Molecular cloning and subcellular localization of six HDACs and their roles in response to salt and drought stress in kenaf (Hibiscus cannabinus L.)

Fan, Wei; Tang, Danfeng; Li, Zengqiang; Kashif, Muhammad; Khan, Aziz; Lü, Hai; Jia, Ruixing; Chen, Peng

doi:10.1186/s40659-019-0227-6

Cited by 36 publications

(15 citation statements)

References 52 publications

(47 reference statements)

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“…This process is catalyzed by histone acetyltransferases (HaTs) and histone deacetyltransferases (Hdacs), which are responsible for adding and removing acetyl groups from histone tails, respectively. Hdac-mediated acetylation is associated with ischemic heart disease (11). application of trichostatin a (TSa), a class i and class ii Hdac inhibitor alters the response to myocardial ischemic injury in the heart and limited areas of Mi (12).…”

Section: Introductionmentioning

confidence: 99%

H3K14 hyperacetylation‑mediated c‑Myc binding to the miR‑30a‑5p gene promoter under hypoxia postconditioning protects senescent cardiomyocytes from hypoxia/reoxygenation injury

Zhang

Wang

et al. 2021

Mol Med Rep

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our previous study reported that microrna (mir)-30a-5p upregulation under hypoxia postconditioning (HPostc) exert a protective effect on aged H9c2 cells against hypoxia/reoxygenation injury via dna methyltransferase 3B-induced dna hypomethylation at the mir-30a-5p gene promoter. This suggests that mir-30a-5p may be a potential preventative and therapeutic target for ischemic heart disease in aged myocardium. The present study aimed to investigate the underlying mechanisms of mir-30a-5p transcription in aged myocardium in ischemic heart disease. cardiomyocytes were treated with 8 mg/ml d-galactose for 9 days, and then exposed to hypoxic conditions. cell viability was determined using a cell viability assay. expression levels of histone deacetylase 2 (Hdac2), lc3B-ii/i, beclin-1 and p62 were detected via reverse transcription-quantitative Pcr and western blotting. chromatin immunoprecipitation-Pcr and luciferase reporter assays were performed to evaluate the effect of c-Myc binding and activity on the mir-30a-5p promoter in senescent cardiomyocytes following HPostc. it was found that HPostc enhanced the acetylation levels of H3K14 at the mir-30a-5p gene promoter in senescent cardiomyocytes, which attributed to the decreased expression of Hdac2. in addition, c-Myc could positively regulate mir-30a-5p transcription to inhibit senescent cardiomyocyte autophagy. Mechanically, it was observed that increased H3K14 acetylation level exposed to romidepsin facilitated c-Myc binding to the mir-30a-5p gene promoter region, which led to the increased transcription of mir-30a-5p. Taken together, these results demonstrated that Hdac2-mediated H3K14 hyperacetylation promoted c-Myc binding to the mir-30a-5p gene promoter, which contributed to HPostc senescent cardioprotection.

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Section: Introductionmentioning

confidence: 99%

H3K14 hyperacetylation‑mediated c‑Myc binding to the miR‑30a‑5p gene promoter under hypoxia postconditioning protects senescent cardiomyocytes from hypoxia/reoxygenation injury

Zhang

Wang

et al. 2021

Mol Med Rep

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“…In addition, high acetylation levels of H3K and low acetylation levels of H3K27 and H4K5 were observed following a lower PEG treatment. Meanwhile, acetylation levels of H3K9, H3K27 and H4K5 were all reduced in response to a higher PEG treatment [74].…”

Section: Hdacsmentioning

confidence: 95%

“…For example, DoHDA10 and DoHDT4 were highly up-regulated after PEG-mediated drought treatment, while other HDAC genes were not changed [52]. Wei et al studied the roles of six HDACs in response to drought stress in kenaf and found up-regulation of all these HDACs after PEG treatments [74]. In addition, high acetylation levels of H3K and low acetylation levels of H3K27 and H4K5 were observed following a lower PEG treatment.…”

