Molecular Cloning and Partial Characterization of a Peroxidase Gene Expressed in the Roots of<i>Portulaca oleracea</i>cv., One Potentially Useful in the Remediation of Phenolic Pollutants

Matsui, Toshimitsu; Nomura, Yuki; Takano, Mai; Imai, Sofue; Nakayama, Hideki; Miyasaka, Hitoshi; Okuhata, Hiroshi; Tanaka, Satoshi; Matsuura, Hideyuki; Harada, Kaoru; Bamba, Takeshi; Hirata, Kazumasa; Kato, Ko

doi:10.1271/bbb.100823

Cited by 16 publications

(7 citation statements)

References 30 publications

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“…Soares et al, [28] have suggested that the scarce accumulation of NP in plants could be related to its hydrophobic nature. In addition, according to Gonzàlez et al [54] and Matsui et al [55], NP similarly to other phenolic compounds could be bound to polysaccharides and proteins of cell walls on the root surface. In another study, Gattullo et al [56] have tested the capability of the freshwater green alga Monoraphidium braunii (Nägeli) to remove BPA at concentrations of 2, 4, and 10 mg L -1 , also in presence of NOM at concentrations of 2, 5 and 20 mg L -1 .…”

Section: The Effects Of Natural Organic Matter On Phytoremediation Ofmentioning

confidence: 96%

Phytodecontamination of Water Systems from Phenolic Endocrine Disruptors and the Regulation Role of Natural Organic Matter

Loffredo

Traversa²

2016

TOBIOTJ

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In the last decades an increasing number of natural and synthetic compounds have been recognized as endocrine disruptors (EDs) because of their hormone-like activity and capacity to alter the normal hormonal functions of animals and humans. Among EDs, there are phenolic compounds widely present in terrestrial and aquatic systems, such as bisphenol A, 4-nonylphenol (NP), 4-tert-octylphenol, estrone, ethynilestradiol and so on. Estrogenic effects of these molecules have been ascertained on mollusks, crustaceans, fishes, amphibians and mammals starting from concentrations of 1 μgL−1. Thus, the removal of EDs from polluted media is a priority goal in order to avoid risks for the ecosystem health. Nowadays, several physico-chemical methods are mainly used for the removal of EDs from liquid and solid matrices. Nevertheless, these methods are expensive, difficult to apply and may produce a negative impact on the environment. Recently, most of studies on soil and water remediation from EDs address more sustainable techniques using bacteria, fungi, microbial enzymes and plants. Phytoremediation uses photoautotrophic organisms to uptake, transform, volatilize or stabilize pollutants present in waters, sediments, soils and atmosphere. As this technology is solar driven and exploits natural sources, it is consequently environmentally safe and cost-effective. A fundamental role in the phytoremediation process is played by natural organic molecules, mainly dissolved organic matter and humic substances. These compounds are ubiquitous in all terrestrial and aquatic environments and they interact at various extent with all contaminants deriving from agricultural, industrial and urban activities. Natural organic matter has a relevant biological activity and may also regulate the decontamination capacity of plants and other organisms, such as algae and fungi. In this review, some results of phytodecontamination studies conducted using herbaceous plant species which are presented and discussed. Further, the modulation role of natural organic matter on the phytodecontamination process is highlighted.

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Section: The Effects Of Natural Organic Matter On Phytoremediation Ofmentioning

confidence: 96%

Phytodecontamination of Water Systems from Phenolic Endocrine Disruptors and the Regulation Role of Natural Organic Matter

Loffredo

Traversa²

2016

TOBIOTJ

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“…Gonzàlez et al [54] and Matsui et al [55], NP similarly to other phenolic compounds could be bound to polysaccharides and proteins of cell walls on the root surface.…”

Section: The Effects Of Natural Organic Matter On Phytoremediation Ofmentioning

confidence: 98%

Phytodecontamination of Water Systems from Phenolic Endocrine Disruptors and the Regulation Role of Natural Organic Matter

Loffredo¹,

Traversa²

2015

TOBIOTJ

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“…en, PoPPO1 and PoPPO3 genes were spliced out with XbaI and SalI in the 3′-UTR and inserted into the ADH 5′-UTR:: PoPRX2::HA:strep vectors (Matsui et al 2011) using the same restriction sites. PoPPO2 and PoPPO4 genes were spliced out with XbaI and SacI from pUC118 and inserted into the pBI121 vector (Clontech, Palo Alto, CA) using the same restriction sites.…”

Section: Construction Of Expression Vectors For Poppo Genesmentioning

confidence: 99%

Isolation of polyphenol oxidase genes from Portulaca oleracea and evaluation of their ability to metabolize endocrine-disrupting chemicals

Kaneda

Matsui

Tomiyasu

et al. 2012

Plant Biotechnology

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Portulaca (Portulaca oleracea cv.), a garden plant, e ciently removes endocrine-disrupting chemicals (EDCs) including bisphenol A (BPA) from hydroponic solution. We hypothesized that polyphenol oxidase (PPO) was involved in the initial steps of detoxifying EDCs in portulaca roots. In order to elucidate the molecular basis of portulaca's ability to metabolize EDCs, we rst isolated ve PPO genes (PoPPO1-5) that were expressed mainly in portulaca roots. Among these genes, PoPPO2, PoPPO4 and PoPPO5 were introduced into cultured tobacco cells and expressed as active forms. We found that crude extracts from the cells expressing PoPPO2, PoPPO5, and to a lesser extent PoPPO4, could metabolize BPA. In addition, we found that the BPA metabolites from crude extracts of cells expressing PoPPO2, PoPPO4 and PoPPO5 were identical to those of portulaca. Moreover, PoPPO2 and PoPPO5 also caused hydroxylation of octylphenol, nonylphenol and 17β-estradiol. erefore, these results strongly suggest that PoPPOs signi cantly contribute to the superior ability of portulaca to metabolize EDCs.

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Molecular Cloning and Partial Characterization of a Peroxidase Gene Expressed in the Roots ofPortulaca oleraceacv., One Potentially Useful in the Remediation of Phenolic Pollutants

Cited by 16 publications

References 30 publications

Phytodecontamination of Water Systems from Phenolic Endocrine Disruptors and the Regulation Role of Natural Organic Matter

Phytodecontamination of Water Systems from Phenolic Endocrine Disruptors and the Regulation Role of Natural Organic Matter

Phytodecontamination of Water Systems from Phenolic Endocrine Disruptors and the Regulation Role of Natural Organic Matter

Isolation of polyphenol oxidase genes from Portulaca oleracea and evaluation of their ability to metabolize endocrine-disrupting chemicals

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