Molecular cloning and nucleotide sequence of the pestivirus bovine viral diarrhea virus

Collett, Marc S.; Larson, Ruby J.; Gold, Conrad; Strick, D. M.; Anderson, Dennis K.; Purchio, Anthony F.

doi:10.1016/0042-6822(88)90672-1

Cited by 448 publications

(248 citation statements)

References 19 publications

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“…The generated product was sequenced using the BigDye sequencing kit (BigDye Terminator v3.1 Cycle Sequencing Kit, Applied Biosystems, Warrington, UK) according to the instructions of the manufacturer, using the same primers. A 183-nt fragment of the 5' NCR, corresponding to position 160-341 of BVDV NADL [2], was used for comparative sequence analyses. The nucleotide sequences were assembled and proofread using the SeqMan II and EditSeq programmes in the DNASTAR programme package (DNASTAR Inc., Madison, WI, USA), then aligned and compared by the Clustal W method of the MegAlign programme from the same package.…”

Section: Molecular Methodsmentioning

confidence: 99%

Natural infection of cattle with an atypical `HoBi'-like pestivirus – Implications for BVD control and for the safety of biological products

et al. 2007

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-During a study on Bovine Viral Diarrhoea (BVD) epidemiology in Thailand, a pestivirus was detected in serum from a calf. Comparative nucleotide sequence analysis showed that this virus was closely related to a recently described atypical pestivirus (D32/00_'HoBi') that was first isolated from a batch of foetal calf serum collected in Brazil. The results from virus neutralisation tests performed on sera collected from cattle in the herd of the infected calf, showed that these cattle had markedly higher antibody titres against the atypical pestivirus 'HoBi' than against Bovine Viral Diarrhoea Virus types 1 and 2, or Border Disease Virus. The results also supported, consequently, the results from the molecular analysis, and demonstrated that a 'HoBi'-like pestivirus had been introduced to, and was now circulating in the herd. This study is the first to report a natural infection in cattle with a virus related to this atypical pestivirus, and it suggests that this group of pestiviruses may already be spread in cattle populations. The findings have implications for BVD control and for the biosafety of vaccines and other biological products produced with foetal calf serum. Consequently, these atypical pestiviruses should be included in serological assays, and any diagnostic assay aimed at detection of pestiviruses in biological products or animals should be tested for its ability to detect them.BVDV / pestivirus / control / biosafety

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Section: Molecular Methodsmentioning

confidence: 99%

Natural infection of cattle with an atypical `HoBi'-like pestivirus – Implications for BVD control and for the safety of biological products

et al. 2007

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“…CSFV specific primers were designed considering sequences of the 5'NTR of the genome of 55 different pestivirus strains, available in GenBank or in the database of CSF European reference laboratory of Hanover (Germany), that includes 37 CSFV representing subgroups described by Lowings [20]: 1.1, 1.2, 2.1, 2.2, 2.3 and disparate virus outgrouped [12,13,15,25,27,[31][32][33], fourteen BVDV belonging to types I and II [5,8,32] and four BDV isolates [4,29]. Sequences were aligned, using Clustal W software, and five oligonucleotides were primarily selected from highly conserved sequences among the different CSFV strains but showing high divergence with the other pestiviruses.…”

Section: Primers and Restriction Endonuclease Selectionmentioning

confidence: 99%

A highly sensitive and specific gel-based multiplex RT-PCR assay for the simultaneous and differential diagnosis of African swine fever and Classical swine fever in clinical samples

Agüero

Fernández²,

Romero

et al. 2004

Vet. Res.

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-The development and standardisation of a novel, highly sensitive and specific one-step hot start multiplex RT-PCR assay is presented for the simultaneous and differential diagnosis of African swine fever (ASF) and Classical swine fever (CSF). The method uses two primer sets, each one specific for the corresponding virus, amplifying DNA fragments different in length, allowing a gel-based differential detection of the PCR products. Universal detection of ASF and CSF virus strains was achieved through selection of primers in conserved viral genome regions. The detection range was confirmed by analysis of a large collection of isolates of the two viruses. The high specificity of the assay was proven by testing related viruses, uninfected cell line cultures and healthy pig tissues. Additional confirmatory tests of the ASF and CSF virus amplicon specificity, based on restriction endonuclease analysis with BsmA I or Ban II, respectively, are also described. The analysis of whole blood and serum samples from experimentally infected animals proved the usefulness of the method for an early diagnosis of both diseases, even before the appearance of the first clinical signs. A study of 150 positive field samples from several ASF and CSF outbreaks showed the suitability of this method for a rapid (less than five hours), sensitive and specific differential diagnosis in clinical samples. In addition, a highly sensitive and specific uniplex RT-PCR for CSFV was also developed and standardised as a powerful tool for fast and early diagnosis of the disease.

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“…BVDV is an enveloped virus with an uncapped and non-polyadenylated positive-stranded RNA genome of about 12.5 kb in size. The genome comprises a single open reading frame (ORF) that is flanked by 5' and 3' untranslated regions (UTRs) [11,12,14]. The 5'UTR function is a so-called internal ribosome entry site (IRES) that promotes cap-independent translation initiation [30,31].…”

Section: Introductionmentioning

confidence: 99%

Retrospective genome analysis of a live vaccine strain of bovine viral diarrhea virus

Bálint¹,

Baule

Pálfi³

et al. 2005

Vet. Res.

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-A live bovine viral diarrhea (BVDV) vaccine, marketed as a derivate of the Oregon C24V strain, was used between the end of the 1960s and the beginning of the 1990s in Central Europe. Since laboratory investigations of mucosal disease cases in vaccinated animals suggested recombinations between the vaccine and wild type variants of BVDV, and recombinational nucleotide sequences seemed distinct from BVDV Oregon C24V, the aim of the present retrospective study was to analyze the genomes of pre-registration (termed here BVDV-Xpre) and of marketed (BVDV-X) batches of the vaccine. The results of the complete genome analysis of BVDV-Xpre confirmed that the original virus strain used at the start of the vaccine production was Oregon C24V. Surprisingly, the analysis of the complete nucleotide sequence of the BVDV-X marketed vaccine revealed that this strain belongs to the BVDV 1b subgroup, with a 93.7% nucleotide sequence homology to BVDV reference strain Osloss. The homology to BVDV Oregon C24V was significantly lower (77.4%), and a thorough sequence scanning showed that the genome of BVDV-X had not derived from Oregon C24V. These data indicate the very likely scenario that a strain different to Oregon C24V was picked up during the in vitro or in vivo passages for vaccine development. Despite of the virus-switch, the BVDV-X vaccine continuously maintained its innocuity and efficacy, as proven by the regular quality testing data, and the presence of the foreign virus remained unnoticed over many years. The results of this work emphasize that the contamination of commercially available live vaccines with exogenous BVDV strains is a real risk factor, and a unequivocal analysis, including molecular methods, is needed to verify their authenticity. bovine viral diarrhea virus / vaccine / strain switch / control

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Molecular cloning and nucleotide sequence of the pestivirus bovine viral diarrhea virus

Cited by 448 publications

References 19 publications

Natural infection of cattle with an atypical `HoBi'-like pestivirus – Implications for BVD control and for the safety of biological products

Natural infection of cattle with an atypical `HoBi'-like pestivirus – Implications for BVD control and for the safety of biological products

A highly sensitive and specific gel-based multiplex RT-PCR assay for the simultaneous and differential diagnosis of African swine fever and Classical swine fever in clinical samples

Retrospective genome analysis of a live vaccine strain of bovine viral diarrhea virus

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