Molecular cloning and nucleotide sequence analysis of genes from a cDNA library of the scorpion Tityus discrepans

D’Suze, Gina; Schwartz, Elisabeth Ferroni; García‐Gómez, Blanca I.; Sevcik, Carlos; Possani, Lourival D.

doi:10.1016/j.biochi.2009.05.005

Cited by 57 publications

(43 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…al., 2010; reviewed in Rodríguez de la Vega et al, 2013). The results of cDNA analysis also show that the venomous glands of scorpions have many components related to cellular processes, protein synthesis, protein trafficking and others (Schwartz et al, 2007; Kozminsky-Atias et al, 2008; Silva et al, 2009; D’Suze et al, 2009; Ma et al, 2009; Ruiming et al, 2010; Ma et al, 2010; Morgenstern et al, 2011). …”

Section: Na+-channel Specific Toxinsmentioning

confidence: 89%

“…Recently, the identification of venom components have been obtained by sequencing cDNA clones of cDNA libraries of venomous glands obtained from scorpions of different families (Schwartz et al, 2007; Kozminsky-Atias et al, 2008; D’Suze et al, 2009; Ma et al, 2009; Roeding et al, 2009; Silva et al, 2009; Ma et al, 2010; Ruiming et al, 2010; Morgenstern et al, 2011; Almeida 2012; Diego-García 2012; Ma et al, 2012; Rendón-Anaya 2012; Luna-Ramírez et al, 2013). …”

Section: Na+-channel Specific Toxinsmentioning

confidence: 99%

See 1 more Smart Citation

Scorpion venom components that affect ion-channels function

Quintero-Hernández

Jiménez-Vargas

Gurrola

et al. 2013

Toxicon

234

168

View full text Add to dashboard Cite

SUMMARY The number and types of venom components that affect ion-channel function are reviewed. These are the most important venom components responsible for human intoxication, deserving medical attention, often requiring the use of specific anti-venoms. Special emphasis is given to peptides that recognize Na+-, K+- and Ca++-channels of excitable cells. Knowledge generated by direct isolation of peptides from venom and components deduced from cloned genes, whose amino acid sequences are deposited into databanks are now adays in the order of 1.5 thousands, out of an estimate biodiversity closed to 300,000. Here the diversity of components is briefly reviewed with mention to specific references. Structural characteristic are discussed with examples taken from published work. The principal mechanisms of action of the three different types of peptides are also reviewed. Na+-channel specific venom components usually are modifier of the open and closing kinetic mechanisms of the ion-channels, whereas peptides affecting K+-channels are normally pore blocking agents. The Ryanodine Ca++-channel specific peptides are known for causing sub-conducting stages of the channels conductance and some were shown to be able to internalize penetrating inside the muscle cells.

show abstract

Section: Na+-channel Specific Toxinsmentioning

confidence: 89%

Section: Na+-channel Specific Toxinsmentioning

confidence: 99%

Scorpion venom components that affect ion-channels function

Quintero-Hernández

Jiménez-Vargas

Gurrola

et al. 2013

Toxicon

234

168

View full text Add to dashboard Cite

show abstract

“…These toxins may act by two possible mechanisms: they are able to inhibit the angiotensin converting enzymes (ACEs) or able to sensitize the bradykinin receptor (Meki et al, 1995). Few sequences similar to BPPs have been reported in members of Tityus genus, in T. discrepans (D'Suze et al, 2009) and in T. serrulatus (Rates et al, 2008;Alvarenga et al, 2012).…”

Section: Other Putative Venom Componentsmentioning

confidence: 97%

“…Butantoxin, on the other hand, is a potassium channel blocker purified from T. serrulatus, T. bahiensis and T. stigmurus (Holaday et al, 2000). Peptides and enzymatic proteins present in the composition of the venom from some scorpion species have been investigated by different approaches such as transcriptomic and proteomic methods (Kalapothakis et al, 2001;Pimenta et al, 2001;Goudet et al, 2002;Schwartz et al, 2007;Ma et al, 2009Ma et al, , 2010Ruiming et al, 2010;Morgenstern et al, 2011;D'Suze et al, 2009;Batista et al, 2006Batista et al, , 2007Bringans et al, 2008;Rates et al, 2008;Ma et al, 2012;Diego-Garcia et al, 2012;Almeida et al, 2012;Alvarenga et al, 2012;Rend on-Anaya et al, 2012;Abdel-Rahman et al, 2013;He et al, 2013;Luna-Ramírez et al, 2013;Valdez-Vel azquez et al, 2013). However, no large-scale effort has been carried out for T. bahiensis and only a few reports of sequencing specific toxins are found in the literature (Becerril et al, 1996(Becerril et al, , 1997Trequattrini et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

