Molecular cloning and properties of the chicken leptin-receptor (CLEPR) gene

Horev, Guy; Einat, Paz; Aharoni, Tomer; Eshdat, Yuval; Friedman-Einat, Miriam

doi:10.1016/s0303-7207(00)00205-7

Cited by 112 publications

(71 citation statements)

References 47 publications

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“…Ohkubo et al (2000) reported the greatest mRNA expression in the ovary and brain, with less abundance in the liver, kidney and intestine. A similar pattern was observed by Horev et al (2000) in tissue mRNA expression levels, with the greatest activity in the brain (hypothalamus), followed by lung, kidney, fat, and liver. This pattern is somewhat similar to what we observed in this study in that in general, the greatest radiolabeled leptin activity uptake was in the kidney, spleen and lungs, and intestine.…”

Section: Discussionsupporting

confidence: 77%

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Plasma clearance and tissue distribution of radiolabeled leptin in the chicken

McMurtry

2004

Comparative Biochemistry and Physiology - Part A: Molecular &am

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Leptin is an adipose and liver tissue-derived secreted protein in chickens that has been implicated in the regulation of food intake and whole-body energy balance. In this study, the metabolic clearance and tissue uptake of leptin were examined in the chicken (Gallus gallus). Four-week-old broiler males were infused with 125 I-labeled mouse leptin. Chromatography of radiolabeled leptin in plasma produced two peaks, one at 16 kDa (free leptin) and a free iodine peak. No leptin binding protein in blood was detected. Leptin was cleared with a half-life estimate of 23 min. In order to investigate the tissue distribution and uptake of radiolabeled leptin, multiple tissues were removed from infused birds at 15 and 240 min post-infusion, and trichloroacetic acid (TCA)-precipitable radioactivity was determined. The amounts of radioactivity at 15 min post-infusion in the tissues in rank order were: kidney, testis, lung, spleen, heart, liver, small and large intestine, gizzard, pancreas, bursa, leg and breast muscle, adrenals, and brain. A slightly different pattern of distribution was observed at 240 min postinfusion. We conclude from these studies that unlike mammals, no circulating leptin binding protein is present in chickens. Leptin is metabolized and cleared very rapidly from blood by the kidney. Published by Elsevier Inc.

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Section: Discussionsupporting

confidence: 77%

“…To date, only the long form of the tissue leptin receptor has been identified in birds (Ohkubo et al, 2000;Horev et al, 2000;Richards et al, 2001). The leptin receptor has also been identified in cells derived from a Leghorn male hepatoma cell line (Cassy et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Plasma clearance and tissue distribution of radiolabeled leptin in the chicken

McMurtry

2004

Comparative Biochemistry and Physiology - Part A: Molecular &am

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“…A relatively high level of mRNA expression was observed in both strains under conditions of being fed ad libitum, followed by a markedly reduced level after 1 week of feed restriction. Given that a much higher fat content is typical to broiler chicks even at this young age (Griffin et al 1991), and assuming that adiposesecreted proteins elicit brain response in chickens (Horev et al 2000, Ohkubo et al 2000 similarly to that described for mammals (Ahima & Flier 2000, Saltiel 2001), a much higher hypothalamic POMC level would be expected under the assumption of similar sensitivity to these fat-secreted signals.…”

Section: Discussionmentioning

confidence: 94%

“…Total RNA was isolated from the hypothalamus as described previously (Horev et al 2000). Each sample was pooled from four birds.…”

Section: Mrna Analysismentioning

confidence: 99%

The melanocortin circuit in obese and lean strains of chicks

Hen

Yosefi

Simchaev

et al. 2006

Journal of Endocrinology

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Agonists of membranal melanocortin 3 and 4 receptors (MC3/4Rs) are known to take part in the complex control mechanism of energy balance. In this study, we compared the physiological response to an exogenous MC3/4R agonist and the hypothalamic expression of proopic melanocortin (POMC) gene, encoding few MC3/4R ligands, between broiler and layer chicken strains. These strains, representing the two most prominent commercial strains of chickens grown for meat (broilers) and egg production (layers), differ in their food intake, fat accumulation, and reproductive performance and, therefore, form a good model of obese and lean phenotypes, respectively. A single i.v. injection of the synthetic peptide melanotan-II (MT-II; 1 mg/kg body weight) into the wing vein of feed-restricted birds led to attenuation of food intake upon exposure to feeding ad libitum in both broiler and layer chickens. A study of the POMC mRNA encoding the two prominent natural MC3/4R agonists, a-MSH and ACTH, also revealed a general similarity between the strains. Under feeding conditions ad libitum, POMC mRNA levels were highly similar in chicks of both strains and this level was significantly reduced upon feed restriction. However, POMC mRNA down-regulation upon feed restriction was more pronounced in layers than in broilers. These results suggest: (i) a role for MC3/4R agonists in the control of appetite; (ii) that the physiological differences between broilers and layers are not related to unresponsiveness of broiler chickens to the satiety signal of MC3/4R ligands. Therefore, these findings suggest that artificial activation of this circuit in broiler chicks could help to accommodate with their agricultural shortcomings of overeating, fattening, and impaired reproduction.

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“…Em galinhas, o cDNA do receptor da leptina é composto por 1148 aminoácidos, e sua seqüência é 62%, 61% e 59% idêntica ao receptor da leptina em humanos, bovinos e roedores, respectivamente (HOREV et al, 2000). Através de análise de segregação, Dunn et al (2000) localizaram o gene do receptor da leptina no cromossomo 8 da galinha.…”

Section: Gene Da Leptina E Seu Receptor Em Avesunclassified

Identificação de polimorfismos no gene da leptina e de seu receptor em duas linhagens de aves e associação com características de desempenho e carcaça

Ninov¹

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Molecular cloning and properties of the chicken leptin-receptor (CLEPR) gene

Cited by 112 publications

References 47 publications

Plasma clearance and tissue distribution of radiolabeled leptin in the chicken

Plasma clearance and tissue distribution of radiolabeled leptin in the chicken

The melanocortin circuit in obese and lean strains of chicks

Identificação de polimorfismos no gene da leptina e de seu receptor em duas linhagens de aves e associação com características de desempenho e carcaça

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