Molecular cloning and sequence analysis of two carbonic anhydrase in the swimming crab Portunus trituberculatus and its expression in response to salinity and pH stress

Pan, Luqing; Hu, Dongxu; Liu, Changchun; Hu, Yifei; Liu, Shengnan

doi:10.1016/j.gene.2015.10.049

Cited by 27 publications

(15 citation statements)

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“…In addition, the expression of genes encoding NKA, CAc, and NHE in euryhaline crabs increases under hypo-osmotic conditions (Henry et al, 2003;Liu et al, 2015;Lovett, et al, 2006b;Lucu & Flik, 1999;Lucu & Towle, 2003;Pan et al, 2016). This study revealed that the expression of these genes was also enhanced in H. tridens and M.…”

Section: Role Of Ambient Minor Cations Under Isosmotic Conditions Wmentioning

confidence: 59%

“…CAc in chloride cells catalyzes the formation of H + and HCO 3 ‐ HCO from H 2 O and CO 2 , and the derived H + supports Na + uptake through activation of NHE located in the apical membrane (Charmantier et al., 2009; Freire et al., 2008; Griffith, 2017; Henry et al., 2012). In addition, the expression of genes encoding NKA, CAc, and NHE in euryhaline crabs increases under hypo‐osmotic conditions (Henry et al., 2003; Liu et al., 2015; Lovett, et al., 2006; Lucu & Flik, 1999; Lucu & Towle, 2003; Pan et al., 2016). This study revealed that the expression of these genes was also enhanced in H. tridens and M. japonicus under hypo‐osmotic conditions (8.6 mmol/L NaCl solution) (Figure 5), consistent with previous studies.…”

Section: Discussionmentioning

confidence: 99%

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Hemolymph composition, gene expressions in the gills, and thus the survival of euryhaline crabs are controlled by ambient minor cations according to osmotic condition‐dependent manner

Yamaguchi

Soga

2020

Ecology and Evolution

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Section: Role Of Ambient Minor Cations Under Isosmotic Conditions Wmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Hemolymph composition, gene expressions in the gills, and thus the survival of euryhaline crabs are controlled by ambient minor cations according to osmotic condition‐dependent manner

Yamaguchi

Soga

2020

Ecology and Evolution

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“…CAc in chloride cells catalyzes the formation of H + and HCO 3 from H 2 O and CO 2 , and the derived H + supports Na + uptake through activation of NHE located in the apical membrane (Freire et al, 2008;Charmantier et al, 2009;Henry et al, 2012;Griffith, 2017). In addition, the expression of genes encoding NKA, CAc, and NHE in euryhaline crabs increases under hypo-osmotic conditions (Lucu and Flik, 1999;Henry et al, 2003;Lucu and Towle, 2003;Lovett et al, 2006b;Liu et al, 2015;Pan et al, 2016). This study revealed that the expression of these genes was also enhanced in H. tridens and M. japonicus under hypo-osmotic conditions (8.6 mmol/L NaCl solution) ( Fig.…”

Section: 2| Role Of Ambient Minor Cations Under Isosmotic Conditionmentioning

confidence: 99%

Hemolymph composition, gene expressions in the gills, and thus the survival of euryhaline crabs are controlled by ambient minor cations according to osmotic condition-dependent manner