The transcriptome recipe for the venom cocktail of Tityus bahiensis scorpion

Oliveira

Cândido

Dorce

et al. 2015

Toxicon

View full text Add to dashboard Cite

Scorpion venom is a mixture of peptides, including antimicrobial, bradykinin-potentiating and anionic peptides and small to medium proteins, such as ion channel toxins, metalloproteinases and phospholipases that together cause severe clinical manifestation. Tityus bahiensis is the second most medically important scorpion species in Brazil and it is widely distributed in the country with the exception of the North Region. Here we sequenced and analyzed the transcripts from the venom glands of T. bahiensis, aiming at identifying and annotating venom gland expressed genes. A total of 116,027 long reads were generated by pyrosequencing and assembled in 2891 isotigs. An annotation process identified transcripts by similarity to known toxins, revealing that putative venom components represent 7.4% of gene expression. The major toxins identified are potassium and sodium channel toxins, whereas metalloproteinases showed an unexpected high abundance. Phylogenetic analysis of deduced metalloproteinases from T. bahiensis and other scorpions revealed a pattern of ancient and intraspecific gene expansions. Other venom molecules identified include antimicrobial, anionic and bradykinin-potentiating peptides, besides several putative new venom components. This report provides the first attempt to massively identify the venom components of this species and constitutes one of the few transcriptomic efforts on the genus Tityus.

show abstract

“…Homologs of T. serrulatus Ts1 had only been found so far in other Brazilian Tityus such as T. bahiensis, T. stigmurus and T. costatus, species belonging to the subgenus Tityus (32,35). Significantly, T. discrepans and T. zulianus, two species sympatric with T. clathratus in Venezuela, do not produce such homologs but Na v toxins with divergent N-and C-termini with respect to Ts1 (10,33 Thus far T. clathratus is the only species from the north of the Amazon Basin producing putative toxin homologs of southern Brazilian Tityus, which provides further support for the evolutionary relationship between subgenera Archaeotityus (which has a trans-Amazonian distribution) and Tityus and its separation from the subgenus Atreus as suggested previously by Borges et al (4). The latter authors, in their molecular phylogenetic analysis of 21 Tityus species using two mitochondrial DNA markers (cytochrome oxidase subunit 1 and ribosomal 16S rRNA), found that the Archaeotityus type species, T. clathratus, groups with the Brazilian T. serrulatus into a single clade which strongly diverges (53-57% nucleotide divergence) with respect to its other congeners in Venezuela, Trinidad and Panama in the subgenus Atreus.…”

mentioning

confidence: 99%

Scorpions from the primeval subgenus Archaeotityus produce putative homologs of Tityus serrulatus toxins active on voltage-gated sodium channels

Borges¹,

Jowers²,

Bónoli³

et al. 2012

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

It has been proposed that the subgenus Archaeotityus comprises the most ancient species group within the medically important scorpion genus Tityus. cDNA encoding sodium-channel active toxins from the type species of this subgenus, Tityus clathratus (central Venezuela), have been isolated and sequenced. Two cDNAs were retrieved that encoded 61 amino acid-long putative neurotoxins named Tcl1 and Tcl2. Sequence identity was highest (87%) when both were compared with β-toxin Ts1 from the Brazilian scorpion Tityus serrulatus and its homologs from T. bahiensis, T. stigmurus, and T. costatus. A Bayesian analysis indicated statistical support for the grouping of T. clathratus Tcl1 and Tcl2 with Brazilian gammalike β-toxins, reinforcing previous phylogenetic studies which suggested an evolutionary relationship between the subgenus Archaeotityus and scorpion species inhabiting southeast South America belonging to the subgenus Tityus.

show abstract

Molecular cloning and nucleotide sequence analysis of genes from a cDNA library of the scorpion Tityus discrepans

Cited by 57 publications

References 74 publications

Scorpion venom components that affect ion-channels function

Scorpion venom components that affect ion-channels function

The transcriptome recipe for the venom cocktail of Tityus bahiensis scorpion

Scorpions from the primeval subgenus Archaeotityus produce putative homologs of Tityus serrulatus toxins active on voltage-gated sodium channels

Contact Info

Product

Resources

About