Molecular cloning and expression of hepatopancreas glutamine synthetase in the Pacific white shrimp, Litopenaeus vannamei, induced by acute hypo-osmotic stress

Liu, Hongyu; Sun, Wu-wei; Tan, Beiping; Chi, Shuyan; Dong, Xiaohui

doi:10.1016/j.aquaculture.2012.07.031

Cited by 30 publications

(25 citation statements)

References 49 publications

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“…Also, peroxiredoxin expression levels increased in mole cricket Gryllotalpa orientalis and Bombyx mori under oxidative stress induced by temperature Lee et al 2005). The expression of hepatopancreas glutamine synthetase (GS), which is a key enzyme involved in nitrogen metabolism and glutamine synthesis in the Pacific white shrimp, Litopenaeus vannamei, induced by acute hypo-osmotic stress was studied by Liu et al (2012) through qRT-PCR analysis. Results showed that glutamine synthetase was expressed in the stomach, gill, intestine, hepatopancreas, muscle, heart and eyestalk and up-regulated in the stomach, gills, intestines and hepatopancreas after 48 h of exposure to dilute sea water (4 ppt).…”

Section: Osmotic Stressmentioning

confidence: 99%

Transcriptional stress responses to environmental and husbandry stressors in aquaculture species

Eissa

Wang

2014

Reviews in Aquaculture

116

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Stress of aquatic animals occurs due to physical and physiological disturbances in the aquatic environment or system when transportation, crowding, handling or changes of physical and chemical factors take place. There are three regulatory systems having a vital role in stress response: the neural, endocrine and immune systems. Fish exhibit multiple genomic and physiological responses to adjust the compensatory or adaptive mechanism that allows them to mitigate the stressors, maintain their haemostasis and survive. In this review, we describe multiple important genes that are associated with responses to environmental and husbandry stressors and that could be used as biomarkers of environmental and husbandry stressors in fish. The described environmental and husbandry stressors include salinity, temperature, hypoxia and hyperoxia, confinement, density and handling. The main role of stress response in aquatic animals is to compensate or adapt their biological systems and arrange the metabolism to afford the energy required by the stressor and a wide array of metabolic processes and pathways are involved. We summarized and discussed highly significant genes in several organs and tissues that are involved and active during this adaption process. The traditional stress biomarkers in some circumstances have some difficulties in interpretation of results and lead to tricky diagnosis and searching and understanding alternative tools is critical for aquaculture, fisheries and fish welfare. Using genomic tools to study the candidate genes associated with stress responses are often unique signatures or imprints of specific stressors and could determine early signs of stressors.

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Section: Osmotic Stressmentioning

confidence: 99%

Transcriptional stress responses to environmental and husbandry stressors in aquaculture species

Eissa

Wang

2014

Reviews in Aquaculture

116

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“…In crustaceans, major part of nitrogen is excreted ammonia, amino acid as well as urea and uric acid (in small amounts) formations (Lee & Chen, 2003). The haemolymph urea and uric acid levels in Marsupenaeus japonicus (Cheng & Chen, 1998;Cheng et al, 2004), Penaeus monodon (Cheng & Chen, 2002) and L. vannamei (Liu et al, 2012) were altered when shrimp exposed to different environmental conditions. We find that the ACV and PA supplemental diets had no effect on the levels of urea, uric acid and creatinine.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of some haemolymph biochemical properties and F‐cell prevalence in hepatopancreas of white leg shrimp (Litopenaeus vanammei) after fed diets containing apple cider vinegar and propionic acid

et al. 2019

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The present study aimed to evaluate the effect of dietary ACV (apple cider vinegar) and PA (propionic acid) supplementation on some biochemical properties of haemolymph and fibrillar cell (F‐cell) number in the hepatopancreas of shrimp. Five different diets supplemented with 0%, 1%, 2%, 4% ACV and 0.5% PA diets were fed to Litopenaeus vanammei. Some biochemical parameters of haemolymph (cortisol, alkaline phosphatase, albumin, urea, uric acid, creatinine, phosphorus, total hemocyte count) and prevalence of F‐cell in hepatopancreas were examined after 60 days feeding trial. The albumin concentration and total hemocyte count in 2% and 4% ACV and PA groups were significantly higher than those of other groups (p < .05). Moreover, the highest phosphorus concentration was detected in shrimp fed 4% ACV and PA experimental diets (p < .05). No significant differences in haemolymph cortisol, urea, uric acid, creatinine, alkaline phosphatase and tubule area were observed among the experimental groups (p > .05). 2% ACV group showed more F‐cell number in hepatopancreas than other groups while the reduction in F‐cell number was observed in the PA group. Based on these observations, the tested ACV and PA diets remarkably exhibited positive impacts on nutritional value and they may be a potential for adding to shrimp diet.

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“…Many of these genes have been shown to be important for adaptation in response to salinity gradients and also in stress response to abrupt changes in pH or water chemistry (Henry et al, 2002;Serrano and Henry, 2008;Liu et al, 2010;Havird et al, 2013). One group of candidate genes of particular interest are those involved in ion transport and systemic acid-base balance.…”

Section: Other Candidate Osmoregulatory Genes In C Quadricarinatusmentioning

confidence: 99%

“…Among these, CA is one of the master genes that has been reported to be involved in acidbase regulation in a wide variety of marine crustacean taxa (e.g. shrimp: (Gao and Wheatly, 2004;Pongsomboon et al, 2009;Liu et al, 2010;Pan et al, 2011;Hu et al, 2013); crab: (Serrano et al, 2007;Serrano and Henry, 2008;Liu et al, 2012)). Carbonic anhydrases also play vital role in osmoregulation because they provide H + and HCO 3 − ions which are used as counterions for exchanging cations and anions, especially Na + and Cl − (Henry and Cameron, 1983;Henry, 2001;Freire et al, 2008;Henry et al, 2012).…”

Section: Introductionmentioning

confidence: 96%

Analysis, characterisation and expression of gill-expressed carbonic anhydrase genes in the freshwater crayfish Cherax quadricarinatus

Ali

Pavasovic

Mather

et al. 2015

Gene

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Molecular cloning and expression of hepatopancreas glutamine synthetase in the Pacific white shrimp, Litopenaeus vannamei, induced by acute hypo-osmotic stress

Cited by 30 publications

References 49 publications

Transcriptional stress responses to environmental and husbandry stressors in aquaculture species

Transcriptional stress responses to environmental and husbandry stressors in aquaculture species

Evaluation of some haemolymph biochemical properties and F‐cell prevalence in hepatopancreas of white leg shrimp (Litopenaeus vanammei) after fed diets containing apple cider vinegar and propionic acid

Analysis, characterisation and expression of gill-expressed carbonic anhydrase genes in the freshwater crayfish Cherax quadricarinatus

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