Molecular cloning and expression of a Penicillium citrinum allergen with sequence homology and antigenic crossreactivity to a hsp 70 human heat shock protein

SHEN, H.-D.; AU, L.-C.; LIN, W.-L.; LIAW, S.-F.; TSAI, J.-J.; HAN, S.-H.

doi:10.1046/j.1365-2222.1997.d01-546.x

Cited by 10 publications

(11 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, eleven heat shock protein 70 (Hsp70) (Spots 4, 5, 7, 8, 9, 10, 11, 12, 13, 21 and 31) and four small Hsps (spots 172, 173, 217 and 218) were identified as corresponding to allergenic molecules. Hsp70 have been demonstrated to bind to human IgE from allergenic patients to cystic echinococcosis [ 29 ], corn and barley [ 30 ], and antigenic cross-activity of Hsp70 with a 70 kDa component was proved by amino acid sequence alignment in Penicillium citrinum [ 31 ]. Class I small heat shock protein (Hsp) detected on a 2D gel have reported that it is one of allergens in soybean [ 32 ].…”

Section: Resultsmentioning

confidence: 99%

Proteomic Analysis and Identification of Possible Allergenic Proteins in Mature Pollen of Populus tomentosa

Rui

Zhang

Fan

et al. 2018

IJMS

View full text Add to dashboard Cite

Pollen grains from Populus tomentosa, a widely cultivated tree in northern area of China, are considered to be an important aeroallergen causing severe allergic diseases. To gain insight into their allergenic components, mature Populus tomentosa pollen proteins were analyzed by two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF/TOF MS). A total of 412 spots from mature pollen were resolved on pH 4–7 immobilized pH gradient (IPG) strips and 159 distinct proteins were identified from 242 spots analyzed. The identified proteins were categorized based on their functional role in the pollen, which included proteins involved in energy regulation, protein fate, protein synthesis and processing, metabolism, defense/stress responses, development and other functional categories. Moreover, among the identified proteins, 27 proteins were identified as putative allergens using the Structural Database of Allergenic Proteins (SDAP) tool and Allergen Online. The expression patterns of these putative allergen genes indicate that several of these genes are highly expressed in pollen. The identified putative allergens have the potential to improve specific diagnosis and can be used to develop vaccines for immunotherapy against poplar pollen allergy.

show abstract

Section: Resultsmentioning

confidence: 99%

Proteomic Analysis and Identification of Possible Allergenic Proteins in Mature Pollen of Populus tomentosa

Rui

Zhang

Fan

et al. 2018

IJMS

View full text Add to dashboard Cite

show abstract

“…It has reported that class I small heat shock protein (Hsp) is one of allergens in soybean (Gagnon et al, 2010 ). Hsp70 proteins have been demonstrated to bind to human IgE from patients sensitized to penicillium (Shen et al, 1997 ), cystic echinococcosis (Ortona et al, 2003 ), and to corn and wheat dust (Chiung et al, 2000 ). Spots No.…”

Section: Resultsmentioning

confidence: 99%

Proteomic analysis and candidate allergenic proteins in Populus deltoides CL. “2KEN8” mature pollen

Zhang

Fan

et al. 2015

Front. Plant Sci.

View full text Add to dashboard Cite

Proteomic analysis was used to generate a map of Populus deltoides CL. “2KEN8” mature pollen proteins. By applying 2-D electrophoresis, we resolved 403 protein spots from mature pollen. Using the matrix-assisted laser desorption/ionization time time-of-flight/time-of-flight tandem mass spectrometry method, we identified 178 distinct proteins from 218 protein spots expressed in mature pollen. Moreover, out of these, 28 proteins were identified as putative allergens. The expression patterns of these putative allergen genes indicate that several of these genes are highly expressed in pollen. In addition, the members of profilin allergen family were analyzed and their expression patterns were compared with their homologous genes in Arabidopsis and rice. Knowledge of these identified allergens has the potential to improve specific diagnosis and allergen immunotherapy treatment for patients with poplar pollen allergy.

show abstract

“…Bioinformatic analysis using Allertop, PREAL, and ALGPRED predicted that Aed a 8 is an allergen (Table ). Multiple sequence alignment between Aed a 8 and HSP‐70 protein from Dermatophagoides pteronyssinus , Cladosporium herbarum , and Penicillium citrinum showed that the overall sequences are highly conserved (Data not shown). Aed a 5 was predicted as an allergen by Algpred and PREAL and Aed a 6 by Allertop and Algpred (Table ).…”

Section: Resultsmentioning

confidence: 99%

Allergenome characterization of the mosquito Aedes aegypti

Cantillo

Puerta

Puchalska

et al. 2017

Allergy

View full text Add to dashboard Cite

show abstract

Molecular cloning and expression of a Penicillium citrinum allergen with sequence homology and antigenic crossreactivity to a hsp 70 human heat shock protein

Abstract: Results obtained suggest that hsp 70 is an allergen of P. citrinum and that clones SC4 and XL contain partial cDNAs of this allergen gene.

Cited by 10 publications

References 21 publications

Proteomic Analysis and Identification of Possible Allergenic Proteins in Mature Pollen of Populus tomentosa

Proteomic Analysis and Identification of Possible Allergenic Proteins in Mature Pollen of Populus tomentosa

Proteomic analysis and candidate allergenic proteins in Populus deltoides CL. “2KEN8” mature pollen

Allergenome characterization of the mosquito Aedes aegypti

Contact Info

Product

Resources

About