Molecular cloning and expression of peptidoglycan recognition protein (PGRPs) gene from freshwater mussel Hyriopsis cumingii

Hu, Baoqing; Zeng, Mingyu; Dai, Wenjuan; Wen, Can; Liu, Yi

doi:10.1016/j.fsi.2016.04.095

Cited by 2 publications

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“…Functional analysis revealed that HcPGRP2 exhibits various immune-related activities, including binding to peptidoglycan (PGN), microbial binding, antibacterial activity, and regulation of immune genes. These ndings suggest that HcPGRP2 may play a role in recognizing and eliminating pathogens through its bacterial recognition and effector functions [13].Another study identi ed two PGRPs in the mollusk Solen grandis, termed SgPGRP-S1 and SgPGRP-S2, which are secreted proteins lacking transmembrane domains [13].which is consistent with other PGRPs identi ed from mollusks, such as CfPGRP-S1, AiPGRP, and CgPGRPs [11,20]. All the PGRPs identi ed from mollusks so far are PGRP-S, suggesting a PGRP-mediated immune defense mechanism probably different from those in Drosophila and Homo sapiens.…”

Section: Introductionmentioning

confidence: 88%

Cloning of Pgrp Gene and its Expression Responding to the Stimulations Of Vibrio Alginolyticus, Lps, and Poly (I:C) in Pinctada Fucata

Zhang,

Yu,

Liang

et al. 2023

Preprint

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Section: Introductionmentioning

confidence: 88%

Cloning of Pgrp Gene and its Expression Responding to the Stimulations Of Vibrio Alginolyticus, Lps, and Poly (I:C) in Pinctada Fucata

Zhang,

Yu,

Liang

et al. 2023

Preprint

View full text Add to dashboard Cite

Cloning of PGRP Gene and Its Expression Responding to the Stimulations of Vibrio Alginolyticus, LPS, and Poly (I:C) in Pinctada fucata

Zhang,

Yu,

Liang

et al. 2023

Preprint

View full text Add to dashboard Cite

The PfPGRP gene from the pearl oyster Pinctada fucata was cloned and expressed in different tissues with and without stimulation by Vibrio alginolyticus, LPS, and Poly (I:C) to investigate its role in the immune response. The cDNA sequence of PfPGRP was 789 bp long, containing an open reading frame of 591 bp that encoded 196 amino acids. Phylogenetic analysis showed that the genetic distance between vertebrate and invertebrate PGRPs was significant, and the homology of PGRP gene sequences in mollusks was around 50%. The PfPGRP gene had the highest homology with the PGRP gene from Crassostrea gigas, at 72.9%. Protein domain prediction identified a conserved canonical PGRP domain and an Ami_2 domain in PfPGRP, suggesting that it could hydrolyze invading bacteria and terminate the immune response. Quantitative PCR showed that PfPGRP was expressed constitutively in all tissues, with the highest levels in the mantle, followed by the gonad and hepatopancreas. The expression of PfPGRP in the mantle was significantly upregulated after LPS injection, indicating that this tissue is more sensitive to LPS and plays a crucial role in defending against bacterial invasions. Similarly, the expression of PfPGRP in the hepatopancreas was significantly upregulated after both Vibrio and LPS injections, but reached its maximum later in the Poly (I:C) group, suggesting that it is more sensitive to bacteria and may be involved in bacterial elimination.Overall, these results suggest that PfPGRP plays an essential role in the innate immune system of P. fucata and is involved in defense against bacterial and viral invasions.

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Molecular cloning and expression of peptidoglycan recognition protein (PGRPs) gene from freshwater mussel Hyriopsis cumingii

Cited by 2 publications

References 0 publications

Cloning of Pgrp Gene and its Expression Responding to the Stimulations Of Vibrio Alginolyticus, Lps, and Poly (I:C) in Pinctada Fucata

Cloning of Pgrp Gene and its Expression Responding to the Stimulations Of Vibrio Alginolyticus, Lps, and Poly (I:C) in Pinctada Fucata

Cloning of PGRP Gene and Its Expression Responding to the Stimulations of Vibrio Alginolyticus, LPS, and Poly (I:C) in Pinctada fucata

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