Molecular cloning and expression pattern of 11 genes involved in lipid metabolism in yellow catfish Pelteobagrus fulvidraco

Zheng, Jia; Luo, Zhi; Zhu, Qing–Ling; Tan, Xiao-Ying; Chen, Qiliang; Sun, Luying; Hu, Wei

doi:10.1016/j.gene.2013.08.028

Cited by 102 publications

(70 citation statements)

References 43 publications

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“…Our recent study cloned the partial cDNA sequence of one single PPARc from yellow catfish (Zheng et al, 2013). However, Leaver et al (2005) reported the existence of multiple-alternative splice-variants in PPARc mRNAs of Atlantic salmon and European plaice.…”

Section: Discussionmentioning

confidence: 99%

“…The relative expression of genes was calculated with the ''delta-delta Ct'' method (Pfaffl, 2001). b-Actin was chosen as an internal reference for normalization for RT-PCR according to Zheng et al (2013).…”

Section: Mrna Quantification By Real-time Pcrmentioning

confidence: 99%

“…As a result of the rapid expansion of intensive aquaculture for yellow catfish, excess lipid deposition in the adipose tissue and liver of the fish species has seriously impacted growth performance and health. Recently, we cloned the partial cDNA sequence of PPARc and investigated mRNA tissue expression profiles of the single PPARc gene (Zheng et al, 2013). As a continuation of our studies involved in the structure and functions of the gene, the present study cloned the full-length cDNA sequences of two PPARc transcripts, and determined their tissue-specific and developmental expression profiles.…”

Section: Introductionmentioning

confidence: 95%

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Peroxisome proliferator-activated receptor gamma (PPARγ) in yellow catfish Pelteobagrus fulvidraco: Molecular characterization, mRNA expression and transcriptional regulation by insulin in vivo and in vitro

Zheng

Zhuo

Luo

et al. 2015

General and Comparative Endocrinology

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Section: Discussionmentioning

confidence: 99%

Section: Mrna Quantification By Real-time Pcrmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

See 1 more Smart Citation

Peroxisome proliferator-activated receptor gamma (PPARγ) in yellow catfish Pelteobagrus fulvidraco: Molecular characterization, mRNA expression and transcriptional regulation by insulin in vivo and in vitro

Zheng

Zhuo

Luo

et al. 2015

General and Comparative Endocrinology

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“…The existence of the PPAR subtypes α, β and γ has been demonstrated in fish (Mimeault et al, 2006;Zheng et al, 2013;Carmona-Antoñanzas et al, 2014), but to date it is unknown whether these nuclear receptors could be involved in the effects of OEA in these vertebrates. In addition, TRPV1 and GPR119 receptors have been shown to be involved in the feeding suppression actions of OEA in rodents (Ahern, 2003;Overton et al, 2006;Almási et al, 2008), although genetic removal of either TRPV1 or GPR119 has no effect on OEA-induced hypophagia (Piomelli, 2013).…”

Section: Research Articlementioning

confidence: 99%

Role of oleoylethanolamide as a feeding regulator in goldfish

Tinoco

Armirotti

Isorna

et al. 2014

Journal of Experimental Biology

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Oleoylethanolamide (OEA) is a bioactive lipid mediator, produced in the intestine and other tissues, which is involved in energy balance regulation in mammals, modulating feeding and lipid metabolism. The purpose of the present study was to investigate the presence and possible role of OEA in feeding regulation in goldfish (Carassius auratus). We assessed whether goldfish peripheral tissues and brain contain OEA and their regulation by nutritional status. OEA was detected in all studied tissues (liver, intestinal bulb, proximal intestine, muscle, hypothalamus, telencephalon and brainstem). Food deprivation (48 h) reduced intestinal OEA levels and levels increased upon re-feeding, suggesting that this compound may be involved in the short-term regulation of food intake in goldfish, as a satiety factor. Next, the effects of acute intraperitoneal administration of OEA on feeding, swimming and plasma levels of glucose and triglycerides were analysed. Food intake, swimming activity and circulating triglyceride levels were reduced by OEA 2 h post-injection. Finally, the possible interplay among OEA and other feeding regulators (leptin, cholecystokinin, ghrelin, neuropeptide Y, orexin and monoamines) was investigated. OEA actions on energy homeostasis in goldfish could be mediated, at least in part, through interactions with ghrelin and the serotonergic system, as OEA treatment reduced ghrelin expression in the intestinal bulb, and increased serotonergic activity in the telencephalon. In summary, our results indicate for the first time in fish that OEA could be involved in the regulation of feeding, swimming and lipid metabolism, suggesting a high conservation of OEA actions in energy balance throughout vertebrate evolution.

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“…Total RNA extraction and first strand cDNA synthesis were performed according to methods in Zheng et al (2013). The cDNA synthesis reactions were diluted to 200 μl in water.…”

Section: Mrna Level Determination By Quantitative Real-time Pcrmentioning

confidence: 99%

Effect and the related signaling pathways of insulin influencing lipid metabolism in yellow catfishPelteobagrus fulvidraco

Zhuo

Luo

Pan

et al. 2015

Journal of Experimental Biology

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The influence of insulin on hepatic metabolism in fish is not well understood. The present study was therefore conducted to investigate the effects of insulin on lipid metabolism, and the related signaling pathways, in the yellow catfish Pelteobagrus fulvidraco. Hepatic lipid and intracellular triglyceride (TG) content, the activity and expression levels of several enzymes and the mRNA expression of transcription factors (PPARα and PPARγ) involved in lipid metabolism were determined. Troglitazone, GW6471, fenofibrate and wortmannin were used to explore the signaling pathways by which insulin influences lipid metabolism. Insulin tended to increase hepatic lipid accumulation, the activity of lipogenic enzymes (6PGD, G6PD, ME, ICDH and FAS) and mRNA levels of FAS, G6PD, 6PGD, CPT IA and PPARγ, but down-regulated PPARα mRNA level. The insulin-induced effect could be stimulated by the specific PPARγ activator troglitazone or reversed by the PI3 kinase/Akt inhibitor wortmannin, demonstrating that signaling pathways of PPARγ and PI3 kinase/Akt were involved in the insulin-induced alteration of lipid metabolism. The specific PPARα pathway activator fenofibrate reduced insulininduced TG accumulation, down-regulated the mRNA levels of FAS, G6PD and 6PGD, and up-regulated mRNA levels of CPT IA, PPARα and PPARγ. The specific PPARα pathway inhibitor GW6471 reduced insulin-induced changes in the expression of all the tested genes, indicating that PPARα mediated the insulin-induced changes of lipid metabolism. The present results contribute new knowledge on the regulatory role of insulin in hepatic metabolism in fish.

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Molecular cloning and expression pattern of 11 genes involved in lipid metabolism in yellow catfish Pelteobagrus fulvidraco

Cited by 102 publications

References 43 publications

Peroxisome proliferator-activated receptor gamma (PPARγ) in yellow catfish Pelteobagrus fulvidraco: Molecular characterization, mRNA expression and transcriptional regulation by insulin in vivo and in vitro

Peroxisome proliferator-activated receptor gamma (PPARγ) in yellow catfish Pelteobagrus fulvidraco: Molecular characterization, mRNA expression and transcriptional regulation by insulin in vivo and in vitro

Role of oleoylethanolamide as a feeding regulator in goldfish

Effect and the related signaling pathways of insulin influencing lipid metabolism in yellow catfishPelteobagrus fulvidraco

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