Molecular cloning and expression of i-type lysozyme and phage-type like lysozyme gene from freshwater shellfish

Abstract

Lysozyme is a widely distributed hydrolase possessing lytic activity against bacterial peptidoglycan, which enables it to protect the host against pathogenic infection. In the present study, A i-type lysozyme, designated as CpLYZ3, from Cristaria plicata and a phage-type lysozyme, designated as HcPLYZ, from Hyriopsis cumingii were identified by rapid amplification of cDNA ends and nested PCR. The