Molecular cloning and sequence analysis of putative chicken prolactin cDNA

Hanks, Mark C.; Alonzi, J A; Sharp, P J; Sang, Helen

doi:10.1677/jme.0.0020021

Cited by 42 publications

(10 citation statements)

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“…Gaps have been introduced to obtain maximal homology. Sequences for man (Cooke, Coit, Shine et al 1981), pig (Kato et al 1990), ox (Sasavage, Nilson, Holowitz & Rottman, 1982), rat (Cooke, Coit, Weiner et al 1980), mouse (Linzer & Talamantes, 1985), chicken (Hanks, Alonzi, Sharp & Sang, 1989), salmon (Song, Trinh, Hew et al 1988) and bullfrog (present study) are shown.…”

Section: Discussionmentioning

confidence: 54%

Molecular cloning and nucleotide sequence analysis of complementary DNA for bullfrog prolactin

Takahashi¹,

Yoshihama²,

Kikuyama³

et al. 1990

Journal of Molecular Endocrinology

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A prolactin cDNA was cloned from a cDNA expression library constructed from total RNA of bullfrog (Rana catesbeiana) adenohypophyses by immunoscreening with antiserum against bullfrog prolactin. The cDNA clone thus obtained contained a 249 bp insert. Using this clone as a probe, plaque hybridizations were performed and two additional clones obtained. These clones had a polyadenylation site different from that of the first obtained clone, suggesting that the 3'-untranslated sequence was heterogeneous in length. The longest clone contained 830 bp, which encoded part of the signal peptide and the entire sequence of mature prolactin. The deduced amino acid sequence was in good accord with that determined by direct protein sequencing of purified bullfrog prolactin. The length of the bullfrog prolactin mRNA was estimated by Northern blot analysis to be about 1.0 kb. Homologies of prolactin nucleotide and amino acid sequences between bullfrog and other vertebrates were 64 and 65% for man, 66 and 68% for pig, 61 and 52% for rat, 69 and 74% for chicken, and 50 and 35% for salmon respectively. Highly conserved regions reported for mammalian prolactins also existed in bullfrog prolactin. Homologies of nucleotide and amino acid sequences between prolactin and GH of bullfrog origin were 49 and 25% respectively. Using the cDNA, the content of prolactin mRNA in the pituitary glands of metamorphosing tadpoles was measured. Prolactin mRNA levels rose at the mid-climax stage, suggesting that the increase in plasma and pituitary prolactin levels known to occur at the climax stage accompanies the increase in prolactin synthesis.

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Section: Discussionmentioning

confidence: 54%

Molecular cloning and nucleotide sequence analysis of complementary DNA for bullfrog prolactin

Takahashi¹,

Yoshihama²,

Kikuyama³

et al. 1990

Journal of Molecular Endocrinology

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“…Because nucleotide sequences are higly conserved between chicken and quail, chicken and quail probes are widely used for both species in methods such as Northern hybridization, Southern hybridization, in situ hybridization, chromosomal mapping using FISH, and microarrays (17,(35)(36)(37)(38)(39)(40). Therefore, we used chicken DNA sequences for in situ hybridization with 33 P-labeled (17).…”

Section: Discussionmentioning

confidence: 99%

A mammalian neural tissue opsin (Opsin 5) is a deep brain photoreceptor in birds

Nakane

Ikegami

Ono

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

267

242

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It has been known for many decades that nonmammalian vertebrates detect light by deep brain photoreceptors that lie outside the retina and pineal organ to regulate seasonal cycle of reproduction. However, the identity of these photoreceptors has so far remained unclear. Here we report that Opsin 5 is a deep brain photoreceptive molecule in the quail brain. Expression analysis of members of the opsin superfamily identified as Opsin 5 (OPN5; also known as Gpr136, Neuropsin, PGR12, and TMEM13) mRNA in the paraventricular organ (PVO), an area long believed to be capable of phototransduction. Immunohistochemistry identified Opsin 5 in neurons that contact the cerebrospinal fluid in the PVO, as well as fibers extending to the external zone of the median eminence adjacent to the pars tuberalis of the pituitary gland, which translates photoperiodic information into neuroendocrine responses. Heterologous expression of Opsin 5 in Xenopus oocytes resulted in light-dependent activation of membrane currents, the action spectrum of which showed peak sensitivity (λ max ) at ∼420 nm. We also found that short-wavelength light, i.e., between UV-B and blue light, induced photoperiodic responses in eye-patched, pinealectomized quail. Thus, Opsin 5 appears to be one of the deep brain photoreceptive molecules that regulates seasonal reproduction in birds.circadian rhythms | Japanese quail | photoperiodism | paraventricular organ | cerebrospinal fluid-contacting neuron

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“…Both the gene encoding PRL and its cDNA had been cloned in chickens, turkeys and domestic ducks (Hanks et al . 1989; Watahiki et al .…”

Section: Introductionmentioning

confidence: 99%

Cloning of PRL and VIP cDNAs of the Java sparrow (Padda oryzivora)

Hiyama

Sato

Zadworny

et al. 2009

Animal Science Journal

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Complementary DNA (cDNA) of prolactin (PRL) and vasoactive intestinal polypeptide (VIP) of the Java sparrow were cloned and sequenced. The proximal region of the PRL promoter was also identified. Java sparrow PRL was found to have 88.3, 88.3, and 89.1% sequence identity at the cDNA level to PRL of chicken, turkey, and duck, respectively. The predicted amino acid sequence had an overall similarity with a comparable region of chicken (91.4%), turkey (88.9%) and duck (92.0%) PRL. Based on the cDNA sequence and genomic structure of the chicken PRL gene, the proximal promoter was characterized. Sequence analysis of the proximal region of Java sparrow PRL promoter revealed a high degree of similarity to that of chicken, turkey and duck PRL promoters. Moreover, cDNA of prepro-VIP was also cloned and sequenced. Java sparrow prepro-VIP shows high similarity to chicken and turkey prepro-VIP. However, the region upstream of the 5' untranslated region of Java sparrow prepro-VIP did not show similarity to that of chicken. These results suggest that the mechanisms, which regulate expression of the VIP gene, may be different between precocial and altricial birds, but expression of the PRL gene may be widely conserved in avian species.

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Molecular cloning and sequence analysis of putative chicken prolactin cDNA

Cited by 42 publications

References 0 publications

Molecular cloning and nucleotide sequence analysis of complementary DNA for bullfrog prolactin

Molecular cloning and nucleotide sequence analysis of complementary DNA for bullfrog prolactin

A mammalian neural tissue opsin (Opsin 5) is a deep brain photoreceptor in birds

Cloning of PRL and VIP cDNAs of the Java sparrow (Padda oryzivora)

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