Molecular cloning and sequence analysis of the 3′-terminal region of iris severe mosaic virus RNA

Vlugt, C.I.M. van der; Langeveld, S.A.; Goldbach, Rob

doi:10.1007/bf01321068

Cited by 7 publications

(2 citation statements)

References 41 publications

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“…To our knowledge, it is the first report of ISMV occurrence in Iran. We found that ISMV-Ir was not able to infect the number of mechanically inoculated test plant species, which was similar to the results of previous research carried out by Barnett et al (1971), Brunt et al (1980Brunt et al ( , 1988, Derks and Hollinger (1986), Van der Vlugt et al (1994). Only a couple of tested plant species (C. vernus and I. domestica) are diagnostically susceptible to ISMV.…”

Section: Rt-pcr Sequencing and Phylogenetic Analysissupporting

confidence: 91%

Detection and molecular characterization of the Iris severe mosaic virus-Ir isolate from Iran

Nateqi¹,

Habibi²,

Dizadji³

et al. 2015

Journal of Plant Protection Research

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Iris belongs to the Iridaceae family. It is one of the most important pharmaceutical and ornamental plants in the world. To assess the potyvirus incidence in natural resources of iris plants in Iran, Antigen Coated-Plate ELISA (ACP-ELISA) was performed on 490 symptomatic rhizomatous iris leaf samples, which detected the potyvirus in 36.7% of the samples. Genomic 3' end of one mechanically non-transmitted potyvirus isolate, comprising a 3' untranslated region (390 bp) and C-terminus of the coat protein (CP) gene (459 bp), was amplified by reverse transcription polymerase chain reaction (RT-PCR), which was ligated into pTG19-T vector.The nucleotide sequence of amplicons was compared with related sequences, using Blastn software available at NCBI GenBank, and showed the highest similarity with Iris severe mosaic virus (ISMV) isolates. The nucleotide and deduced amino acid sequence of the CP C-terminus region was more than 83% identical with other ISMV isolates, therefore this isolate was designated as ISMV-Ir. This new ISMV isolate is closely related to the Chinese ISMV-PHz in phylogenetic analysis, based on the partial nucleotide and deduced amino acid sequence of the CP region. This is the first report of ISMV occurrence on Iris spp. in Iran.

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