Molecular Cloning and Function Characterization in Feeding of Neuropeptide Y in Gibel Carp, Carassius auratus gibelio

Luo, Lei; Deng, Xingxing; Yuan, Dengyue; Zheng, Zonglin; Zhu, Changlian; Luo, Hongyu; Li, Baohai; Ye, Hua; Zhou, Chaowei

doi:10.17582/journal.pjz/2019.51.3.1017.1025

Cited by 3 publications

(1 citation statement)

References 19 publications

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“…The expression of Cyp19a1b is under the regulation of estrogen, and through this feedback loop, the gonads gradually differentiate into ovaries (Brion et al 2012). A comparative analysis of Cyp19a1b distribution and activity in the brain, as well as estrogen receptor expression, indicates that this gene reaches its peak expression during the neuroembryonic stage of embryos (Li, 2019). Additionally, the preoptic area and hypothalamus are identi ed as the primary targets of estrogen synthesis in the brain, suggesting a connection between endogenous estrogen production in the brain and neuroendocrine function (Hutchison et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Expression characteristics of the cyp19a1b aromatase gene and its response to 17β-estradiol treatment in largemouth bass (Micropterus salmoides)

Zhang,

Tian,

Han

et al. 2024

Fish Physiol Biochem

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To investigate the regulatory role of the Cyp19a1b aromatase gene in the sexual differentiation of largemouth bass (Micropterus salmoides, LMB), we obtained the full-length cDNA sequence of Cyp19a1b using rapid ampli cation of cDNA ends technique. Tissue expression characteristics and feedback with 17-β-estradiol (E 2 ) were determined using quantitative real-time PCR (qRT-PCR), while gonad development was assessed through histological section observations. The cDNA sequence of LMB Cyp19a1b was found to be1950 base pairs (bp) in length, including a 5' untranslated region of 145 bp, a 3' untranslated region of 278 bp, and an open reading frame encoding a protein consisting of 1527 bp that encoded 508 amino acids. The qRT-PCR results indicated that Cyp19a1b abundantly expressed in the brain, followed by the gonads, and its expression in the ovaries was signi cantly higher than that observed in the testes.(P < 0.05). After feeding sh with E 2 for 30 days, the expression of Cyp19a1b in the pseudo-females gonads (XY-F) was signi cantly higher than that in males (XY-M) (P < 0.05), whereas expression did not differ signi cantly between female (XX-F) and XY-M sh (P > 0.05). Although the expression of Cyp19a1b in XY-F and XX-F sh was not signi cantly different after 60 days (P > 0.05), both exhibited signi cantly higher levels than that of XY-M sh (P < 0.05). Histological sections analysis showed the presence of oogonia in both XY-F and XX-F sh at 30 days, while spermatogonia were observed in XY-M sh. At 60 days, primary oocytes were abundantly observed in both XY-F and XX-F sh, while a few spermatogonia were visible in XY-M sh. At 90 days, the histological sections results showed that a large number of oocytes were visible in XY-F and XX-F sh. Additionally, the gonads of XY-M sh contained numerous spermatocytes. These results suggest that Cyp19a1b plays a pivotal role in the development of ovaries and nervous system development in LMB.

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Section: Introductionmentioning

confidence: 99%

Expression characteristics of the cyp19a1b aromatase gene and its response to 17β-estradiol treatment in largemouth bass (Micropterus salmoides)

Zhang,

Tian,

Han

et al. 2024

Fish Physiol Biochem

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show abstract

Expression characteristics of the Cyp19a1b aromatase gene and its response to 17β-estradiol treatment in largemouth bass (Micropterus salmoides)

