Molecular cloning and expression of bovine nucleoplasmin 2 (NPM2): a maternal effect gene regulated by miR-181a

Lingenfelter, Brandon M.; Tripurani, Swamy K.; Tejomurtula, Jyothsna; Smith, George W.; Yao, Jianbo

doi:10.1186/1477-7827-9-40

Cited by 52 publications

(62 citation statements)

References 39 publications

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“…The injection of NPM into the bovine oocyte after fertilisation results in a significant improvement in the rate of pregnancy (Betthauser et al 2006). This suggests that the role of NPM is extended to reprogramming the somatic nucleus (Lingenfelter et al 2011). Conversely, the common regulation system that down-regulates gene expression is associated with microRNAs (miRNA) and pre-microRNAs (miR).…”

Section: Molecular Markers Of Oocyte Developmental Competencementioning

confidence: 99%

Developmental competence of mammalian oocytes-insights into molecular research and the promise of microfluidic technology: a review

Kempisty¹,

Zawierucha²,

Ciesiółka³

et al. 2015

Vet. Med. - Czech

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Developmental competence of female gametes determines their maturation ability, successful fertilisation, and proper zygote formation. Oocyte quality may be assessed by expression profiling of several gene markers such as Cx43, TGFB, GDF9, BMP, Lox and Pdia5 that determine the biological features of oocytes. Conversely, several other extrinsic factors, including follicular size or heat shock may significantly influence oocyte quality and ability to grow and develop during folliculo-and oogenesis. However, using molecular methods for evaluation of oocyte quality often leads to destruction of an analysed cell. Therefore, there is an increased requirement to seek new non-invasive methods of oocyte-embryo quality assessment. Here we describe the Lab-on-Chip system based on microfluidic technology, which is the first parametric and objective device for evaluation of oocyte developmental competence using spectral images. In this review several extrinsic factors and molecular markers of oocyte developmental competence are discussed. Furthermore, based on our previous studies, we discuss the possibility of applying the spectrophotometric system (Lab-on-Chip) in both biomedical and reproductive research in domestic animals.

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Section: Molecular Markers Of Oocyte Developmental Competencementioning

confidence: 99%

Developmental competence of mammalian oocytes-insights into molecular research and the promise of microfluidic technology: a review

Kempisty¹,

Zawierucha²,

Ciesiółka³

et al. 2015

Vet. Med. - Czech

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“…The action of miRNAs on maternal mRNA degradation has been clear in Zebrafish embryos, which shows that the miR430 is abundant in the embryonic activation point, just at the moment the degradation of the maternal transcripts takes place (44) . Recent studies have shown that in the bovine, maternal mRNA as NOBOX and NPM2 are regulated under the action of the miR-181st and miR196a, respectively (104,105) . It is important to point out that the route of the degradation mediated by miRNAs is present only during the activation of the embryonic genome, and is absent during the oogenesis and in the initial stages of the embryo (47,52,54,106) .…”

Section: Maternal Effect On the Activation Of The Embryonic Genomementioning

confidence: 99%

“…La acción de miRNAs sobre la degradación de RNAm maternos ha quedado clara en embriones del Zebrafish, donde se observa que el miR430 es abundante en el punto de la activación embrionaria, justo en el momento en que sucede la degradación de los transcritos maternos (44) . Estudios recientes han demostrado que en el bovino, RNAm maternos como NOBOX y NPM2 son regulados bajo la acción de los miR-181a y miR196a, respectivamente (104,105) . Es importante señalar que la vía de degradación mediada por los of mice brg1-/-are arrested at the 2-4 cells stage (108) .…”

Section: Efecto Materno Sobre La Activación Del Genoma Embrionariounclassified

“…This indicates that NMP2 may be required for the nuclear organization during the activation of the embryonic genome. The transcript NMP2 has a binding site on its UTR´3 end for the miR-181st and experiments in cattle showed that the translation of NPM2 is suppressed by this miRNA (104) .…”

Section: Rnam Maternos En El Bovinomentioning

confidence: 99%

“…Esto indica que NMP2 podría ser requerida para la organización nuclear durante la activación del genoma embrionario. El transcrito de NMP2 tiene un sitio de unión en su extremo UTR´3 para el miR-181a y en experimentos en bovino se demostró que la traducción de NPM2 es reprimida por este miRNA (104) .…”

Section: Rnam Maternos En El Bovinounclassified

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Efectos de los RNAm maternos sobre la maduración del ovocito y el desarrollo embrionario temprano en mamíferos

Burrola-Barraza¹,

González-Rodríguez

2015

Rev. Mex. Cienc. Pecu.

