Molecular Cloning and Functional Characterization of Xenopus tropicalis Frog Transient Receptor Potential Vanilloid 1 Reveal Its Functional Evolution for Heat, Acid, and Capsaicin Sensitivities in Terrestrial Vertebrates

Ohkita, Masaaki; Saito, Shigeru; Imagawa, Toshiaki; Takahashi, Kenji; Tominaga, Makoto; Ohta, Toshio

doi:10.1074/jbc.m111.305698

Cited by 42 publications

(58 citation statements)

References 41 publications

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“…It is suggested that TRPV1 sensitivity to capsaicin is an evolutionary acquisition in mammals, as zebrafi sh, amphibian and avian TRPV1 are residually sensitive to this substance (Jordt & Julius, 2002;Ohkita et al, 2012;Gau et al, 2013). Although insect vanilloid receptors (Nanchung and Inactive) do not respond to capsaicin, this alkaloid is reported to repel some species of insects, for example, Sitophilus zeamais (Spurr & McGregor, 2003).…”

Section: Discussionmentioning

confidence: 99%

Capsazepine affects thermal preferences of the American cockroach (Blattodea: Blattidae)

Maliszewska¹,

Tęgowska²

2016

Eur. J. Entomol.

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ISSN (online): 1802-8829http://www.eje.cz capsaicin induces preferences for lower temperatures in Rhodnius prolixus, while capsazepine treatment cause the opposite behaviour.The aim of our study was to assess the effect of capsazepine (TRPV1 antagonist) on the thermal preference of the American cockroach, Periplaneta americana. American cockroach has TRP receptors. Wicher et al. (2005) screened the Periplaneta cDNA library for members of the TRPC family and found a homolog of the Drosophila TRPɣ channel. It is proposed that it is involved in forming the channel that conducts the Ca 2+ background current in DUM neurons. Recently, French et al. (2015) demonstrated that the TRPL channel has a major role in Periplaneta phototransduction.Based on our preliminary results we hypothesized that capsazepine infl uences thermoregulatory processes in insects. To confi rm this hypothesis, we examined thermal preferences of cockroaches treated with fi ve different capsazepine concentrations and released in a thermal gradient system. To establish whether capsazepine may act as a capsaicin antagonist in insects, we assessed the insect thermoregulatory response to capsaicin following pretreatment with capsazepine. We assumed that capsazepine blocks the insects' hypothermic response to capsaicin, as in mammals (Dogan et al., 2004). MATERIALS AND METHODS InsectsAmerican cockroaches Periplaneta americana L. were reared in plastic containers at ~ 26°C under a natural photoperiod. They were fed with oat fl akes and apples and received drinking water at regular intervals. Only adult individuals were selected for the experiments. Experiments were performed separately on males Abstract. Capsazepine is a competitive antagonist of capsaicin, a TRPV1 agonist responsible for the spicy taste of pepper. TRPV1 agonists and antagonists are known to affect mammalian body temperature, but their action on thermoregulation in insects is poorly known. In this study we evaluated the effect of capsazepine on the thermal preference of the American cockroach, Periplaneta americana using a thermal gradient. Our results revealed that capsazepine in submicromolar concentrations induces a preference for higher ambient temperatures when compared to the control insects. To assess whether capsazepine may act also as an antagonist of capsaicin in insects, we determined this insects' thermal behaviour when capsazepine was applied before capsaicin. The hypothermic response to capsaicin was clearly blocked by pre-treatment with capsazepine only in female American cockroaches. Our results indicate the involvement of structures functionally similar to TRPV1 in insect thermosensation.

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Section: Discussionmentioning

confidence: 99%

Capsazepine affects thermal preferences of the American cockroach (Blattodea: Blattidae)

Maliszewska¹,

Tęgowska²

2016

Eur. J. Entomol.

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“…For example, capsaicin, a transient receptor potential vanilloid 1 (TRPV1) agonist, activates human and rodent TRPV1 (22,23). However, rabbit, western clawed frog, and chicken TRPV1s exhibit lower sensitivity to capsaicin (23)(24)(25). Regarding TRPA1, menthol activates mouse TRPA1 at low concentrations but blocks it at high concentrations, whereas it only activates human TRPA1 (26).…”

Section: Trpa1mentioning

confidence: 99%

Molecular Basis Determining Inhibition/Activation of Nociceptive Receptor TRPA1 Protein

Banzawa

Saito

Imagawa

et al. 2014

Journal of Biological Chemistry

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Background: Nociceptive TRPA1 is an important drug discovery target for identification of analgesics. Results: A967079, one of the most potent mammalian TRPA1 antagonists, showed marked species differences. Conclusion: A single amino acid was responsible for inhibitory/stimulatory actions of A967079. Significance: The present data provide novel insight in the search for analgesics targeting TRPA1.

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“…TRPV1 was identified in frogs and its involvement was investigated in pain perception after a heat noxious stimulation (Ohkita et al, 2012). Noxious heat was applied to frog Dorsal Root Ganglion (DRG) neurons and consequently they produced a membrane current with similar properties to mammalian primary sensory neurons.…”

Section: Frogsmentioning

confidence: 99%

Transient Receptor Potential Vanilloid 1 Involvement in Animal Pain Perception

Vercelli¹,

Barbero²

2015

American Journal of Animal and Veterinary Sciences

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In last decades, Transient Receptor Potential Vanilloid 1 (TRPV1) has been the target of a large number of scientific investigations. It has been identified as a polymodal transducer molecule on a sub-set of primary sensory neurons which responds to various endogenous and exogenous stimuli including noxious heat (more than 42°C), protons and vanilloids such as capsaicin, the hot ingredient of chilli peppers. In mammals, TRPV1 displays a wide tissue and cellular expression including both the peripheral and central nervous system, with broad distribution and functions, in physiological and pathological conditions. Its primary localisation in sensory neurons reveals its key nodal point in pain transmission pathways. Nowadays, it is clear that TRPV1 is involved in inflammation, pain perception and thermoregulation in the majority of animals, including humans. Recently, a lot of studies tried to investigate some analgesic treatments applied on TRPV1. The aim of this review is to give to the readers a short overview of the TRPV1 involvement in pain perception and possible therapeutic applications, highlighting this topic in species of interest in veterinary medicine.

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Molecular Cloning and Functional Characterization of Xenopus tropicalis Frog Transient Receptor Potential Vanilloid 1 Reveal Its Functional Evolution for Heat, Acid, and Capsaicin Sensitivities in Terrestrial Vertebrates

Cited by 42 publications

References 41 publications

Capsazepine affects thermal preferences of the American cockroach (Blattodea: Blattidae)

Capsazepine affects thermal preferences of the American cockroach (Blattodea: Blattidae)

Molecular Basis Determining Inhibition/Activation of Nociceptive Receptor TRPA1 Protein

Transient Receptor Potential Vanilloid 1 Involvement in Animal Pain Perception

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