Zhang,

Tian,

Han

et al. 2023

Preprint

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To investigate the regulatory role of the Cyp19a1b aromatase gene in the sexual differentiation of largemouth bass (Micropterus salmoides, LMB), we obtained the full-length cDNA sequence of Cyp19a1b using rapid amplification of cDNA ends technique. Tissue expression characteristics and feedback with 17-β-estradiol (E2) were determined using quantitative real-time PCR (qRT-PCR), while gonad development was assessed through histological section observations. The cDNA sequence of LMB Cyp19a1b was found to be1950 base pairs (bp) in length, including a 5' untranslated region of 145 bp, a 3' untranslated region of 278 bp, and an open reading frame encoding a protein consisting of 1527 bp that encoded 508 amino acids. The qRT-PCR results indicated that Cyp19a1b abundantly expressed in the brain, followed by the gonads, and its expression in the ovaries was significantly higher than that observed in the testes. (P < 0.05). After feeding fish with E2 for 30 days, the expression of Cyp19a1b in the pseudo-females gonads (XY-F) was significantly higher than that in males (XY-M) (P < 0.05), whereas expression did not differ significantly between female (XX-F) and XY-M fish (P > 0.05). Although the expression of Cyp19a1b in XY-F and XX-F fish was not significantly different after 60 days (P > 0.05), both exhibited significantly higher levels than that of XY-M fish (P < 0.05). Histological sections analysis showed the presence of oogonia in both XY-F and XX-F fish at 30 days, while spermatogonia were observed in XY-M fish. At 60 days, primary oocytes were abundantly observed in both XY-F and XX-F fish, while a few spermatogonia were visible in XY-M fish. At 90 days, the histological sections results showed that a large number of oocytes were visible in XY-F and XX-F fish. Additionally, the gonads of XY-M fish contained numerous spermatocytes. These results suggest that Cyp19a1b plays a pivotal role in the development of ovaries and nervous system development in LMB.

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Neuropeptide Y in Spotted Scat (Scatophagus Argus), Characterization and Functional Analysis towards Feed Intake Regulation

Assan

Wang²,

Mustapha

et al. 2022

Fishes

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Neuropeptide Y (Npy) is an intricate neuropeptide regulating numerous physiological processes. It is a highly conserved peptide known to improve feed intake in many vertebrates, including fishes. To enlighten the mechanism of Npy in spotted scat feed intake control, we cloned and identified the Npy cDNA sequence. We further examined its expression in some tissues and explored its expression effects at different time frames (hours and days). Here, we discovered that spotted scat Npy comprised a 300 bp open reading frame (ORF) and a 99 amino acid sequence. Npy was identified to be expressed in all tissues examined. Using in situ hybridization examination, we proved that npy has a wide expression in the brain of the spotted scat. Furthermore, the expression of npy in the hypothalamus significantly increased one hour after feeding (p < 0.05). Further, it was revealed that npy expression significantly increased in fish that were fasted for up to 5 days and significantly increased after refeeding from the 8th to the 10th day. This suggests that Npy is an orexigenic peptide, and hence, it increases food intake and growth in the spotted scat. Additionally, results from in vitro and in vivo experiments revealed that Npy locally interacts with other appetite-regulating peptides in the spotted scat hypothalamus. This research aimed to set a fundamental study in developing the feed intake regulation, improving growth and reproduction, which is significant to the aquaculture industry of the spotted scat.

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Molecular Cloning and Function Characterization in Feeding of Neuropeptide Y in Gibel Carp, Carassius auratus gibelio

Cited by 3 publications

References 19 publications

Expression characteristics of the cyp19a1b aromatase gene and its response to 17β-estradiol treatment in largemouth bass (Micropterus salmoides)

Expression characteristics of the cyp19a1b aromatase gene and its response to 17β-estradiol treatment in largemouth bass (Micropterus salmoides)

Expression characteristics of the Cyp19a1b aromatase gene and its response to 17β-estradiol treatment in largemouth bass (Micropterus salmoides)

Neuropeptide Y in Spotted Scat (Scatophagus Argus), Characterization and Functional Analysis towards Feed Intake Regulation

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