Molecular cloning and functional analysis of two FAD2 genes from American grape (Vitis labrusca L.)

Lee, Kyeong‐Ryeol; Kim, Sun Hee; Go, Young-Sam; Jung, Sung Min; Roh, Kyung Hee; Kim, Jong Bum; Suh, Mi-Chung; Lee, Sukchan; Kim, Hyun Uk

doi:10.1016/j.gene.2012.08.032

Cited by 31 publications

(23 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3), which was consistent with its tissue expression profile (Fig. 4), whereas AsFAD2-1 was strongly expressed in developing seeds, which was similar to the expression patterns of FAD2-1 genes in most plants such as cotton and grape [ 37,49]. The phylogenetic analysis also revealed that the AsFAD2-10 gene was constitutively expressed (Fig.…”

Section: Discussionsupporting

confidence: 77%

Cloning and Functional Analysis of the FAD2 Gene Family from Desert Shrub Artemisia sphaerocephala

Miao

zhang

et al. 2019

Preprint

View full text Add to dashboard Cite

Background: Linoleic acid is an important polyunsaturated fatty acid, required for all eukaryotes. Microsomal delta-12 (Δ12) oleate desaturase (FAD2) is a key enzyme for linoleic acid biosynthesis. Desert shrub Artemisia sphaerocephala is rich in linoleic acid, it has a large FAD2 gene family with twenty-six members. The objective of this work is to study the difference and potentially functionality of AsFAD2 family members. Results: Full-length cDNAs of twenty-one AsFAD2 genes were obtained from A. sphaerocephala. The putative polypeptides encoded by AsFAD2 family genes showed a high level of sequence similarity and were relatively conserved during evolution. The motif composition was relatively conservative. Quantitative real-time PCR analysis revealed that the AsFAD2-1 gene was strongly expressed in developing seeds, which may be closely associated with the high accumulating ability of linoleic acid in A. sphaerocephala seeds. Although different AsFAD2 family members showed diverse response to salt stress, the overall mRNA levels of the AsFAD2 family genes was stable, suggesting that the AsFAD2 family may play an important role in the adaptation of A. sphaerocephala to the harsh desert environment. Transient expression of AsFAD2 genes in the Nicotiana benthamiana leaves revealed that the encoded proteins were all located in the endoplasmic reticulum. Heterologous expression in Saccharomyces cerevisiae suggested that only three AsFAD2 enzymes, AsFAD2-1, -10, and -23, were Δ12 oleate desaturases, which could convert oleic acid to linoleic acid, whereas AsFAD2-1 and AsFAD2-10 could also produce palmitolinoleic acid. Conclusions: This research reported the cloning, expression studies, subcellular localization and functional identification of the large AsFAD2 gene family. These results should be helpful in understanding of fatty acid biosynthesis in A. sphaerocephala, and can be applied in the study of plant fatty acids traits.

show abstract

Section: Discussionsupporting

confidence: 77%

Cloning and Functional Analysis of the FAD2 Gene Family from Desert Shrub Artemisia sphaerocephala

Miao

zhang

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…Lee et al (2012) reported that -6 and -3 fatty acid desaturase (FAD) played a key role in the synthesis of the unsaturated fatty acids linoleic (18:2) and linolenic (18:3) acids. Expression of the yeast -9 desaturase gene (Wang et al, 1996) led to changes in the levels of fatty acids (saturated and unsaturated) in tomato fruit (Lycopersicon esculentum Mill.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome profiling reveals the candidate genes associated with aroma metabolites and emission of pear (Pyrus ussuriensis cv.)