Section: Hdacsmentioning

confidence: 99%

Histone Acetylation Changes in Plant Response to Drought Stress

Gao

et al. 2021

Genes

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Drought stress causes recurrent damage to a healthy ecosystem because it has major adverse effects on the growth and productivity of plants. However, plants have developed drought avoidance and resilience for survival through many strategies, such as increasing water absorption and conduction, reducing water loss and conversing growth stages. Understanding how plants respond and regulate drought stress would be important for creating and breeding better plants to help maintain a sound ecosystem. Epigenetic marks are a group of regulators affecting drought response and resilience in plants through modification of chromatin structure to control the transcription of pertinent genes. Histone acetylation is an ubiquitous epigenetic mark. The level of histone acetylation, which is regulated by histone acetyltransferases (HATs) and histone deacetylases (HDACs), determines whether the chromatin is open or closed, thereby controlling access of DNA-binding proteins for transcriptional activation. In this review, we summarize histone acetylation changes in plant response to drought stress, and review the functions of HATs and HDACs in drought response and resistance.

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“…The Arabidopsis class I (HDA19) and class II (HDA5/14/15/18) REDUCED POTASSIUM DEPENDENCY3 (RPD3)-like family histone deacetylases control positive and negative salinity stress responses, respectively, by acting conversely in the expressions of salt-responsive genes, such as ABI5 , NCED4 , GA20x7 , LEA4 _ 2 , P5CS1 , and NAC016 / 019 [ 69 , 70 ]. In addition, the enhanced gene expression of six histone deacetylases HcHDA2, HcHDA6, HcHDA8, HcHDA9, HcHDA19, and HcSRT2 in kenaf ( Hibiscus cannabinus ) during salinity stress is associated with the levels of H3K9ac, H3K27ac, and H4K5ac, but has not yet been linked to other stress-induced transcriptional responses [ 100 ]. The Arabidopsis floral initiator Shk1 kinase binding protein1 (SKB1), a type II arginine methyltransferase, regulates transcription and pre-mRNA splicing of FLOWERING LOCUS C ( FLC ) and the stress-responsive genes, such as HAB1 , MEK1 , MEKK1 , and MRK1 , during salinity stress by dissociating from chromatin and altering the methylation status of histone 4 arginine 3 (H4R3) symmetric demethylation (H4R3sme2) and small nuclear ribonucleoprotein Sm-like4 (LSM4) [ 71 ].…”

Section: Chromatin Dynamics Associated With Abiotic Stress-inducedmentioning

confidence: 99%

Multifaceted Chromatin Structure and Transcription Changes in Plant Stress Response

Kim

2021

IJMS

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Sessile plants are exposed throughout their existence to environmental abiotic and biotic stress factors, such as cold, heat, salinity, drought, dehydration, submergence, waterlogging, and pathogen infection. Chromatin organization affects genome stability, and its dynamics are crucial in plant stress responses. Chromatin dynamics are epigenetically regulated and are required for stress-induced transcriptional regulation or reprogramming. Epigenetic regulators facilitate the phenotypic plasticity of development and the survival and reproduction of plants in unfavorable environments, and they are highly diversified, including histone and DNA modifiers, histone variants, chromatin remodelers, and regulatory non-coding RNAs. They contribute to chromatin modifications, remodeling and dynamics, and constitute a multilayered and multifaceted circuitry for sophisticated and robust epigenetic regulation of plant stress responses. However, this complicated epigenetic regulatory circuitry creates challenges for elucidating the common or differential roles of chromatin modifications for transcriptional regulation or reprogramming in different plant stress responses. Particularly, interacting chromatin modifications and heritable stress memories are difficult to identify in the aspect of chromatin-based epigenetic regulation of transcriptional reprogramming and memory. Therefore, this review discusses the recent updates from the three perspectives—stress specificity or dependence of transcriptional reprogramming, the interplay of chromatin modifications, and transcriptional stress memory in plants. This helps solidify our knowledge on chromatin-based transcriptional reprogramming for plant stress response and memory.

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Molecular cloning and subcellular localization of six HDACs and their roles in response to salt and drought stress in kenaf (Hibiscus cannabinus L.)

Cited by 36 publications

References 52 publications

H3K14 hyperacetylation‑mediated c‑Myc binding to the miR‑30a‑5p gene promoter under hypoxia postconditioning protects senescent cardiomyocytes from hypoxia/reoxygenation injury

H3K14 hyperacetylation‑mediated c‑Myc binding to the miR‑30a‑5p gene promoter under hypoxia postconditioning protects senescent cardiomyocytes from hypoxia/reoxygenation injury

Histone Acetylation Changes in Plant Response to Drought Stress

Multifaceted Chromatin Structure and Transcription Changes in Plant Stress Response

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