Molecular cloning and expression profiles of nitric oxide synthase (NOS) in mud crab Scylla paramamosain

Li, Shengkang; Zhao, Zhigang; Li, Chuanbiao; Zhou, Lei; Liu, Wenhua; Li, Yuanyou; Zhang, Yueling; Zheng, Huaiping; Wen, Xiaobo

doi:10.1016/j.fsi.2011.12.002

Cited by 33 publications

(23 citation statements)

References 35 publications

(43 reference statements)

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“…While other crustacean NOSs have been described ( e.g. Inada et al, 2010; Kim et al, 2004; Labbé et al, 2009; Li et al, 2012; McCoole et al, 2012; McDonald et al, 2011; Rodríguez-Ramos et al, 2010; Yao et al, 2010), to the best of our knowledge, there is only one other report describing a crustacean HO (McCoole et al, 2012), and no studies describing crustacean CBS homologs have been published.…”

Section: Discussionmentioning

confidence: 97%

Diffusible gas transmitter signaling in the copepod crustacean Calanus finmarchicus: Identification of the biosynthetic enzymes of nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H2S) using a de novo assembled transcriptome

Christie¹,

Fontanilla²,

Roncalli³

et al. 2014

General and Comparative Endocrinology

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Neurochemical signaling is a major component of physiological/behavioral control throughout the animal kingdom. Gas transmitters are perhaps the most ancient class of molecules used by nervous systems for chemical communication. Three gases are generally recognized as being produced by neurons: nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H2S). As part of an ongoing effort to identify and characterize the neurochemical signaling systems of the copepod Calanus finmarchicus, the biomass dominant zooplankton in much of the North Atlantic Ocean, we have mined a de novo assembled transcriptome for sequences encoding the neuronal biosynthetic enzymes of these gases, i.e. nitric oxide synthase (NOS), heme oxygenase (HO) and cystathionine β-synthase (CBS), respectively. Using Drosophila proteins as queries, two NOS-, one HO-, and one CBS-encoding transcripts were identified. Reverse BLAST and structural analyses of the deduced proteins suggest that each is a true member of its respective enzyme family. RNA-Seq data collected from embryos, early nauplii, late nauplii, early copepodites, late copepodites and adults revealed the expression of each transcript to be stage specific: one NOS restricted primarily to the embryo and the other was absent in the embryo but expressed in all other stages, no CBS expression in the embryo, but present in all other stages, and HO expressed across all developmental stages. Given the importance of gas transmitters in the regulatory control of a number of physiological processes, these data open opportunities for investigating the roles these proteins play under different life-stage and environmental conditions in this ecologically important species.

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Section: Discussionmentioning

confidence: 97%

Diffusible gas transmitter signaling in the copepod crustacean Calanus finmarchicus: Identification of the biosynthetic enzymes of nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H2S) using a de novo assembled transcriptome

Christie¹,

Fontanilla²,

Roncalli³

et al. 2014

General and Comparative Endocrinology

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“…Thus, compared to vertebrate enzymes, the MnNOS sequence exhibited a relatively high level of identity with crustacean NOSs (Figure 3). This evolutionary relationship implies that, with the exception of iNOS produced by the arthropod Limulus polyphemus, different isoforms of this enzyme may not be found in invertebrates, and this lineage may have inherited only on eNOS type, as in mollusks (Andreakis et al, 2011), the mud crab Scylla paramamosain (Li et al, 2012), and the Zhikong scallop Chlamys farreri (Jiang et al, 2013). Furthermore, the observed connections between these groups might reflect taxonomic relationships, and appear to show that although MnNOS clustered with invertebrate NOS enzymes, it was not closely related to that of any species examined.…”

Section: Discussionmentioning

confidence: 99%

“…And McDonald et al (2011) found high expression of NOS in Y-organs of the green shore crab Carcinus maenas. In the Pacific white shrimp L. vannamei and the mud crab S. paramamosain, expression has been detected in the hepatopancreas (Yao et al, 2010;Li et al, 2012), whereas in the kuruma shrimp M. japonicus, it has been observed in the gills and brain (Inada et al, 2010). In addition, iNOS is expressed in hemocytes of the spiny lobster Panulirus argus (Rodríguez-Ramos et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning and expression pattern of oriental river prawn (Macrobrachium nipponense) nitric oxide synthase