Yamaguchi

Soga

2020

Preprint

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Na+ and Cl-are the most abundant dissolved ions in seawater, constituting~85% of total ions. They significantly affect the osmolality of body fluids of marine invertebrates. Seawater also contains minor ions such as Mg2+, Ca2+, K+, and SO42-, but their effects on marine organisms are unclear. This study analyzed the effects of Mg2+, Ca2+, and K+ (ambient minor cations) on survival, hemolymph ionic composition, and gene expression in the gills of three euryhaline crabs: Helice tridens, Macrophthalmus japonicus, and Chiromantes dehaani. Ambient minor cations were required for survival of H. tridens and M. japonicus under isosmotic conditions with seawater. The ambient minor cations also affected the osmolality and ionic composition of hemolymph by regulating expressions of specific genes in the gills required for Na+ uptake, such as Na+/K+ ATPase, cytoplasmic carbonic anhydrase, and Na+/H+ exchanger. Administration of carbonic anhydrase and Na+/H+ exchanger inhibitors increased the survival rate even if ambient minor cations did not exist. In contrast, under hypo-osmotic conditions, ambient minor cations had different effects on crabs; a lethal effect on M. japonicus, and an increase of the hemolymph K+ concentration in H. tridens and M. japonicus. It is thus concluded that the effects of ambient minor cations are osmolality-dependent. In contrast, in C. dehaani, the hemolymph ionic composition and survival rate were hardly affected by ambient minor cations, probably reflecting the habitat of this species. These results strongly indicated that C. dehaani is less susceptive to ambient minor cations compared to H. tridens and M. japonicus.

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“…Its production in China amounted to 100,000 tons in 2015 and 600,000 tons in 2017, and the production capacity is steadily growing [ 17 , 18 ]. There are few studies on the processing or utilization of P. trituberculatus , while several papers have focused on its gene sequence analysis or aquaculture [ 19 , 20 ]. Recently, we revealed that egg oil isolated from P. trituberculatus ( Pt -egg oil) contains abundant phospholipids and improved hyperglycemia and hyperinsulinemia in high-fat-diet (HFD)-fed mice [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Phosphatidylserine from Portunus trituberculatus Eggs Alleviates Insulin Resistance and Alters the Gut Microbiota in High-Fat-Diet-Fed Mice

et al. 2020

Marine Drugs

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Portunus trituberculatus eggs contain phospholipids, whose components and bioactivity are unclear. Here, we investigated the fatty acid composition of phosphatidylserine from P. trituberculatus eggs (Pt-PS). Moreover, its effects on insulin resistance and gut microbiota were also evaluated in high-fat-diet-fed mice. Our results showed that Pt-PS accounted for 26.51% of phospholipids and contained abundant polyunsaturated fatty acids (more than 50% of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)). Animal experiments indicated that Pt-PS significantly decreased body weight and adipose weight gain, improved hyperglycemia and hyperinsulinemia, mitigated insulin resistance, and regulated circulatory cytokines. Pt-PS activated insulin receptor substrate 1 (IRS1) and increased the levels of IRS1-associated phosphatidylinositol 3-hydroxy kinase (PI3K), phosphorylated protein kinase B (Akt) protein, and plasma membrane glucose transporter 4 protein. Furthermore, Pt-PS modified the gut microbiota, inducing, especially, a dramatic decrease in the ratio of Firmicutes to Bacteroidetes at the phylum level, as well as a remarkable improvement in their subordinate categories. Pt-PS also reduced fecal lipopolysaccharide concentration and enhanced fecal acetate, propionate, and butyrate concentrations. Additionally, the effects of Pt-PS on alleviation of insulin resistance and regulation of intestinal bacteria were better than those of phosphatidylserine from soybean. These results suggest that Pt-PS mitigates insulin resistance by altering the gut microbiota. Therefore, Pt-PS may be developed as an effective food supplement for the inhibition of insulin resistance and the regulation of human gut health.

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Molecular cloning and sequence analysis of two carbonic anhydrase in the swimming crab Portunus trituberculatus and its expression in response to salinity and pH stress

Cited by 27 publications

References 56 publications

Hemolymph composition, gene expressions in the gills, and thus the survival of euryhaline crabs are controlled by ambient minor cations according to osmotic condition‐dependent manner

Hemolymph composition, gene expressions in the gills, and thus the survival of euryhaline crabs are controlled by ambient minor cations according to osmotic condition‐dependent manner

Hemolymph composition, gene expressions in the gills, and thus the survival of euryhaline crabs are controlled by ambient minor cations according to osmotic condition-dependent manner

Phosphatidylserine from Portunus trituberculatus Eggs Alleviates Insulin Resistance and Alters the Gut Microbiota in High-Fat-Diet-Fed Mice

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