Wei

Tao

Qin

et al. 2016

Scientia Horticulturae

View full text Add to dashboard Cite

Transcriptome profiling reveals the candidate genes associated with aroma metabolites and emission of pear (Pyrus ussuriensis cv.) ଝ a b s t r a c t Aroma is an important factor affecting pear fruit quality. However, the molecular basis for the development of aroma metabolites and their emission is poorly understood. Unripe 'Nanguoli' pear fruit has poor aroma, while the ripe fruit has an intense aroma, and calcium treatment can enhance the aroma, providing good material for transcriptome analysis.Digital gene expression (DGE) analysis of 'Nanguoli' fruit was performed at three important developmental stages, with and without calcium treatment, six libraries in total. Of the total number of mapped reads (11.4-13.6 million), an average of 69.81% could be mapped to the pear genome. A total of 10,776 differentially expressed genes between the poor aroma and intense aroma were categorized into 36 functional groups and 37 pathways according to GO and KEGG databases, respectively. Genes more highly expressed in the more aromatic fruit include some from the fatty acid pathway such as FAD, ADH, PDC, LOX, and lipase genes. Some transcription factors were identified with high correlation to aroma biosynthesis in pear. Also, ABA may play an important role in the calcium-triggered improvement of 'Nanguoli' aroma. In addition, candidate genes were verified with quantitative real-time PCR.

show abstract

“…The number of transmembrane domains among plant FAD2 enzymes is different, it is usually in the range of three to six. Thus, red flax (Linum grandiflorum), pumpkin (Cucurbita pepo), sesame (S. indicum), and grape (Vitis labrusca) enzymes contain 3, 4, 5, and 6 transmembrane regions, respectively [33,34]. Our data indicated that the AsFAD2 family members were structurally diverse.…”

Section: Discussionmentioning

confidence: 95%

Cloning and Functional Analysis of the FAD2 Gene Family from Desert Shrub Artemisia sphaerocephala

Miao

zhang

et al. 2019

Preprint

View full text Add to dashboard Cite

Background: Linoleic acid is an important polyunsaturated fatty acid, required for all eukaryotes.Microsomal delta-12 (Δ12) oleate desaturase (FAD2) is a key enzyme for linoleic acid biosynthesis.Desert shrub Artemisia sphaerocephala is rich in linoleic acid, it has a large FAD2 gene family with twenty-six members. The aim of this work is to unveil the difference and potentially functionality of AsFAD2 family members. Results: Full-length cDNAs of twenty-one AsFAD2 genes were obtained from A. sphaerocephala. The putative polypeptides encoded by AsFAD2 family genes showed a high level of sequence similarity and were relatively conserved during evolution. The motif composition was also relatively conservative. Quantitative real-time PCR analysis revealed that the AsFAD2-1 gene was strongly expressed in developing seeds, which may be closely associated with the high accumulating ability of linoleic acid in A. sphaerocephala seeds. Although different AsFAD2 family members showed diverse response to salt stress, the overall mRNA levels of the AsFAD2 family genes was stable.Transient expression of AsFAD2 genes in the Nicotiana benthamiana leaves revealed that the encoded proteins were all located in the endoplasmic reticulum. Heterologous expression in Saccharomyces cerevisiae suggested that only three AsFAD2 enzymes, AsFAD2-1, -10, and -23, were Δ12 oleate desaturases, which could convert oleic acid to linoleic acid, whereas AsFAD2-1 and AsFAD2-10 could also produce palmitolinoleic acid. Conclusions: This research reported the cloning, expression studies, subcellular localization and functional identification of the large AsFAD2 gene family. These results should be helpful in understanding fatty acid biosynthesis in A. sphaerocephala, and has the potential to be applied in the study of plant fatty acids traits.

show abstract

Molecular cloning and functional analysis of two FAD2 genes from American grape (Vitis labrusca L.)

Cited by 31 publications

References 23 publications

Cloning and Functional Analysis of the FAD2 Gene Family from Desert Shrub Artemisia sphaerocephala

Cloning and Functional Analysis of the FAD2 Gene Family from Desert Shrub Artemisia sphaerocephala

Transcriptome profiling reveals the candidate genes associated with aroma metabolites and emission of pear (Pyrus ussuriensis cv.)

Cloning and Functional Analysis of the FAD2 Gene Family from Desert Shrub Artemisia sphaerocephala

Contact Info

Product

Resources

About