 3 H]IAA to ABP preparations and seedling growth and hypothesized that herbicide sensitivity and morphological differences between R and S biotypes may be due to the interaction of auxinic herbicides with ABP or other auxin receptors.…”

Section: Abp and Auxinic Herbicide Resistance In Wild Mustardmentioning

confidence: 99%

Understanding auxinic herbicide resistance in wild mustard: physiological, biochemical, and molecular genetic approaches

Zheng

Hall²

2001

Weed Science

View full text Add to dashboard Cite

The incidence of auxinic herbicide resistance in plants has increased worldwide. Auxinic herbicides were the first selective organic herbicides developed and have been used in agriculture for over 50 yr, primarily for the selective control of broadleaf weeds in cereal crops. However, the mode of action of auxinic herbicides and the molecular basis of auxinic herbicide resistance remain unknown, although an auxin-binding protein (ABP) is proposed to be the primary target site. Using auxinic herbicide-resistant (R) and -susceptible (S) biotypes of wild mustard as a model system, we have extensively studied the mode of action of auxinic herbicides and the resistance mechanisms at the physiological, biochemical, and molecular genetic levels. There are no differences in uptake, transport, and metabolism of auxinic herbicides between the R and S biotypes. Based on these results, as well as the studies on the role of auxin-enhanced ethylene biosynthesis and calcium in mediating the auxinic herbicide resistance, we hypothesize that resistance of the R biotype to auxinic herbicides is due to an altered target site, possibly an auxin receptor. We have identified and characterized a small ABP gene family as well as their cDNAs from both R and S of wild mustard. Amino acid changes were found in the ABP of the R biotype. Functional and mutational analyses of these genes are underway to determine the role of ABP in mediating auxinic herbicide resistance. In this review, we focus on the mode of action of auxinic herbicides and the molecular basis of auxinic herbicide resistance in wild mustard.

show abstract

“…ABP1 is a soluble protein first isolated from maize coleoptiles [5, 6]. Genomic or cDNA clones of ABP1 have been isolated from different plant species such as maize [6–8], Arabidopsis [9, 10], tobacco [11, 12], strawberry [13], red pepper [14]or apple tree [15]. All of the deduced amino acid ABP1 sequences possess a leader peptide at the N‐terminus of the protein and a KDEL (Lys‐Asp‐Glu‐Leu) motif at the C‐terminus.…”

Section: Introductionmentioning

confidence: 99%

The auxin‐binding protein Nt‐ERabp1 alone activates an auxin‐like transduction pathway

1999

View full text Add to dashboard Cite

Hyperpolarization of tobacco protoplasts is amongst the earliest auxin responses described. It has been proposed that the auxin-binding protein, ABP1, or a related protein could be involved in the first step of auxin perception at the plasma membrane. Using for the first time homologous conditions for interaction between the protein Nt-ERabp1 or a synthetic peptide corresponding to the C-terminus and tobacco protoplasts, we have demonstrated that both can induce the hyperpolarization response. The results show that Nt-ERabp1 or the C-terminal peptide alone activates the auxin pathway from the outer face of the plasma membrane.z 1999 Federation of European Biochemical Societies.

show abstract

Molecular cloning and expression of the hot pepper ERabp1 gene encoding auxin-binding protein

Cited by 13 publications

References 19 publications

cDNA Cloning, Prokaryotic Expression, Polyclonal Antibody Preparation of the Auxin-Binding Protein 1 Gene from Grape Berry

cDNA Cloning, Prokaryotic Expression, Polyclonal Antibody Preparation of the Auxin-Binding Protein 1 Gene from Grape Berry

Understanding auxinic herbicide resistance in wild mustard: physiological, biochemical, and molecular genetic approaches

The auxin‐binding protein Nt‐ERabp1 alone activates an auxin‐like transduction pathway

Contact Info

Product

Resources

About