Rahman

Sun

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Nitric oxide synthase (NOS) produces nitric oxide (NO) by catalyzing the conversion of l-arginine to l-citrulline, with the concomitant oxidation of nicotinamide adenine dinucleotide phosphate. Recently, various studies have verified the importance of NOS invertebrates and invertebrates. However, the NOS gene family in the oriental river prawn Macrobrachium nipponense is poorly understood. In this study, we cloned the full-length NOS complementary DNA from M. nipponense (MnNOS) and characterized its expression pattern in different tissues and at different developmental stages. Real-time quantitative polymerase chain reaction (RT-qPCR) showed the MnNOS gene to be expressed in all investigated tissues, with the highest levels observed in the androgenic gland (P < 0.05). Our results revealed that the MnNOS gene may play a key role in M. nipponense male sexual differentiation. Moreover, RT-qPCR revealed that MnNOS mRNA expression was significantly increased in post-larvae 10 days after metamorphosis (P < 0.05). The expression of this gene in various tissues indicates that it may perform versatile biological functions in M. nipponense.

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“…Decapod crustacean tissues express a single NOS that resembles the Ca Lee et al, 2000;Scholz et al, 2002;Zou et al, 2002;Kim et al, 2004;McDonald et al, 2011). The crustacean NOS gene is expressed in many tissues, which is consistent with its role as a regulator of diverse physiological functions (Lee et al, 2000;Kim et al, 2004;Inada et al, 2010;Yao et al, 2010;McDonald et al, 2011;Li et al, 2012;Wu et al, 2013).…”

Section: Introductionmentioning

confidence: 88%

Nitric oxide production and sequestration in the sinus gland of the green shore crab, Carcinus maneas

Pitts

Mykles

2014

Journal of Experimental Biology

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Molting in decapod crustaceans is regulated by molt-inhibiting hormone (MIH), a neuropeptide produced in the X-organ (XO)/sinus gland (SG) complex of the eyestalk ganglia (ESG). Pulsatile release of MIH from the SG suppresses ecdysteroidogenesis by the molting gland or Y-organ (YO). The hypothesis is that nitric oxide (NO), a neuromodulator that controls neurotransmitter release at presynaptic membranes, depresses the frequency and/or amount of MIH pulses to induce molting. NO synthase (NOS) mRNA was present in Carcinus maenas ESG and other tissues and NOS protein was present in the SG. A copper based ligand (CuFL), which reacts with NO to form a highly fluorescent product (NO-FL), was used to image NO in the ESG and SG and quantify the effects of NO scavenger (cPTIO), NOS inhibitor (L-NAME), and sodium azide (NaN 3 ) on NO production in the SG. Pre-incubation with cPTIO prior to CuFL loading decreased NO-FL fluorescence ~30%; including L-NAME had no additional effect. Incubating SG with L-NAME during preincubation and loading decreased NO-FL fluorescence ~40%, indicating that over half of the NO release was not directly dependent on NOS activity. Azide, which reacts with NO-binding metal groups in proteins, reduced NO-FL fluorescence to near background levels without extensive cell death. Spectral shift analysis showed that azide displaced NO from a soluble protein in SG extract. These data suggest that the SG contains NO-binding protein(s) that sequester NO and releases it over a prolonged period. This NO release may modulate neuropeptide secretion from the axon termini in the SG.

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Molecular cloning and expression profiles of nitric oxide synthase (NOS) in mud crab Scylla paramamosain

Cited by 33 publications

References 35 publications

Diffusible gas transmitter signaling in the copepod crustacean Calanus finmarchicus: Identification of the biosynthetic enzymes of nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H2S) using a de novo assembled transcriptome

Diffusible gas transmitter signaling in the copepod crustacean Calanus finmarchicus: Identification of the biosynthetic enzymes of nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H2S) using a de novo assembled transcriptome

Molecular cloning and expression pattern of oriental river prawn (Macrobrachium nipponense) nitric oxide synthase

Nitric oxide production and sequestration in the sinus gland of the green shore crab, Carcinus maneas

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