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Efectos de los RNAm maternos sobre la maduración del ovocito y el desarrollo embrionario temprano en mamíferos. RevisiónEffects of the maternal mRNA on the maturation of the oocyte and early embryonic development in mammals. Review M. Eduviges Burrola-Barraza a , Everardo González-Rodríguez a RESUMENObtener ovocitos madurados in vitro, que sean competentes para fertilizarse y originar un porcentaje superior al 50 % de blastocitos viables, es una de las principales metas que existen en el desarrollo in vitro de embriones bovinos. Es por ello necesario entender los procesos celulares, que desembocan en la maduración del ovocito durante la foliculogénesis y su subsecuente transición al embrión. La transición del ovocito al embrión es un proceso complejo que involucra la inactivación genómica del ovocito y la activación del genoma embrionario. Este proceso se da en bovinos en la etapa de 8 a 16 células y presenta una degradación selectiva de RNAm maternos, que fueron almacenados durante la ovogénesis y son la fuente para la codificación de proteínas en las etapas iníciales del desarrollo embrionario. La acción de los RNAm maternos es clave para que la activación del genoma embrionario se realice en tiempo y forma adecuados. Entender la participación de estos transcritos en la activación del genoma embrionario, es esencial para esclarecer los procesos celulares que fallan en los protocolos de maduración de ovocitos in vitro. Es por esto que el objetivo de esta revisión fue recopilar la información de los principales RNAm maternos que han sido identificados tanto en el modelo murino como el bovino, esto con el fin de integrar el conocimiento relacionado con los procesos genómicos que permiten el desarrollo del embrión en el bovino, y diseñar nuevas estrategias que permitan mejorar los protocolos de fertilización in vitro.PALABRAS CLAVE: Ovocito, Activación genoma embrionario, Bovino. ABSTRACTGet in vitro matured oocytes that could be competent for in vitro fertilization with a result of 50 % viable blastocysts, is one of the main problems in the in vitro development of bovine embryos. For these reason, is necessary to understand the cellular processes that lead to the maturation of the oocyte during folliculogenesis and subsequent transition to the embryo. The transition from oocyte to embryo is a complex process that involves genomic inactivation of the oocyte and embryonic genome activation. This process occurs in cattle in the stage of 8 to 16 cells where there are a selective degradation of maternal mRNA, which were stored during oogenesis and are the source of protein in the early stages of embryonic development. The action of maternal mRNA is a key for that embryonic genome activation takes place in form and time. Understanding the involvement of these transcripts in embryonic genome activation is essential to elucidate the cellular processes falling in oocyte maturation in vitro protocols. The aim of this review was to gather information from maternal genes that have been isolated both in the murine model and c...

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Transcriptome analysis of bovine oocytes from distinct follicle sizes: Insights from correlation network analysis

Labrecque

Fournier

Sirard

2016

Molecular Reproduction Devel

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Follicle size is recognized as a predictor of the potential for the enclosed oocyte to yield an embryo following in vitro maturation and in vitro fertilization. Oocytes from larger follicles are more likely to reach the blastocyst stage than those from smaller follicles. A growing oocyte accumulates all the transcripts needed to ensure development until the maternal embryonic transition, and this accumulation must be completed before the period of transcriptional arrest. Accordingly, the transcriptomes of bovine germinal-vesicle-stage oocytes collected from follicles of increasing sizes (<3, 3-5, >5-8, and >8 mm) were evaluated, using the EmbryoGENE bovine transcriptomic platform (custom Agilent 4 × 44 K), to better understand transcriptional modulation in the oocyte as the follicle becomes larger. Microarray analyses revealed very few differences between oocytes from small follicles (<3 vs. 3-5 mm), whereas an important number of differences were detected at the mRNA level between oocytes from larger follicles. Weighted gene correlation network analysis allowed for the identification of several hub genes involved in crucial functions such as transcriptional regulation (TAF2), chromatin remodeling (PPP1CB), energy production (SLC25A31), as well as transport of key molecules within the cell (NAGPA, CYHR1, and SLC3A12). The results presented here thus reinforce the hypothesis that developmental competence acquisition cannot be seen as a simple one-step process, especially in regards to the modulation of mRNA. Mol. Reprod. Dev. 83: 558-569, 2016. © 2016 Wiley Periodicals, Inc.

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Molecular cloning and expression of bovine nucleoplasmin 2 (NPM2): a maternal effect gene regulated by miR-181a

Cited by 52 publications

References 39 publications

Developmental competence of mammalian oocytes-insights into molecular research and the promise of microfluidic technology: a review

Developmental competence of mammalian oocytes-insights into molecular research and the promise of microfluidic technology: a review

Efectos de los RNAm maternos sobre la maduración del ovocito y el desarrollo embrionario temprano en mamíferos

Transcriptome analysis of bovine oocytes from distinct follicle sizes: Insights from correlation network analysis

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